Preparation method of biomass photosynthetic hydrogen production mixed flora

Abstract

The invention belongs to the technical field of biological hydrogen production, and particularly relates to a preparation method of a biomass photosynthetic hydrogen production mixed flora, and the method comprises the following preparation steps of: S1, preparing a bacterial growth culture medium; S2, amplifying a bacterial liquid; according to the novel photosynthetic bacteria, HAU-M1 photosynthetic bacteria and enterobacter hormaechei are used as co-culture hydrogen-producing bacteria, a growth medium and the co-culture hydrogen-producing bacteria are added into an anaerobic sealed culture container, and the mixture is placed under proper conditions for culture and amplification; and S3, conducting hydrogen production experiment. Enterobacter hormaechei is introduced into the HAU-M1 photosynthetic bacterium flora, the prepared photosynthetic bacterium mixed flora can secrete a rhamnolipid surfactant in the culture process, the hydrogen production amount of a light fermentation biological hydrogen production system is effectively increased, and the effect is obvious; when 6% of enterobacter hormaechei is added, the accumulated hydrogen production of light fermentation reaches a peak value and is far greater than that of a group without the enterobacter hormaechei; the method has the advantages of low cost, high efficiency, environmental friendliness, strong adaptability and the like, and has wide application prospects in the field of light fermentation biological hydrogen production.

Description

Technical field [0001] The present invention relates to the field of biomarbon hydrogen, and is specifically a method for producing a biomass photosynthetic hydrogen production mixed gel. Background technique [0002] The increasing depletion of fossil energy and its use of environmental pollution forces people to develop new clean renewable energy sources to meet future economic and social development needs. Hydrogen can be recognized in the form of a major energy carrier in the future due to high energy density and non-pollution and use form. The development model of "hydrogen energy economy" and "hydrogen energy society" with hydrogen energy can be used in the future of future energy use technology. [0003] Bihydrogenation is the process of releasing hydrogen using microbial self-metabolism, which is mild, environmentally friendly, and is considered to be the main alternative to future hydrogen production. In the comparison of various types of biological hydrogen production, ...

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Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a method for preparing a mixed bacterial flora for photosynthetic hydrogen production from biomass, which solves the problem that the hydrogen production efficiency of the existing photofermentation hydrogen production technology is low, the effect of photofermentation hydrogen production is not obvious, and High cost and lack of application prospects

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