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Application of combination of PQS and Enterobacter hormaechei in inhibition of growth and reproduction of algae

A technology of Enterobacter hallii and algae, applied in the field of microorganisms, can solve the problems of limiting the algae-dissolving effect of algae-lytic bacteria, single species, and limiting the application of biological algae removal, etc., so as to improve the algae inhibition effect, control flooding, and improve ROS activity. Effect

Active Publication Date: 2022-07-22
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the species of algicidal bacteria currently available in biological algae removal is single, which limits the algicidal effect of algicidal bacteria, thereby limiting the application of biological algae removal

Method used

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  • Application of combination of PQS and Enterobacter hormaechei in inhibition of growth and reproduction of algae
  • Application of combination of PQS and Enterobacter hormaechei in inhibition of growth and reproduction of algae
  • Application of combination of PQS and Enterobacter hormaechei in inhibition of growth and reproduction of algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The algae-lytic bacteriolytic agent in Example 1 is formed by mixing 2 mL of Enterobacter hallii F2 bacterial solution and PQS, wherein the final concentration of PQS is 2 μmol / L, and the effective viable count of Enterobacter hallii F2 is 1× 10 9 CFU / g.

Embodiment 2

[0057] The algae-lytic bacteriolytic agent in Example 2 is formed by mixing 2 mL of Enterobacter holleri F2 bacterial solution and PQS, wherein the final concentration of PQS is 10 μmol / L, and the effective viable bacterial count of Enterobacter hoelii F2 is 1× 10 9 CFU / g.

Embodiment 3

[0059] The algae-lytic bacteriolytic agent in Example 3 is formed by mixing 2 mL of Enterobacter hallii F2 bacterial solution and PQS, wherein the final concentration of PQS is 20 μmol / L, and the effective viable count of Enterobacter hallii F2 is 1× 10 9 CFU / g.

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Abstract

The invention discloses application of a combination of PQS and enterobacter hormaechei in inhibition of growth and reproduction of algae. The algicidal microbial inoculum comprises enterobacter hormaechei and PQS, and the enterobacter hormaechei is enterobacter hormaechei F2. The PQS in the algicidal bacterial agent does not have the functions of algicidal and algal inhibition, the algicidal effect of the enterobacter hormaechei F2 can be improved through the synergistic effect of the PQS and the enterobacter hormaechei F2, the algicidal rate reaches up to 83.81%, in addition, the content of chlorophyll a in an algicidal solution can be remarkably reduced while the biomass of the enterobacter hormaechei F2 is not affected through the addition of the PQS, and the content of chlorophyll a in the algicidal solution can be remarkably reduced. The activity of ROS (reactive oxygen species) in algae liquid can be remarkably improved, so that the flooding of algal bloom can be effectively controlled, and the method has a relatively great application prospect in the treatment of eutrophication of a water body.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the application of the combination of PQS and Enterobacter hallii in inhibiting the growth and reproduction of algae. Background technique [0002] In recent years, due to the large amount of nitrogen, phosphorus and other elements contained in wastewater and sewage, it has led to the growth of harmful algae such as microalgae, cyanobacteria, green algae, and diatoms in the water body. These harmful algae can gradually lose the self-purification ability of the water body and destroy natural landscape, causing the death of aquatic animals and even endangering human health. Therefore, the control of algae has become the main problem faced in water pollution control. [0003] Common methods for controlling algae include physical, chemical and biological methods. The physical method is mainly to remove algae by mechanical sieving, forced interception and filtrati...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C02F3/34C12R1/01
CPCC12N1/20C12N1/38C02F3/34
Inventor 杨美琦张斌吕烈阳何维谢文嘉刘伟
Owner SUN YAT SEN UNIV
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