Off-Gel free flow electrophoresis coupling chip and making method thereof

Abstract

The invention relates to an Off-Adhesive free flow electrophoresis coupling chip and a making method thereof, belonging to the technical field of microfluidic chip manufacture. The chip comprises a chip substrate, an electrode and a separating chamber, wherein an immobilized pH gradient adhesive tape area and hard substrates for fixing and embedding of immobilized pH gradient adhesive are arranged at the tail end of the separating chamber. The method comprises the following steps: arranging a rectangular area for embedding of the immobilized pH gradient adhesive tape at the tail end of the separating chamber of the other substrate, then aligning the chip substrate with another substrate, carrying out sealing connection on the two substrates and the electrode according to a combined sealing method of the free flow electrophoresis chip to form a free flow electrophoresis chip, arranging the chosen immobilized pH gradient adhesive tape at the rectangular slotting area of the chip, and pressing and fixing the immobilized pH gradient adhesive tape by using the other hard substrate for using. The chip not only solves the defects of the free flow electrophoresis chip and Off-Adhesive electrophoresis technologies, but also inherits the advantages of the two technologies; and the chip has simple manufacturing method, and the immobilized pH gradient adhesive tape is replaceable.

Description

technical field [0001] The invention belongs to the technical field of processing and manufacturing microfluidic chips, and relates to an Off-Gel free flow electrophoretic coupling chip and a manufacturing method thereof. Background technique [0002] Free-flow electrophoresis (Free-Flow Electrophoresis, FFE) is a continuous separation preparation technology. Its basic principle is: a thin rectangular cavity is formed by two upper and lower parallel plates as a separation chamber, and the compound to be analyzed is separated from the cavity along with the carrier electrolyte. One end of the chamber flows in, and a separation voltage is applied in the vertical direction of the fluid. The sample is deflected in its flow direction according to its charged state, and the separated compound is collected at the outlet end. The technology has the following advantages: mild separation conditions, no solid support medium, continuous separation and preparation, etc. Because of these ...

AI Technical Summary

Problems solved by technology

Due to the characteristics of the technology itself, although the free-flow electrophoresis chip has mild conditions and can be prepared continuously, it is easily affected by the external environment and has poor reproducibility; while the Off-Gel electrophoresis technology has good reproducibility, it cannot be achieved like a free-flow electrophoresis chip. Continuous preparation of samples

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