36 results about "Cellulomonas flavigena" patented technology

The invention discloses a straw degradation acidification microbial inoculum and a manufacturing method thereof. Active ingredients of the composite microbial inoculum for degrading straw are cellulomonas flavigena, clostridium cellulolyticum, bacillus subtilis and bacillus cereus. The microbial inoculum breaks through the limitation that fungi and lytic enzymes thereof cannot quickly and efficiently decompose natural cellulose, a key pretreatment technology is provided for straw biogas fermentation, and the straw degradation acidification microbial inoculum has wide application prospect in the field of preparation of biogas from lignocellulose raw materials such as the straw.

The invention discloses a straw decomposing inoculant and a method for fast decomposing straws. The decomposing inoculant comprises a chemical curing agent and a biological curing agent, wherein the chemical curing agent is one or a mixture of more than two of ammonium bicarbonate, alkali lignin and lignin sodium sulfonate; the biological curing agent is a bactericide containing cellulose-decomposing bacteria and an organic matrix, wherein the bactericide containing cellulose-decomposing bacteria is composite bacteria containing more than two of Bacillus licheniformis, Bacillus cereus, Saccharomyces cerevisiae, Candida, Bacillus subtilis, Cellulomonas flavigena, Streptomyces microflavus and Trichoderma viride. The method for decomposing straws comprises the following steps of: crushing the straws, adding water solution of the chemical curing agent, and then adding the biological curing agent. The method of the invention has simple and easy operation, easily obtained materials and lower cost, shortens the decomposing time, enables the straws to be decomposed within 7-10 days, and has excellent popularization value.

The invention discloses a combined remediation method for radioactive contaminated soil. The combined remediation method comprises the following remediation steps: step 1, pre-oxidizing ozone; step 2, adding a composite additive which comprises nano titanium dioxide, cerium oxide, molybdenum oxide, boric acid, silica gel, diatomite, ammonium molybdate, nano manganese dioxide, zinc oxide and ferrous oxide, turning over, carrying out UV-irradiation, alternatively turning over once, shading ultraviolet light, and placing while ventilating at the room temperature; step 3, spraying a microbial strain liquid which comprises staphylococcus pasteuri, clostridium butyricum and cellulomonas flavigena, and controlling soil humidity and a soil temperature; and step 4, adding a compound fertilizer, turning over, planting clovers and pennisetum sinese seedlings, and harvesting the grown-up clovers and pennisetum sinese seedlings. The combined remediation method provided by the invention is good in remediation effect on cesium and iodine radioactive contaminated soil. Cesium and iodine radioactive contaminants of the remediated soil are greatly reduced, so that remediation and control on large-area radioactive contaminated soil can be met.

The invention discloses a method for removing fluoroquinolones antibiotics in poultry and livestock excrement. The method includes the steps of 1, preparing compost raw materials, wherein chicken excrement, sawdust, sterile distilled water and high-temperature-resistant fungicide are prepared into the compost raw materials, the mass ratio of the chicken excrement to the sawdust is 5-10 to 1, the water content of the compost raw materials ranges from 55% to 60%, and the high-temperature-resistant fungicide is cellulomonas flavigena; 2, carrying out high-temperature composting, wherein the pile overturning frequency is controlled to enable the compost temperature to range from 45 DEG C to 65 DEG C, and high-temperature fermentation is achieved; 3, carrying out intermediate-temperature composting, wherein after high-temperature fermentation, the manual pile overturning frequency is controlled to enable the temperature of a compost product to range from 30 DEG C to 45 DEG C, and intermediate-temperature fermentation is achieved. According to the method, the fluoroquinolones antibiotics in the poultry and livestock excrement are removed sufficiently through the degradation effect of high-temperature-resistant strains and relative microorganisms, and therefore the fluoroquinolones antibiotics in organic fertilizers are reduced to the safe level.

The invention provides a preparation method of a fertilizer making agent. The preparation method includes the following steps that firstly, Cytophaga fermentans ATCC 19072, Cellulomonas flavigena ATCC 482, Butyrivibrio fibrisolvens ATCC 19171 and Clostridium papyrosolvens ATCC 3541 are inoculated to a culture medium with cellulose to be cultured according to the thallus number ratio of 3-17:4-25:5-10:15-28; secondly, bacillus megatherium ACCC 01742, candida tropicalis ACCC 20199 and phanerochaete chrysosporium ACCC 30530 are inoculated to a PDA culture medium to be cultured in a mixed mode according to the thallus number ratio of 5-14:15-20:8-20; thirdly, culture obtained from the first step and the second step is evenly mixed according to the volume ratio of 1:1 and centrifuged, sediment is removed, supernate is taken, and then the fertilizer making agent is obtained. The invention further discloses the fertilizer making agent prepared through the method and application of the fertilizer making agent in preparing edible fungus compost. The fertilizer efficiency of the compost can be remarkably improved, the fertilizer making agent is economical and environmentally friendly, and no influence is generated on growth of crops and soil.

The invention discloses accelerant for root growth of taxus chinensis and a method for applying the accelerant to crossbreeding. The accelerant comprises candida yeast fermentation liquid, bacillus licheniformis fermention liquid, cellulomonas flavigena fermentation liquid, dung producing alkali coli fermention liquid, gibberellins, activated carbon and dipotassium phosphate. The accelerant can push taxus chinensis tissue culture seedlings to root rapidly and raise the survival rate, and the accelerant has significant and profound significance to deep development and wide applciaiton of taxus plant resources and wide is application prospect.

The invention belongs to the technical field of microorganisms and discloses a microbial preparation for repairing hydroxymethyl cellulose wastewater. The microbial preparation is prepared from the following raw materials: anabaena variabilis domestication liquid, cellulomonas flavigena domestication liquid, bacillus subtilis domestication liquid and Aspergillus niger domestication liquid, wherein the anabaena variabilis domestication liquid, the cellulomonas flavigena domestication liquid, the bacillus subtilis domestication liquid and the Aspergillus niger domestication liquid are separately used. According to the microbial preparation disclosed by the invention, four microorganisms are adopted, reasonably matched and well synergized, so that proliferation and enzyme activity of the microorganisms can be promoted; the microbial preparation has a good effect for repairing hydroxymethyl cellulose wastewater.

The invention relates to the biological fermentation technology, in particular to a compound microbial starter, and a method which uses the compound microbial starter to produce high-energy protein biological feed. The compound microbial starter selects cellulomonas sp dinitrogen, cellulomonas flavigena, condensate bacillus, lactobacillus acidophilus, bacillus subtilis, lactobacillus starch, compound enzyme bacteria, propionic acid bacteria, candida yeast, saccharomyces diastaticus, saccharomyces cerevisiae, as well as aroma-producing yeast as the bacteria sources, and makes use of the modern biotechnology to prepare various microbial agents; the microbial agents are then treated with anaerobic fermentation with the discarded sweet corn cobs, corn stalks, pea skins, abandoned vegetables and other discarded crops, so as to obtain the high-energy protein biological feed required in livestock and poultry breeding industry. The invention solves the problem of low utilization rate of crop resources; and the high-energy protein biological feed can improve the palatability of feed, increase the enzyme content of the feed, provide an effective way for developing the animal husbandry and solving the food conflicts between people and livestock, as well as reduce the pollution to the environment caused by the traditional crop waste burning.