Method for enhancing colonization of enterobacter cloacae Y16 in plant root system

A technology of Enterobacter cloacae and plant root system, applied in chemical instruments and methods, fertilizer mixtures, organic fertilizers, etc., can solve the problems of insufficient colonization ability, affecting cadmium absorption of heavy metal hyperaccumulation plants, etc., and achieves increasing biofilm area, The effect of improving repair efficiency

Inactive Publication Date: 2021-05-14
JINAN UNIVERSITY
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the technical defect that the existing Enterobacter cloacae Y16 colonizes the root system of plants insufficiently, which affects the absorption of cadmium in the soil by heavy metal hyperaccumulator plants, and provides a method for strengthening the colonization of Enterobacter cloacae Y16 in the root system of plants

Method used

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  • Method for enhancing colonization of enterobacter cloacae Y16 in plant root system
  • Method for enhancing colonization of enterobacter cloacae Y16 in plant root system
  • Method for enhancing colonization of enterobacter cloacae Y16 in plant root system

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Embodiment 1

[0031] Embodiment 1 adds low-molecular-weight organic acid to the influence of nightshade nightshade biomass

[0032] The pot design includes siderophore bacteria (EC), Pseudomonas fluorescens (GI), three low-molecular organic acids (MA, CA and SA) and no bacteria (CK), a total of 15 treatments, each treatment 3 in parallel, a total of 45 pots. Adopt the flower pot that diameter is 18cm, every pot is equipped with 1.5kg farmland soil (total cadmium content is 1.087mg / kg, pH value is 7.26, and available phosphorus concentration is 38.54mg / kg), regulates about 80% of water holding capacity in the pot. Preparation of bacterial suspension: inoculate EC and GI bacteria into LB liquid medium from a slant, 28°C, 150r min -1 Cultivate on a shaker for 14h, 4 000r·min -1 Centrifuge for 10 minutes to collect the bacteria, resuspend with an equal volume of sterile water for later use (the concentration of the bacteria solution is 10 8 CFU / mL), and a certain concentration of low-molecul...

Embodiment 2

[0034] Example 2 Effects of Different Small Molecular Organic Acids and EC or GI Bacteria on Solanum Solanum Cd Accumulation

[0035] Harvest the aerial part of Solanum nigrum, place it in an oven for 0.5h at 105°C, and then completely dry it at 60°C. After digestion with concentrated nitric acid, the concentration of heavy metals in the solution was measured, and the accumulation of cadmium in Solanum nigrum was obtained through conversion. Such as image 3 The results showed that, compared with the group inoculated with EC alone, exogenous addition of 50 μmol / L low-molecular organic acid could increase the cadmium accumulation of Solanum solanum, among which the cadmium accumulation of EC+CA and EC+SA increased most significantly, followed by EC+CA (+30.47%)>EC+SA (+21.15%)>EC+MA (+6.54%). However, the cadmium accumulation in the GI group was not increased by the addition of low-molecular organic acids (such as Figure 4 Shown), on the contrary, compared with adding GI bact...

Embodiment 3

[0036] The colonization of embodiment 3EC or GI bacterium in Solanum nigrum root system

[0037] The colonization and distribution of green fluorescent protein-labeled EC bacteria in the root system of Solanum nigrum were observed by laser confocal microscopy. Such as Figure 7 It was shown that, compared with the EC group alone, exogenous addition of 50 μmol / L low-molecular organic acid can promote the formation of stable and sheet-like biofilms of Enterobacter cloacae Y16 in the root system of Solanum nigrum. It was confirmed that specific low-molecular-weight organic acids strengthened the biofilm formation of EC bacteria, thereby enhancing their colonization ability in plant roots.

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Abstract

The invention discloses a method for enhancing colonization of enterobacter cloacae Y16 in a plant root system. Research finds that when bacterial liquid of enterobacter cloacae Y16 and low-molecular organic acid are jointly applied to the rhizosphere soil of a heavy metal hyperaccumulation plant, the formation of a biological membrane of the enterobacter cloacae in the plant root system can be remarkably promoted, the colonization ability of the enterobacter cloacae is enhanced, the growth of the plant is promoted, and the efficiency of microorganism-plant combined remediation of the farmland soil polluted by medium-low-concentration cadmium is improved. The low-molecular organic acid is at least one of malic acid, citric acid or succinic acid which has a directional recruitment effect on the enterobacter cloacae Y16. According to the method, the remediation efficiency of the heavy metal hyperaccumulation plant on the medium-low heavy metal polluted farmland soil can be remarkably improved.

Description

technical field [0001] The present invention relates to the field of biotechnology, and more specifically, relates to a method of strengthening the colonization of Enterobacter cloacae Y16, a rhizosphere growth-promoting bacterium with cadmium activation, in the root system of Solanum nigrum, a cadmium superaccumulative plant, to promote plant growth, and to improve microbial- Efficiency of phytocombined remediation of medium and low concentrations of cadmium-contaminated farmland soil. Background technique [0002] In practical application, phytoremediation is often affected by factors such as soil physical and chemical properties, heavy metal occurrence forms, and rhizosphere microorganisms, which lead to slow plant growth, small biomass, and low remediation efficiency of heavy metals in soil. Rhizosphere growth-promoting bacteria, as the most commonly used type of microorganisms for strengthening plants to accumulate heavy metals in soil, have strong application potential...

Claims

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Application Information

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IPC IPC(8): C09K17/14C09K101/00
CPCC09K17/14C09K2101/00
Inventor 王立立唐万鹏王东升李取生何宝燕
Owner JINAN UNIVERSITY
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