60 results about "Enterobacter cloacae subsp. dissolvens" patented technology

The invention discloses enterobacter cloacae and application of the enterobacter cloacae. The enterobacter cloacae SXH-1 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6296. The invention also protects the application of the enterobacter cloacae SXH-1 to the plant growth promotion. The plant is concretely chives. The enterobacter cloacae SXH-1 provided by the invention can use ACC (aminocyclopropane carboxylic acid) as the unique nitrogen source for growth, and meanwhile, the ACC is decomposed. The strain is hopeful to realize important effects in the chive growth promotion aspect.

The invention discloses enterobacter cloacae having a phosphorus solubilizing function and used for degrading imazethapyr and relates to enterobacter cloacae. The enterobacter cloacae have the functions of degrading imazethapyr and degrading indissolvable phosphorus into soluble phosphorus, thereby laying a foundation for solving the problem of soil pollution caused by residues of a herbicide imazethapyr by means of micro-biological degradation. The enterobacter cloacae T02 are preserved in CGMCC with the preserving address: #3, Yard 1, Beichen West Road, Chaoyang District, Beijing on Jule 9, 2014 and have a preservation number: CGMCC No. 9287. The enterobacter cloacae T02 not only have the function of degrading imazethapyr, but also have the function of degrading indissolvable phosphorus into soluble phosphorus. The enterobacter cloacae are used for degrading residual herbicide imazethapyr in soil.

The invention discloses a dominant consortium flora for degrading urban waste water and a preparation method thereof. The preparation method comprises the following steps of: inoculating and culturing enterobactercloacae, gordonia and pseudomonasputida respectively; mixing 20-24 percent by volume of enterobactercloacae, 54-58 percent by volume of gordonia and 26-18 percent by volume of pseudomonasputida respectively; and preparing calcium alginate immobilized particles, activating, and directly putting into urban waste water. By coordinating among strains, the TOC (Total Organic Carbon) degrading efficiency of urban waste water by microbial treatment can be increased effectively.

The invention relates to a primer for detection, specifically relates to a PCR (polymerase chain reaction) detection primer for detecting enterobacter cloacae and belongs to the technical field of biological detection. The enterobacter cloacae specific PCR detection primer comprises a forward primer and a reverse primer, wherein the forward primer F is 5'-CATGACACCGGTGTTTCCCCAGT-3'; and the reverse primer R is 5'-CGGTCGGTGAAGCCCAGAACCACTA-3'. A detection method for detecting the enterobacter cloacae, disclosed by the invention, has the advantages that the required detection time is short, the specificity is strong and the sensitivity is high. The PCR detection primer disclosed by the invention can avoid the shortcomings of trivial operation, long time, low accuracy, low detection rate and the like of a traditional identification method.

The invention discloses a bagasse immobilized bacterium and application thereof to sulfamethoxazole pollution remediation of farmland soil. According to the bagasse immobilized bacterium, Enterobacter cloacae is immobilized by adopting bagasse, and when the Enterobacter cloacae is inoculated to a bagasse vector, a bacterial suspension is prepared by culturing in advance, and then the Enterobacter cloacae is immobilized onto the bagasse vector treated by an inorganic salt culture solution to establish an efficient and stable agricultural waste immobilized bacterium system. The bagasse immobilized bacterium is low in cost, and when the soil is remediated, simplicity and environment-friendliness are achieved, and secondary pollution is avoided; in addition, the bioavailability of sulfonamides can be improved, and the microbiological degradation efficiency of the sulfonamides is improved.

The invention discloses saline-alkaline tolerant enterobacter cloacae and a production method and application of a viable bacterium preparation of the saline-alkaline tolerant enterobacter cloacae. The saline-alkaline tolerant enterobacter cloacae ZTS-10 is separated from rhizosphere soil of chili peppers in saline-alkaline land, the preservation number of the bacterial strain in the China GeneralMicrobiological Culture Collection Center (CGMCC) is CGMCC No.20181, and the preservation date is 3 July, 2020. The bacterial strain is high in saline-alkaline tolerance and high in field planting rate in the saline-alkaline soil, has the capacity of producing IAA, degrading phosphorus and degrading potassium, can promote growth of the chili peppers, has good antagonism effects on pathogenic bacteria of chili pepper epidemic diseases, can be used for producing microorganism fertilizers especially microorganism fertilizers special for the chili peppers in the saline-alkaline land, can reduce the generation of chili pepper epidemic diseases, and can increase yield and quality.

The invention discloses enterobacter cloacae with the aerobic denitrification function and an application of the enterobacter cloacae in wastewater treatment. The enterobacter cloacae are enterobacter cloacae HNR and are collected to the China General Microbiological Culture Collection Center (CMGCC) on January 13th, 2015, the collecting number is CGMCC No.10348. According to the enterobacter cloacae, under the aerobic condition, NO3<->-N can be converted into a gaseous product through the aerobic denitrification effect with organic carbon as the unique carbon source and the NO3<->-N (nitrate nitrogen) as the unique nitrogen source, and synchronous removing of nitrogen elements and the organic carbon is achieved. According to the enterobacter cloacae, the removing rate for the NO3<->-N is as high as 90% to 98 %, the removing rate for the total nitrogen can be 85% to 95 %, and the removing rate for the total organic carbon can be higher than 85%. The enterobacter cloacae can be used for effectively removing the NO3<->-N in the organic wastewater, and have the effects that the whole process is aerobic, and the nitrogen removal efficiency is high.

The invention relates to Enterobacter cloacae with the preservation number of CGMCC No.16235 and application thereof in degradation of thiamethoxam. The Enterobacter cloacae can be prepared into a microbial agent, is applied to remediation of soil-derived thiamethoxam residue pollution in a leaf vegetable planting system, decreasing of thiamethoxam residues of leaf vegetables, and promotion of plant growth; the Enterobacter cloacae is common probiotic in soil, can be colonized in vegetable plants for a long time, and effectively decreases thiamethoxam residues on the premise that vegetable quality is not influenced, and green and safe production is achieved.

The invention relates to enterobacter cloacae which are found in soil and have the collection number of CGMCC No.2487 or resting cell with equivalent function, variant and mutant as well as enzyme produced thereby. The invention also provides a preparation method of taxane, namely comprising the following steps: the enterobacter cloacae are used for hydrolyzing 7-xylose group in 7-xylose taxane molecule in wheat bran culture medium, and 13-side chain in taxane molecule with 13-side chain can be hydrolyzed in peptone beef extract culture medium; and the produced taxane can be used for preparing antitumor drugs, such as intermediates of taxane drugs, docetaxel drugs or taxane drugs.

The invention discloses Enterobacter cloacae ZJPH1903 and application thereof in preparation of (R)-2-chloro-1-(2, 4-dichlorophenyl)ethanol. A method of catalytic preparation of optically pure (R)-2-chloro-1-(2, 4-dichlorophenyl)ethanol with the new strain has the advantages of good stereoselectivity, high product optical purity and the like, and when the substrate concentration is 4g / L, the e.e.Value of the target product (R)-2-chloro-1-(2, 4-dichlorophenyl)ethanol is greater than 99.9%, and the yield is 92.9%. When a surfactant is added into the reaction system, the catalytic efficiency canbe effectively improved, the concentration of the catalytic substrate can be increased to 10g / L, the yield of the target product (R)-2-chloro-1-(2, 4-dichlorophenyl)ethanol reaches 94.3%, and the e.e. Value is greater than 99.9%.

The invention discloses a LAMP primer group for detecting enterobacter cloacae, a kit and a rapid detection method. The LAMP primers are six specific primers designed according to a dnaJ gene of enterobacter cloacae. By applying the six primers, specific amplification can be realized, the enterobacter cloacae can be effectively identified, and the lowest detection limit can reach 1 DNA copy; a DNA sample can be detected within 60min; and the detection method can be flexibly selected according to actual conditions, and the primer group is convenient and rapid and has a very wide application prospect.

The invention discloses a method capable of reducing biogenic amine in a fermented sausage and application thereof. The method comprises the following steps that 1, according to sequences of amine production-correlated genes luxR of main amine production bacteria enterobacter aerogenes and enterobacter cloacae in the fermented sausage, small-interfering ribonucleic acid (siRNA) is designed and prepared; 2, the siRNA is used as an encapsulated matter for preparing lipidosome, so that the stable siRNA cationic liposome is obtained; 3, the siRNA cationic liposome is added into the fermented sausage according to a certain ratio in a fermentation early stage. According to the method, the flavor, color and lustre of the fermented sausage are not affected, and the effect of inhibiting the biogenic amine is significant, so that the method has important significance in controlling the biogenic amine in the fermented sausage.

The invention discloses an alga-lysing microbial agent and application thereof. The microbial agent contains enterobacter and / or an enterobacter extract, and the enterobacter is selected from enterobacter hormaechei F2 or enterobacter cloacae. The microbial algicidal inoculant contains the enterobacter hormaechei F2 and / or the enterobacter hormaechei F2 extract, and has an obvious dissolution effect on microcystis aeruginosa in a water body, the algicidal rate reaches 95%, the algicidal rate on filamentous algae reaches 97%, the removal rate of algal cells in an actual eutrophic water body can reach 87.32%, and the removal rate of the microcystis aeruginosa in the actual eutrophic water body can reach 87.32%. The alga-lysing microbial agent has great application in water eutrophication treatment.

The invention discloses bacterium with an induced resistance activity and a cultural method and application thereof, wherein the bacterium is enterobacter cloacae and the preservation number of the enterobacter cloacae is CGMCC No. 10801. The bacterium with an induced resistance activity or the metabolite thereof can be used for preparation of a soybean seed treating agent and biological prevention and control of plant parasitic nematodes. The enterobacter cloacae (E.cloacae) Sneb572 or the metabolite thereof has an activity of inducing soybeans, can be used for soybean seed treatment, therefore not only is the growth of soybean plants and roots promoted, but also a great inhibiting effect on formation of heterodera glycines cysts is achieved, and the problem of prevention and control of heterodera glycines is solved.

The invention provides lactobacillus plantarum SLB01 with an inhibiting effect on enterobacter cloacae as well as a derivative product and application of the lactobacillus plantarum SLB01, and belongs to the technical field of microorganisms. The invention provides Lactobacillus plantarum SLB01 with an inhibition effect on enterobacter cloacae, and the preservation number of the Lactobacillus plantarum SLB01 is GDMCCNo: 61178. In-vitro bacteriostasis experiments and in-vivo bacteriostasis experiments show that compared with conventional lactobacillus, the Lactobacillus plantarum SLB01 has a relatively strong inhibiting effect on enterobacter cloacae and has the characteristics of acid resistance and cholate resistance. According to the present invention, by continuously eating the food or the bacterial agent containing the Lactobacillus plantarum SLB01, the results show that the abundance of the enterobacter cloacae in the intestinal tract can be significantly reduced so as to achieve the purposes of regulating the intestinal flora balance and assisting the prevention and treatment of obesity and other diseases caused by the enterobacter cloacae.

The invention discloses enterobacter cloacae with capability of degrading a benzene series and application of the enterobacter cloacae in environmental restoration. According to the enterobacter cloacae and the application thereof, the enterobacter cloacae with good capability of degrading the benzene series is screened and separated from landfill leachate of a certain refuse landfill in Guangzhou, Guangdong; and the enterobacter cloacae GDUTAN7 is preserved in the China Center for Type Culture Collection (CCTCC) on January 18, 2021, the address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2021091. The enterobacter cloacae GDUTAN7 disclosed by the invention has a relatively good degradation effect on the benzene series in environments and can be applied to the degradation of the benzene series in the environments, and thus, the aim of restoring the environments is achieved.

The invention belongs to the technical field of microorganisms, and particularly relates to an enterobacter cloacae strain and application thereof. The enterobacter cloacae strain is preserved in the China General Microbiological Culture Collection Center on December 23 <rd>, 2020, and the preservation number is CGMCC NO. 21529. The enterobacter cloacae strain can promote plant root systems to synthesize auxin and promote plant lateral root development. Plant root system development promotion is specifically reflected in promoting generation and extension of plant lateral roots; plant growth and development promotion is specifically reflected in promoting increase of the fresh weight and yield of plants. The enterobacter cloacae strain can also be used for preparing auxin in a fermentation mode.

The invention relates to enterobacter cloacae for degrading petroleum pollution and application thereof. The enterobacter cloacae 2# provided by the invention has a preservation number of CGMCC No. 18937, and the strain (enterobacter cloacae 2#) has an effect of degrading petroleum. The strain can be applied to the treatment of petroleum pollution, and the strain can be removed through natural degradation after the petroleum is degraded by the strain, so that the strain can be used for the treatment of the petroleum-polluted environment without causing secondary pollution to the environment.

The invention discloses a preparation method and application of Leersia hexandra swartz endophytic bacteria with a hexavalent chromium reducing function. The strain has the classification naming of Enterobacter cloacae, and is collected in CGMCC (China General Microbiological Culture Collection Center) with the address of NO.3, NO.1 Yard, Beichen West Road, Chaoyang District, Beijing, 100101. Thecollection number is CGMCC NO.14265. The Leersia hexandra swartz endophytic bacteria are separated and screened from the root tissues of the chromium super accumulation plants of Leersia hexandra swartz, are identified as Enterobacter cloacae, and are named as G04. The strain can reduce hexavalent chromium into trivalent chromium; the chromium reducing conditions are cleared; the strain resource and the theoretical basis are provided for hexavalent chromium pollution microbial remediation.

The invention discloses an escherichia coli engineering bacterium for efficiently producing 2,3,5,6-tetramethylpyrazine. The engineering bacterium is an escherichia coli carrying an acetoin syntheticoperator alsRSD from Enterobacter cloacae and an NADH oxidase gene nox from Lactobacillus brevis. Using the genetic engineering bacterium provided by the invention to transform glucose to produce 2,3,5,6-tetramethylpyrazine, the highest yield is up to 16.40g / L, the purity of a product is more than or equal to 98%, and the engineering bacterium has the advantages of mild reaction conditions, few by-products, and easy separation and purification of the product, and has a very good application value.

The invention discloses a method for enhancing colonization of enterobacter cloacae Y16 in a plant root system. Research finds that when bacterial liquid of enterobacter cloacae Y16 and low-molecular organic acid are jointly applied to the rhizosphere soil of a heavy metal hyperaccumulation plant, the formation of a biological membrane of the enterobacter cloacae in the plant root system can be remarkably promoted, the colonization ability of the enterobacter cloacae is enhanced, the growth of the plant is promoted, and the efficiency of microorganism-plant combined remediation of the farmland soil polluted by medium-low-concentration cadmium is improved. The low-molecular organic acid is at least one of malic acid, citric acid or succinic acid which has a directional recruitment effect on the enterobacter cloacae Y16. According to the method, the remediation efficiency of the heavy metal hyperaccumulation plant on the medium-low heavy metal polluted farmland soil can be remarkably improved.

The invention relates to the field of uranium mine radioactive pollution treatment, in particular to enterobacter cloacae and application thereof. The collection number of the enterobacter cloacae is CGMCC (China General Microbiological Culture Collection Center) NO: The enterobacter cloacae is applied to uranium element reduction and uranium ore slag pollution treatment. The invention provides a preparation method of active bacterial liquid, which comprises the following steps: inoculating enterobacter cloacae into a liquid culture medium, standing and culturing to obtain a basic active bacterial agent; and inoculating the basic active bacterial agent into a fermentation medium according to an inoculation amount of 2-5%, and carrying out shake culture, so that the number of cells in each milliliter of the fermentation medium is not less than 3 * 10 < 9 >-5 * 10 < 9 >, thereby obtaining the active bacterial liquid. The strain has a reduction effect on uranium in tailings, uranium is converted into a reduction state which is not easy to dissolve from an oxidation state which is easy to dissolve, and the uranium is fixed in situ, so that the risk of migration to the external environment is reduced, and uranium pollution is inhibited from the source.

The invention discloses application of luteolin in inhibiting the growth of multi-drug resistant enterobacter cloacae. Luteolin has a good in-vitro killing effect on multi-drug resistant enterobactercloacae with resistance to cefazolin, cefotaxime, augmentin, meropenem, ofloxacin, levofloxacin, ciprofloxacin, cefoxitin, minocycline, imipenem, piperacillin, azithromycin, furantoin, sulfamethoxazole and nalidixic acid, can inhibit the growth of the multi-drug resistant enterobacter cloacae, and has a minimum bactericidal concentration of 0.5mg / mL and a minimum inhibitory concentration of 0.3mg / mL. The invention provides the inhibition effect of luteolin on the multi-drug resistant enterobacter cloacae, and has wide application value in the fields of medicine and the like.

The invention discloses a strain of salt-tolerant rhizosphere growth-promoting Enterobacter cloacae and its application. The strain is named Enterobacter cloacae (Enterobacter cloacae) TIA062, and has been preserved in China's Microbiological Culture Collection Management on January 7, 2016. Committee General Microbiology Center, deposit number is CGMCC No.11974. The salt-tolerant rhizosphere growth-promoting enterobacter cloacae of the invention can improve the salt-alkali tolerance of white wax. The salt-tolerant rhizosphere growth-promoting Enterobacter cloacae of the present invention is inoculated around the root system of white wax, and after a certain period of salt stress, it can increase the water content of the root system of white wax and promote the growth of white wax compared with no inoculation agent. Therefore, the present invention The invention can be used as an inoculant in a saline environment, and provides excellent strain resources for improving the saline-alkali resistance of white wax.

The invention discloses an E. coli engineering strain that can efficiently produce 2,3,5,6-tetramethylpyrazine. The engineered bacterium is Escherichia coli carrying the acetoin synthesis operon alsRSD derived from Enterobacter cloacae and the NADH oxidase gene nox derived from Lactobacillus brevis. The genetically engineered bacterium of the present invention is used to transform glucose to produce 2,3,5,6-tetramethylpyrazine, with a maximum yield of 16.40g / L, a product purity of ≥98%, mild reaction conditions, few by-products, and an easy-to-use product. The advantages of separation and purification have great application value.

The invention discloses an Enterobacter cloacae. Enterobacter cloacae (Enterobacter cloacae) DHT001 has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on May 28, 2018, and its preservation number is: CGMCC No.15820. Correspondingly, the invention also discloses the application of the Enterobacter cloacae in promoting the growth of ruminants, the feed containing the Enterobacter cloacae and the isolation method of the Enterobacter cloacae. The Enterobacter cloacae of the present invention can self-synthesize cysteine ​​in the rumen of ruminants, and the Enterobacter cloacae is mixed into feed to promote the growth of ruminants. The Enterobacter cloacae strain is an inherent bacterial species in the rumen of ruminants, and is a safe, efficient and environmentally friendly microbial additive.

The invention relates to a functional strain, and relates to a high-efficiency degrading phorbol ester strain and its application in detoxification of Jatropha curcas cake fermentation. A highly efficient phorbol ester degrading strain, the bacteria is Enterobacter cloacae ( Enterobacter cloacae ) strain Z11, which was collected on September 21, 2017 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures (Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip Code 100101) Deposited, the number is CGMCC No.14655. The invention can effectively reduce the content of toxic components and anti-nutritional factors in the Jatropha curcas cake, and can improve the feeding value of the Jatropha curcas cake.

The invention relates to a primer composition for detecting carbapenemase-resistant enterobacter cloacae OXA48 family genes through LAMP, a corresponding kit and a detection method, common gene segments of the OXA48 family are selected, the common gene segments comprise blaOXA-48, blaOXA-181, blaOXA-232, blaOXA-204, blaOXA-162 and blaOXA-244, primers needed in a loop-mediated isothermal amplification system are designed, and the primers are used for detecting carbapenemase-resistant enterobacter cloacae OXA48 family genes through LAMP. A reaction system, reaction conditions, coloring agent concentration and the like are repeatedly optimized, and the improved OXA48 family LAMP detection kit is established. The LAMP detection kit not only can be used for detecting OXA48 family genes of enterobacter cloacae, but also can be used for detecting other bacteria, and the bacteria containing the drug-resistant genes can be detected. The LAMP detection kit is simple and convenient to operate and low in operation risk.