34 results about "ENTEROBACTER HORMAECHEI" patented technology

The invention relates to a lignocellulose substance efficient degradation bacterium K24 and an application thereof. The lignocellulose substance efficient degradation bacterium K24 is enterobacter hormaechei K24 and is collected in the China General Microbiological Culture Collection Center (CGMCC) on December 12, 2014, with collection number of CGMCC No.10169. The lignocellulose substance efficient degradation bacterium K24 is used for degrading lignocellulose substances. The lignocellulose substance efficient degradation bacterium K24 has the beneficial effects that the strain is rapid in proliferation, has strong adaptability and is widely applied; the weight loss ratios of waste agaric material substitutes subjected to fermentation treatment with enterobacter hormaechei K24 are more than 39%; and the weight loss ratios of corn straws subjected to fermentation treatment with enterobacter hormaechei K24 are more than 29%.

The invention discloses a microbial flora for treating high-salt industrial wastewater and aims to screen and compound functional florae and verify the application effects on the basis of the characteristics that high-salt wastewater is high in salt content, high in chromaticity, high in COD, low in BOD and poor in biochemical property and contains complicated contaminants. Through isolation, screening and acclimatization, an active flora is obtained, the active flora has the capability of tolerating 18% high-salt wastewater and can be applied to biochemical treatment on high-salt pickling wastewater; according to the components and the proportion of the flora, relevant strains are purchased from the China General Microbiological Culture Collection Center and used for simulating the flora components of the acclimatized microbial flora, that is, enterobacter hormaechei, bacillusmegatherium, chromohalobacterjaponicas, halomonasxianhensis, chryseobacteriumindoltheticum and clostridiumsporogenes are compounded in a quantity ratio of 2:2:1:1:1:1, a relatively remarkable degradation effect is achieved when the compounded microbial flora is used for treating wastewater, COD in high-salt wastewater can be degraded to 1935.62mg/L from 29465mg/L, the COD degradation rate is 93.43%, and an effective technical support is provided for biological treatment on high-salt industrial wastewater which is hard to treat.

The invention belongs to the field of bioengineering and relates to a preparation method and application of a carotenoid 9,10' dioxygenase. The preparation method comprises the following steps: cultivating enterobacter hormaechei, and extracting a whole genome sequence; constructing a recombinant plasmid of a carotenoid 9,10' dioxygenase gene; inducing expression of the carotenoid 9,10'dioxygenase gene, and purifying zymoprotein; and analyzing and identifying enzymatic properties of the carotenoid 9,10' dioxygenase. The prepared carotenoid 9,10' dioxygenase gene can efficiently catalyze and synthesize beta-ionone, catalysis specificity is high, reaction products are few, and reaction time is short; a novel enzyme resource different from discovered carotenoid 9,10' dioxygenase is obtained; the carotenoid 9,10' dioxygenase has different amino acid sequences and enzymatic characteristics; and reaction conditions are optimized, and yield of the beta-ionone reaches 79.4%.

The invention relates to a lignocellulos substance high-efficiency degrading bacterium S1 and application thereof. The lignocellulos substance high-efficiency degrading bacterium S1 is Enterobacter hormaechei S1 and was preserved with preservation number CGMCC No.10165 on December 12, 2014 by China General Microbiological Culture Collection Center. The bacterium is used for degrading lignocellulos substances. The strain provided by the invention has the advantages of quick proliferation, strong applicability and wide application; a weight-loss ratio of bagged agaric wastes after being fermented by Enterobacter hormaechei S1 is above 31 percent and a weight-loss ratio of corn stalks after being fermented by Enterobacter hormaechei S1 is above 33 percent.

The invention discloses enterobacter hormaechei bacteriophage YZU.P.A-5 and application thereof. The bacteriophage is identified to have unique genome structure characteristics, and belongs to a novelkind of bacteriophage; and the bacteriophage has been preserved in China Center for Type Culture Collection since June 15, 2020 under the preservation number of CCTCC NO: M 2020206. The bacteriophagedisclosed by the invention can efficiently crack enterobacter hormaechei so as to effectively inhibit enterobacter hormaechei in various matrices; and thus, the bacteriophage can be applied to preparation of a bacterial depressant for controlling enterobacter hormaechei pollution. The bacterial depressant prepared by the bacteriophage can effectively control growth of enterobacter hormaechei in culture media and food matrices, as well as effectively inhibit formation of enterobacter hormaechei mycoderm on surfaces of equipment, utensils and raw material solids; and moreover, the bacterial depressant is simple to prepare and convenient to use, as well as capable of reducing risks of enterobacter hormaechei propagation and food-borne diseases.

The invention belongs to the technical field of biological hydrogen production, and particularly relates to a preparation method of a biomass photosynthetic hydrogen production mixed flora, and the method comprises the following preparation steps of: S1, preparing a bacterial growth culture medium; S2, amplifying a bacterial liquid; according to the novel photosynthetic bacteria, HAU-M1 photosynthetic bacteria and enterobacter hormaechei are used as co-culture hydrogen-producing bacteria, a growth medium and the co-culture hydrogen-producing bacteria are added into an anaerobic sealed culture container, and the mixture is placed under proper conditions for culture and amplification; and S3, conducting hydrogen production experiment. Enterobacter hormaechei is introduced into the HAU-M1 photosynthetic bacterium flora, the prepared photosynthetic bacterium mixed flora can secrete a rhamnolipid surfactant in the culture process, the hydrogen production amount of a light fermentation biological hydrogen production system is effectively increased, and the effect is obvious; when 6% of enterobacter hormaechei is added, the accumulated hydrogen production of light fermentation reaches a peak value and is far greater than that of a group without the enterobacter hormaechei; the method has the advantages of low cost, high efficiency, environmental friendliness, strong adaptability and the like, and has wide application prospects in the field of light fermentation biological hydrogen production.

The invention discloses a method for producing pimelic acid through fermentation of enterobacter hormaechei, and belongs to the technical field of microbial fermentation. The enterobacter hormaechei capable of producing pimelic acid is screened from preserved strains, the strain is a natural strain, antibiotics and inducers do not need to be added in the fermentation process, the industrial production cost is reduced, and meanwhile environment protection is facilitated. When the enterobacter hormaechei is fermented for 72 hours under a shake flask condition, the yield of pimelic acid is 115.4 mg/L; by optimizing the fermentation processes such as the carbon source type, the nitrogen source type, the carbon and nitrogen source combination, the inoculum size, the type and concentration of additional inorganic salt and the like, the yield of pimelic acid is further increased and reaches 217.3 mg/L. Meanwhile, a new host is provided for biological synthesis of pimelic acid, and a biological foundation is also laid for subsequent research.