69 results about "Cowpox virus" patented technology

The present invention relates to the identification of gene sequences and proteins involved in vaccinia virus dominant T cell epitopes. Two vaccinia virus CD8+ T cell epitopes restricted by the most common human MHC class I allele, HLA-A0201 have been identified. Both epitopes are highly conserved in vaccinia and variola viruses. The induction of the T cell responses following primary vaccination is demonstrated by the kinetics of epitope specific CD8+ T cells in 3 HLA-A0201 individuals. This information will be useful for the design and analyses of the immunogenicity of experimental vaccinia vaccines, and for basic studies of human T cell memory.

Embodiments of the invention are directed methods that include a thymidine kinase deficient vaccinia virus. The methods include administering the vaccinia virus at increased viral concentrations. Further aspects of the invention include methods for inducing oncolysis or collapse of tumor vasculature in a subject having a tumor comprising administering to a subject at least 1×108 infectious viral particles of a TK-deficient, GM-CSF-expressing, replication-competent vaccinia virus vector sufficient to induce oncolysis of cells in the tumor.

[Problem]To provide a set of virus vectors which can be used for producing a prime / boost vaccine that can activate both cellular immunity and humoral immunity and is effective on infections by pathogenic microorganisms and malignant tumors which are generally believed to be difficult to be treated by vaccine therapy.[Solution]Provided is a set of virus vectors for prime / boost vaccines, comprising the following virus vector (a) and virus vector (b): (a) a vaccinia virus vector which carries a gene encoding an immunogenic polypeptide in such a manner that the gene can be expressed; and (b) a Sendal virus vector which carries the gene encoding the immunogenic polypeptide in such a manner that the gene can be expressed.

The present invention relates to recombinant modified vaccinia Ankara (MVA) virus containing restructured sites useful for the integration of heterologous nucleic acid sequences into an intergenic region (IGR) of the virus genome, where the IGR is located between two adjacent, essential open reading frames (ORFs) of the vaccinia virus genome, wherein the adjacent essential ORFs are non-adjacent in a parental MVA virus used to construct the recombinant MVA virus, and to related nucleic acid constructs useful for inserting heterologous DNA into the genome of a vaccinia virus, and further to the use of the disclosed viruses as a medicine or vaccine.

The present invention relates to methods for purification of Vaccinia viruses (VV) and / or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production.

The compositions and methods are described for generating an immune response to an antigen. The compositions and methods described herein relate to a modified vaccinia Ankara (MVA) vector encoding one or more viral antigens as a fusion product with a viral glycoprotein and matrix protein for generating a protective immune response to a subject to which the vector is administered. The compositions and methods of the present invention are useful both prophylactically and therapeutically and may be used to prevent and / or treat diseases

The technology of the present disclosure relates to the use of Heat-inactivated modified vaccinia Ankara (MVA) virus (Heat-iMVA) or Heat-inactivated vaccinia virus as a vaccine immune adjuvant. In particular, the present technology relates to the use of Heat-iMVA as a vaccine adjuvant for tumor antigens in cancer vaccines alone or in combination with immune checkpoint blockade (ICB) antibodies foruse as a cancer immunotherapeutic.

The present invention discloses recombinant vaccinia virus (VV) virions that are resistant to antiviral defenses and have enhanced anti-tumor activities. In one embodiment, the recombinant VV comprise one or more variant VV proteins that have mutations at one or more neutralizing antibody epitopes, thereby conferring viral escape from the neutralizing antibodies. In another embodiment, the recombinant VV is resistant to complement-mediated neutralization due to the expression of a regulator of complement activation (e.g. CD55). In another embodiment, the recombinant VV has enhanced anti-tumor activities due to the expression of bi-specific antibodies co-targeting cancer cells and immune effector cells, or the expression of a polypeptide blocking the PD-1 pathway. The recombinant vaccinia virus virions can be used to treat cancer in a subject.

The present invention provides compositions and methods for generation of premature transcription termination products. Premature termination of transcription interferes with the normal transcription and termination of early phase genes of the poxviruses and results in inhibition of replication of the poxviruses. The compositions of the present invention are oligonucleotides comprising a U5NU sequence flanked by ribonucleotides, deoxyribonucleotides or modifications thereof. This invention also provides methods for inhibition of replication of poxvirus including the small poxvirus, Monkeypox virus and vaccinia virus.

A recombinant vaccinia virus derived from the vaccinia virus Ankara (MVA) encoding and capable of expressing the E2 gene of Bovine papillomavirus. Also, the use of the virus in the treatment of lesions caused by papillomavirus.

The present invention relates to an oncolytic virus improved in safety and anticancer effect and a use thereof. The oncolytic virus improved in safety and anticancer effect of the present invention isobtained by inserting an HSV-TK fragment-encoding gene into a TK gene region to delete TK of Vaccinia virus. In addition, the oncolytic virus of the present invention expresses an HSV-TK fragment tophosphorylate GCV so that cancer cells infected with the oncolytic virus and even their neighboring cancer cells can be killed. In addition, GCV is also involved in the suppression of viral proliferation and thus can control side effects caused by a virus even upon the administration of a high dose of the virus. Furthermore, an anticancer effect is increased even though the number of viral particles is reduced due to the suppression of GCV against virus proliferation. Therefore, the oncolytic virus improved in safety and anticancer effect of the present invention can be effectively used for the treatment of cancer.

The invention relates to the field of vaccines, and discloses a protein vaccinia vaccine and a method for detecting its effectiveness. The protein vaccine contains viral recombinant protein D8L expressed by yeast and with a purity >90% of endotoxin removed. The preparation method is as follows: expressing yeast with a purity >90% 90% of the recombinant protein D8L of vaccinia virus has been treated to remove endotoxin to obtain a recombinant protein D8L solution with an endotoxin content <5 EU / ml. Efficacy testing method is as follows: the recombinant protein D8L solution that removes endotoxin is injected subcutaneously twice to immunize mice. After 21 days, specific antibodies and neutralizing antibodies can be detected in the mouse serum, indicating that the mice have acquired anti-vaccinia virus immunity. immune protection. The protein vaccine of the present invention does not require additional vaccine adjuvants, and can theoretically replace traditional live vaccinia virus vaccines, and play a role in resisting pox virus infections such as smallpox virus.

Expression vectors ideal for use in vaccinating individuals against disease based on vaccinia virus and other chordopoxviruses having high expression of recombinant genes and low expression of vector genes in target animals, and low expression of recombinant genes and high expression of vector genes in cells used for propagation.

The present invention relates to a novel Modified Vaccinia Ankara (MVA) related virus. The present invention also relates to a method for culturing said MVA related virus and to a method for producing said MVA related virus. Further, the present invention relates to a pharmaceutical composition comprising said MVA related virus and one or more pharmaceutical acceptable excipient(s), diluent(s), and / or carrier(s). Furthermore, the present invention relates to a vaccine comprising said MVA related virus. In addition, the present invention relates to said MVA related virus for use in medicine.

There is provided a method of treating chronic hepatitis B infection (CHB) in a human, comprising the steps of: a) administering to the human a composition comprising a replication-defective chimpanzee adenoviral (ChAd) vector comprising a polynucleotide encoding a hepatitis B surface antigen (HBs) and a nucleic acid encoding a hepatitis B virus core antigen (HBc); b) administering to the human acomposition comprising a Modified Vaccinia Virus Ankara (MVA) vector comprising a polynucleotide encoding a hepatitis B surface antigen (HBs) and a nucleic acid encoding a hepatitis B virus core antigen (HBc); and c) administering to the human a composition comprising a recombinant hepatitis B surface antigen (HBs), recombinant hepatitis B virus core antigen (HBc) and an adjuvant.