The invention relates to a breathable knitted spacer fabric friction generator and a preparation method thereof. The method comprises the steps of weaving a top layer and a bottom layer by using a three-wire plating process, controlling the yarn angles of surface yarns, intermediate yarns and ground yarns respectively such that the three yarns are sequentially arranged according to a certain positional relationship and then are woven, at the same time, in the weaving process, performing alternate tucking of spacer wires between a top layer ground yarn and a bottom layer ground yarn with a certain number of spacing stitches to form the breathable knitted spacer fabric friction generator, wherein a top layer surface yarn and a bottom layer surface yarn are at the outmost sides of a compositelayer and are insulating yarns, a top layer intermediate yarn and a bottom layer intermediate yarn are conductive yarns, the top layer ground yarn and the bottom layer ground yarn are opposite and are a triboelectric negative yarn and a triboelectric positive yarn correspondingly, and a spacer layer is composed of a set of spacer wires that are simultaneously connected to the top layer ground yarn and bottom layer ground yarn. The preparation method of the invention has the advantages of simple process and high weaving efficiency, and the obtained knitted spacer fabric friction generator hasgood gas permeability and has excellent promotion value.