Enterobacter cloacae for degrading petroleum pollution and application thereof

A technology of Enterobacter cloacae and oil pollution, applied in the restoration of contaminated soil, bacteria, and microbial-based methods, etc., can solve environmental problems, human health damage, carcinogenesis and mutagenesis, etc.

Active Publication Date: 2020-05-15
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the main components of petroleum are alkane, benzene, toluene, xylene and other complex aromatic hydrocarbons, these substances are highly toxic, some have carcinogenic and mutagenic effects, and are difficult to remove after entering the soil, and will enter the surrounding watershed with runoff, Groundwater, oil fields and the surrounding ecological environment have brought serious environmental problems
Harmful petroleum pollutants are enriched in the human body through the food chain, and will also cause serious damage to human health
[0004] In the 20th century, soil pollution caused by petroleum was limited to physical and chemical methods. The former destroyed most organic pollutants through incineration, which was expensive and not conducive to treatment; the latter used chemical extraction, which had better results. However, it is restricted because it is easy to cause secondary pollution

Method used

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  • Enterobacter cloacae for degrading petroleum pollution and application thereof
  • Enterobacter cloacae for degrading petroleum pollution and application thereof
  • Enterobacter cloacae for degrading petroleum pollution and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 The acquisition and identification of Enterobacter cloacae (Enterobacter cloacae) 2#

[0018] (1) Screening of Enterobacter cloacae 2#

[0019] Soil samples were collected in the soil environment near an oil well in Panjin, Liaoning, and 10g of soil samples were weighed, put into a triangular flask filled with 90mL of sterile water and glass beads, and shaken at a speed of 180r / min for 20h at room temperature , stand still after shaking. After an obvious solid-liquid interface appears, take the soil suspension and use the gradient dilution method for 10 -1 、10 -2 、10 -3 Gradient concentration dilution.

[0020] The supernatants with different concentration gradients were added to the petroleum inorganic salt medium containing petroleum for enrichment, cultured in a constant temperature shaker at 37°C and 120r / min for 7 days, and cycled 3 times.

[0021] Petroleum inorganic salt medium composition: K 2 HPO 4 6g, KH 2 PO 4 6g, (NH 4 ) 2 SO 4 6g, N...

Embodiment 2

[0044] Example 2 Application of Enterobacter cloacae (Enterobacter cloacae) 2# in degrading petroleum pollution

[0045] (1) Culture of Enterobacter cloacae 2#

[0046] Medium formula: beef extract 3g, peptone 10g, NaCl 5g, agar 15-20g, pH value 7.0-7.2, distilled water 1000mL.

[0047]Put 100mL prepared medium into a 250mL Erlenmeyer flask, sterilize at 121°C for 30min, cool at room temperature, inoculate 2 rings of Enterobacter cloacae (Enterobacter cloacae) 2# into the Erlenmeyer flask, and then put it into a constant temperature shaker to vibrate , adjust the rotation speed to 180r / min, and the temperature to 30°C.

[0048] (2) Effect of Enterobacter cloacae 2# on oil degradation

[0049] Petroleum inorganic salt medium composition: K 2 HPO 4 6g, KH 2 PO 4 6g, (NH 4 ) 2 SO 4 6g, NaCl 5g, petroleum 1g, distilled water 1L, pH7.0, sterilized at 121°C for 20min.

[0050] According to volume percentage, add respectively 1%, 3%, 5%, 7%, 10% Enterobacter cloacae (Ent...

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Abstract

The invention relates to enterobacter cloacae for degrading petroleum pollution and application thereof. The enterobacter cloacae 2# provided by the invention has a preservation number of CGMCC No. 18937, and the strain (enterobacter cloacae 2#) has an effect of degrading petroleum. The strain can be applied to the treatment of petroleum pollution, and the strain can be removed through natural degradation after the petroleum is degraded by the strain, so that the strain can be used for the treatment of the petroleum-polluted environment without causing secondary pollution to the environment.

Description

technical field [0001] The invention relates to a bacterial strain screened out from petroleum-contaminated soil and capable of degrading petroleum. [0002] The Enterobacter cloacae referred to in the present invention, named: Enterobacter cloacae (Enterobacter cloacae) 2#, has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on November 11, 2019, and the preservation number is CGMCC No.18937 . Background technique [0003] With the development of my country's petroleum industry, in the process of petroleum production, transportation and use, due to improper operation or accidents, it will cause serious pollution to the environment. In recent years, the area of ​​land polluted by oil has been expanding, and the degree of pollution has become increasingly serious. Since the main components of petroleum are alkane, benzene, toluene, xylene and other complex aromatic hydrocarbons, these substances are highly t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCC12N1/20B09C1/10C12R2001/01C12N1/205
Inventor 付保荣嫣雨朦杨丰硕王晞懿郭宏伟刘宇张润洁
Owner LIAONING UNIVERSITY
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