Free flow electrophoresis apparatus

An electrophoretic device, a carrier-free technology, applied in the direction of measuring devices, solid separation, electrostatic effect separation, etc., can solve the problems of increasing the length of the electrode, manufacturing the separation chamber gap, increasing the external size of the separation chamber, etc.

Inactive Publication Date: 2005-12-21
BECTON DICKINSON & CO
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Problems solved by technology

But because the linear flow rate cannot be reduced arbitrarily, it is necessary to prolong the separation time and correspondingly increase the electrode length
This in turn means simultaneously increasing the outer dimensions of the separation chamber, which makes it difficult or even impossible to manufacture the separation chamber gap with the required precision

Method used

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  • Free flow electrophoresis apparatus
  • Free flow electrophoresis apparatus
  • Free flow electrophoresis apparatus

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Embodiment Construction

[0020] Three examples of separation chambers in different embodiments of an electrophoretic device according to the invention are schematically represented in FIGS. 1 , 2 and 3 . In order for the separation chamber to have external dimensions which allow machining of the separation chamber gap with the necessary precision, a plurality of separate separation spaces are provided in the separation chamber. According to FIG. 1 , there are four separate separation spaces with four separate medium inlets and four separate classification sections, each classification section having n classification positions, where n is less than 15. FIG. 2 shows a separation chamber with two separate separation spaces and two separate medium inlets, each with n classification positions, where n is greater than 50, on the two separate classification sections. Finally Fig. 3 shows a separation chamber with three separate separation spaces, three separate medium inlets, two classification sections with...

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Abstract

In a non-carrier electrophoresis process to separate a sample substance into its constituent parts, the sample is first subjected to coarse fractionation followed by a second-stage fine fractionation. In the first stage is conducted at high linear flow speed over a short migration distance. A smaller number of fractionating points is used in the first stage than in the second stage. The process stages may be conducted in parallel, in series, or in a combination of parallel and series. An independent claim is also included for an electrophoresis apparatus with a separation chamber, electrodes, sample and fraction sections. The separation chamber is sub-divided into smaller chambers with electrodes each serving two fraction chambers,.

Description

technical field [0001] The present invention relates to a carrier-free electrophoresis method for separating a sample substance from an analyte of the sample substance and an electrophoresis device for carrying out the method. Background technique [0002] The known carrier-free electrophoretic method FFE usually works with an electrophoretic device whose separation chamber has only two separating electrode spaces and a separating space between these electrode spaces. [0003] However, when the FFE is applied in the field of proteomics research, it must be able to separate many different sample substances with the largest possible separation capacity and the largest possible sample throughput in a short time. [0004] But in FFE, as in usual separation methods, the simultaneous optimization of the electrophoretic device in terms of separation capacity and sample throughput is only possible within very narrow limits, since an increase in the amount of sample substance will le...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447B01D57/02B03C5/00
CPCB01D57/02G01N27/44769G01N27/44773G01N27/447
Inventor 格哈德·韦伯
Owner BECTON DICKINSON & CO
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