Free flow electrophoresis chip of adhesive tape and processing method thereof

Abstract

The invention discloses a free flow electrophoresis chip of an adhesive tape and a processing method thereof, and relates to a simple micro-fluidic chip and a processing method thereof. The preparation method comprises the steps of: 1, cleaning and processing upper and lower substrates, pasting the designed chip structure patterns on the upper and lower substrates as alignment marks; 2, punching a liquid inlet hole on the upper substrate, and pasting a single sticky tape by aiming a separation chamber mark; 3, pasting a double sticky tape to the lower substrate, calibrating the pattern mark by a nicking tool under a microscope, removing excessive adhesive tape, and then pasting the single sticky tape and a conductive adhesive tape by aiming the mark; 4, removing a surface film of the double sticky tape on the lower substrate, and cementing the upper substrate and the lower substrate by alignment. The free flow electrophoresis chip of the adhesive tape does not need to use any expensive special device under a common process environment, and is simple in process, and low in construction cost, and can meet the accuracy of a certain structure.

Description

technical field [0001] The invention relates to a simple microfluidic chip and a processing method thereof, in particular to a tape free flow electrophoresis chip and a processing method thereof. Background technique [0002] Microfluidic chip system is a high-tech field developed in recent years. It integrates liquid storage and mixing pools in traditional biological and chemical analysis laboratories, channels for electrophoretic separation and analysis, and pumps and valve components for controlling liquids. , and sample detectors are integrated on a chip of several square centimeters to perform functional operations such as sample preparation, separation, detection, mixing, and reaction. The microfluidic chip system has the advantages of high efficiency, low power consumption, and miniaturization, so it has great application potential in the fields of biochemical analysis, food testing, and environmental monitoring. Microfluidic chips for various purposes and their proc...

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Problems solved by technology

However, as the height of the separation chamber of the free-flow electrophoresis chip decreases to tens or even tens of microns, the wall of the hydrophobic and highly elastic PDMS material is easily attached to the side of the glass wall, making it difficult to enter the liquid and increasing the difficulty of the separation operation.

Polymer chips are mainly prepared using PMMA (commonly known as plexiglass) plates. Usually, microstructures are made on PMMA by molding and hot pressing, and the sealing of the microstructure and the two PMMA sheets on the top cover is completed by thermocompression bonding. It will lead to deformation of the closed microstructure formed by sealing, especially the two-dimensional free-flow electrophoresis chip will have serious collapse in the separation area, and there is no report on the PMMA free-flow electrophoresis chip.

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