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Microbial flora for treating high-salt industrial wastewater

A technology of microbial flora and flora, applied in the food industry wastewater treatment, microorganism-based methods, microorganisms, etc., can solve the problems of no industrialized scale, unstable activity, high fermentation cost, and reduce environmental toxicity and production. Low cost and adaptable effect

Active Publication Date: 2015-03-04
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Screening and separating it by modern biological means, and making it into a biological agent for the treatment of saline chemical wastewater has become a research hotspot, but the biochemical treatment of bacteria for high-salt industrial wastewater is still limited to laboratory research in China, and fermentation High cost, unstable activity, low efficiency, no industrialization scale, and a blank market

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  • Microbial flora for treating high-salt industrial wastewater
  • Microbial flora for treating high-salt industrial wastewater
  • Microbial flora for treating high-salt industrial wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Identification of community structure for treating high-salt sewage

[0020] The mud and water samples extracted from the wastewater pool of the pickle factory were packed into sterile sampling bags and brought back to the laboratory. Microscopic observation found that the samples had many microbial cells, which were suitable for the isolation and screening of salt-tolerant bacteria. Put the soil sample or water sample into the aerobic aeration reaction tank for continuous cultivation, mix with the pre-prepared inorganic salt medium, aerate for a long time, keep the oxygen content at 8-12mg / L, and cultivate at 30°C , Salt concentration 15% (as NaCl). The composition of the inorganic salt medium is as follows (w / v): potassium nitrate 0.3%, potassium dihydrogen phosphate 0.1%, ammonium dihydrogen phosphate 0.1%, ammonium sulfate 0.1%, pH 7.5, the carbon source in the bacterial flora screening system comes from sewage According to the test results, when the COD...

Embodiment 2

[0022] Example 2: Single bacterium compound flora and activity verification

[0023] According to the active functional flora screened in Example 1, related strains were purchased from China General Microorganism Culture Collection Center (CGMCC). The single bacteria were activated with LB medium, and the LB solid medium was also used for strain antagonism experiments to exclude the antagonized strains and ensure the normal growth of the compound flora. Enterobacter hormaechei (CGMCC 1.10608) isolated from fermented mattress materials, Bacillus megaterium (CGMCC 1.2764) used to treat organic waste, and Chromohalobacter japonicas isolated from pond water were selected respectively. CGMCC 1.9037), Halomonas xianhensis (CGMCC 1.6848) isolated from oil-contaminated soil, Chryseobacterium indoltheticum (CGMCC 1.10984) isolated from sea mud, and Clostridium sporogenes (CGMCC) 1.2157), adjust the cell concentration after activation, simulate the composition ratio of the microbial co...

Embodiment 3

[0024] Example 3 Application of compound active flora in pickling wastewater

[0025]A large amount of salt is added to the pickles during the pickling process, resulting in high salinity in the wastewater generated during the pickling process, generally between 15-20%, and high nitrite, ammonia, nitrogen, and phosphorus substances. If this kind of high-salt, high-nitrogen and phosphorus wastewater is mixed with domestic sewage and discharged into the sewage pipe network, it will seriously affect the stable operation of the sewage treatment system. The water quality of the selected pickling wastewater is turbid, dark brown, with high chroma, COD as high as 29465mg / L, and salinity 18%. The water quality parameters are shown in Table 1.

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Abstract

The invention discloses a microbial flora for treating high-salt industrial wastewater and aims to screen and compound functional florae and verify the application effects on the basis of the characteristics that high-salt wastewater is high in salt content, high in chromaticity, high in COD, low in BOD and poor in biochemical property and contains complicated contaminants. Through isolation, screening and acclimatization, an active flora is obtained, the active flora has the capability of tolerating 18% high-salt wastewater and can be applied to biochemical treatment on high-salt pickling wastewater; according to the components and the proportion of the flora, relevant strains are purchased from the China General Microbiological Culture Collection Center and used for simulating the flora components of the acclimatized microbial flora, that is, enterobacter hormaechei, bacillusmegatherium, chromohalobacterjaponicas, halomonasxianhensis, chryseobacteriumindoltheticum and clostridiumsporogenes are compounded in a quantity ratio of 2:2:1:1:1:1, a relatively remarkable degradation effect is achieved when the compounded microbial flora is used for treating wastewater, COD in high-salt wastewater can be degraded to 1935.62mg / L from 29465mg / L, the COD degradation rate is 93.43%, and an effective technical support is provided for biological treatment on high-salt industrial wastewater which is hard to treat.

Description

technical field [0001] The invention belongs to the technical field of screening and compounding of functional microbial flora, and in particular relates to a microbial flora for treating high-salt industrial wastewater and its application. Background technique [0002] The salinity in industrial wastewater is high, some of which exceed 20% (w / v), and the composition is complex, mostly containing alkanes, aromatics, carbohydrates, lipid compounds and nitrogen-containing compounds, as well as a large number of soluble inorganic salts, such as Cl - , SO 4 2- 、Na + and Ca 2+ Wait. High concentrations of inorganic salts will inhibit and poison the microorganisms in the sludge, cause the microorganisms to lose their degradation ability, and destroy the bacterial structure of the activated sludge, and even cause the collapse of the biochemical treatment process. There are always problems such as high cost, low efficiency, long cycle, poor stability, and secondary pollution, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/11C12R1/01C12R1/145
CPCC02F3/34C02F2103/32C12N1/20
Inventor 马皛梅黄志勇蔡明昱侍浏阳万桂龙王兴彪
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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