Separation chamber device used for preparative free-flow electrophoresis

Abstract

A separation chamber device used for preparative free-flow electrophoresis (FFE) comprises a first synthetic glass flat plate, a second synthetic glass flat plate, an electrophoresis chamber and an electrode chamber; the electrophoresis chamber and the electrode chamber, which are formed by a separation hole and an electrode hole of an ion-exchange membrane, and a plastic film respectively, are clamped between the first and the second synthetic glass flat plates; a plastic screw passes through an upper screw hole on the first synthetic glass flat plate, a lower screw hole on the second synthetic glass flat plate and a film screw hole of the plastic film to be fixed; and an electrode buffer solution inlet and an electrode buffer solution outlet are formed on the first synthetic glass flat plate and at the positions corresponding to two ends of the electrode hole of the ion-exchange membrane and are used for circulating electrode solution. The invention can solve the problems of leak tightness and stability of the traditional FFE, provides a high-stability and high-uniformity hydrodynamics environment, is extensively applied to conventional FFE separation of various samples such as nucleic acid / protein / polypeptide / virus, and can be applied to various sample purifications carried out in FFE devices.

Description

technical field [0001] The present invention relates to the technical field of biochemical devices, in particular to a separation chamber device for preparative free-flow electrophoresis (hereinafter referred to as FFE). Background technique [0002] Because electrophoresis (Electrophoresis) technology can separate many biomolecules, such as proteins, peptides and cells, it has been widely used in the field of biochemistry. With the development of electrophoresis technology, the supporting medium in the electrophoresis process is gradually replaced by gel from the initial filter paper and cellulose acetate membrane. The introduction of gel as a supporting medium has greatly promoted the development of electrophoresis technology, making electrophoresis technology one of the important means for analyzing biological macromolecules such as proteins and nucleic acids. However, electrophoresis filled with solid media has many disadvantages. First of all, the content of the proce...

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Problems solved by technology

However, the preparation of this polyacrylamide membrane before electrophoresis operation is quite tedious, and the separation chamber is not well sealed, which reduces the operating efficiency of FFE

Moreover, the inherent properties of this membrane make the life of this electrophoretic separation chamber very short

In addition, the electrical conductivity of this film material is average, resulting in a relatively low effective voltage utilization.

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