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Self-balanced connected device for collecting separated liquid in use for electrophoresis of free stream

A collection device and separation liquid technology, which is applied in the field of free-flow electrophoresis separation liquid self-balanced communication collection device, can solve the problems of less use and enrichment of FFE, high cost of FFE, and single separation mode of FFE, so as to reduce production cost and reduce manufacturing cost. Costs, Effects of Removing Dependencies

Active Publication Date: 2005-11-16
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Expensive for high precision and high stability multichannel pumps, which largely contributes to the high cost of FFE
However, if a general constant flow pump is used, it is difficult to use a general constant flow pump for high-precision and high-stability liquid delivery in FFE due to the existence of unstable factors such as obvious pressure pulses.
[0004] Second, the FFE separation mode is single
[0005] Third, FFE is rarely used for sample enrichment
This new MCRB-mediated enrichment model is difficult to complete the relevant application research in the traditional FFE

Method used

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  • Self-balanced connected device for collecting separated liquid in use for electrophoresis of free stream
  • Self-balanced connected device for collecting separated liquid in use for electrophoresis of free stream
  • Self-balanced connected device for collecting separated liquid in use for electrophoresis of free stream

Examples

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Effect test

Embodiment 1

[0030] Embodiment 1: A small-volume self-balancing communicating collection device. The connecting bottle 1 and the collection bottle 14 of the device are designed between 100-1000mL. Correspondingly thereto are a small three-way pipe 2, an input pipe 3, an output pipe 4 and a rigid support 5. This device is mainly used for the separation and analysis of small-scale preparative proteins / cells / viruses, etc. in laboratories.

Embodiment 2

[0031] Example 2: Medium-volume self-balancing communicating collection device. The connecting bottle 1 and the collection bottle 14 of the device are designed between 1000-10000mL. Corresponding to it is the tee pipe 2, the input pipe 3, the output pipe 4 and the rigid support 5 of the medium model. This device is mainly used for the separation of medium-scale preparative proteins / cells / viruses in laboratories and enterprises.

Embodiment 3

[0032] Embodiment 3: Large-volume self-balancing communicating collection device. The connecting bottle 1 of this device and the design of collection bottle 14 are more than 10000mL. Correspondingly thereto are a large-scale tee pipe 2, an input pipe 3, an output pipe 4 and a rigid support 5. This device is mainly used for the separation of large-scale preparative proteins / cells / viruses in laboratories and enterprises.

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Abstract

A self-balanced separated liquid collector for free flow electrophoresis (FFE) has multiple specimen bottles which are installed on a rigid frame and communicated to the FFE separation chamber via respective inlet tube. The level meter and level regulating knob are used to regulate their level position. It can be used for conventional FFE separation of nucleic acid, protein, polypeptide, cell, virus, etc.

Description

technical field [0001] The invention relates to a self-balanced communication collection device for free-flow electrophoresis (Free-flow electrophoresis, FFE) separation liquid, which is used for automatic balance adjustment of the flow rate of the effluent in the FFE separation chamber. It belongs to the field of biochemical technology. Background technique [0002] Free-flow electrophoresis technology is a high-efficiency preparative separation technology for proteins. It uses a transverse electric field to separate and analyze proteins, viruses and cells in a continuously flowing electrolyte solution. Since the 1970s, FFE has been widely used in the isolation and analysis of genes, drugs, proteins, cells, organelles, and viruses. FFE has many advantages, such as the theoretical recovery rate is close to 100%, and it can be continuously injected for the separation of proteins / cells / viruses, etc. But there are still many problems. [0003] First, FFE is expensive. In FF...

Claims

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Application Information

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IPC IPC(8): C07K1/26
Inventor 曹成喜徐研杰张薇
Owner SHANGHAI JIAO TONG UNIV
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