Automatic heat dissipation type free-flow electrophoresis separation chamber device

Abstract

The invention discloses a free-flow electrophoresis separation chamber device used in the technical field of biochemical engineering. The device comprises an organic glass flat plate, a ceramic flat plate, an ionic membrane, an electrophoresis chamber, an electrode chamber, a fastening sealed structure and an automatic semiconductor refrigeration and heat dissipation device, wherein the ionic membrane is located between an upper organic glass flat plate and a lower ceramic flat plate, and the middle and two sides of the ionic membrane are hollowed, and the ionic membrane, the upper organic glass flat plate and the lower ceramic flat plate form the electrophoresis chamber and the electrode chamber; a plurality of liquid inlet holes are uniformly formed on one end of the upper organic glass flat plate, and double amount of liquid outlet holes are uniformly formed on the other end of the upper organic glass flat plate, thus forming a swimming lane. The device disclosed by the invention can effectively solve the problem that a separation chamber is easy to leak, can effectively eliminate joule heat in the separation chamber, can improve the stability of flowing shape of the fluid in the separation chamber, and is easy to assemble; the device can be widely used for routine free-flow electrophoresis separation of multiple types of samples such as small-molecule substances, protein, organelle and virus, and meanwhile, the device can be applied to purification of samples.

Description

technical field [0001] The invention relates to the technical field of biochemical devices, in particular to an automatic heat dissipation free-flow electrophoresis separation chamber device for preparative free-flow electrophoresis. Background technique [0002] The development of electrophoresis technology began in the early 20th century. This technology has been successfully used for the separation of various types of substances, such as organelles, proteins, polypeptides, amino acids, etc., and has been widely used in chemical biology and other fields. The support media often used in the early electrophoresis process include filter paper and cellulose acetate membrane, etc., with the development of technology, such support media are gradually replaced by gel. The advantages of easy forming of gel, easy adjustment of pore size, and the ability to recycle separated substances have greatly promoted the development of electrophoresis technology, making electrophoresis techno...

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Problems solved by technology

However, this series of devices has the following disadvantages: 1. The polyacrylamide gel membrane is very inconvenient in the electrophoresis operation; 2. The electrical conductivity of the polyacrylamide gel membrane generally leads to low voltage efficiency and short use time; 3. Fastening It is difficult for the device to ensure the sealing of the separation chamber; 4. The assembly of the separation chamber is inconvenient; 5. The upper and lower plates of this type of separation device are basically made of plexiglass plates, and at the same time a cooling water cooling system is added for heat dissipation, and due to the thermal conductivity of the plexiglass plate Very low, the temperature of the cooling water needs to be very low to dissipate the Joule heat

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