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36 results about "Cellulomonas flavigena" patented technology

Manifold microbe mixed culture fermentation agent and method for producing high energy protein biology feedstuff

The invention relates to the biological fermentation technology, in particular to a compound microbial starter, and a method which uses the compound microbial starter to produce high-energy protein biological feed. The compound microbial starter selects cellulomonas sp dinitrogen, cellulomonas flavigena, condensate bacillus, lactobacillus acidophilus, bacillus subtilis, lactobacillus starch, compound enzyme bacteria, propionic acid bacteria, candida yeast, saccharomyces diastaticus, saccharomyces cerevisiae, as well as aroma-producing yeast as the bacteria sources, and makes use of the modern biotechnology to prepare various microbial agents; the microbial agents are then treated with anaerobic fermentation with the discarded sweet corn cobs, corn stalks, pea skins, abandoned vegetables and other discarded crops, so as to obtain the high-energy protein biological feed required in livestock and poultry breeding industry. The invention solves the problem of low utilization rate of crop resources; and the high-energy protein biological feed can improve the palatability of feed, increase the enzyme content of the feed, provide an effective way for developing the animal husbandry and solving the food conflicts between people and livestock, as well as reduce the pollution to the environment caused by the traditional crop waste burning.
Owner:TANGSHAN SENA BIOLOGY TECH SERVICING

Method for removing fluoroquinolones antibiotics in poultry and livestock excrement

The invention discloses a method for removing fluoroquinolones antibiotics in poultry and livestock excrement. The method includes the steps of 1, preparing compost raw materials, wherein chicken excrement, sawdust, sterile distilled water and high-temperature-resistant fungicide are prepared into the compost raw materials, the mass ratio of the chicken excrement to the sawdust is 5-10 to 1, the water content of the compost raw materials ranges from 55% to 60%, and the high-temperature-resistant fungicide is cellulomonas flavigena; 2, carrying out high-temperature composting, wherein the pile overturning frequency is controlled to enable the compost temperature to range from 45 DEG C to 65 DEG C, and high-temperature fermentation is achieved; 3, carrying out intermediate-temperature composting, wherein after high-temperature fermentation, the manual pile overturning frequency is controlled to enable the temperature of a compost product to range from 30 DEG C to 45 DEG C, and intermediate-temperature fermentation is achieved. According to the method, the fluoroquinolones antibiotics in the poultry and livestock excrement are removed sufficiently through the degradation effect of high-temperature-resistant strains and relative microorganisms, and therefore the fluoroquinolones antibiotics in organic fertilizers are reduced to the safe level.
Owner:CHINESE RES ACAD OF ENVIRONMENTAL SCI

Microbial agent for sewage treatment, attachment carrier of microbial agent and preparation method of microbial agent

The invention discloses a microbial agent for sewage treatment. The microbial agent comprises, by weight, 8-10 parts of clostridium acetobutylicum, 12-16 parts of bacillus licheniformis, 16-20 parts of bacillus subtilis, 5-7 parts of lactobacillus acidophilus, 5-7 parts of methanosarcina, 3-5 parts of methanosaeta, 6-8 parts of rhodopseudomonas palustris, 4-6 parts of cellulomonas flavigena, 8-10parts of streptococcus thermophilus, 8-10 parts of alcaligenes faecalis, 3-5 parts of bacillus amyloliquefaciens, 2-4 parts of bacillus nitrosomonas, 1-3 parts of issatchenkia orientalis and 3-5 partsof streptomyces bullis. Besides, the invention further discloses an attachment carrier of the microbial agent and a preparation method of the microbial agent. The microbial agent can be effectively attached to the carrier and accordingly has better activity. The microbial agent is reasonable in strain preparation and can be effectively attached to the attachment carrier to treat sewage, and the method for sewage treatment is simple, good in treatment effect and worthy of popularization.
Owner:ANJIEYU BEIJING OILFIELD TECHNICAL SERVICES CO LTD

Kitchen garbage biological fermentation microbial inoculum and application thereof

The invention relates to kitchen garbage biological fermentation microbial inoculum. The kitchen garbage biological fermentation microbial inoculum consists of four or more of bacillus subtilis, bacillus licheniformis, cellulomonas flavigena, pseudomonas pesodoalcaligenes, rhodop seudanonas palustris, frankiaceae actinomycesbovis, debaryomyces hansenii, and candida tropicalis and is loaded to a carrier consisting of one or two of wheat bran, saw dust or straw, and is subjected to freeze drying to obtain the microbial inoculum; and the microbial inoculum is mixed with kitchen garbage, and a fermentation reaction is performed under 50-65 DEG C, so that the kitchen garbage can be reduced by 70% within 6-10 hours, and the content of generated organic matter exceeds 90%. The kitchen garbage biological fermentation microbial inoculum has the advantages that the technical method can efficiently reduce kitchen garbage, the treated substances can be used for soil modification, and the kitchen garbage biological fermentation microbial inoculum has remarkable economic, social and environmental benefits.
Owner:NANKAI UNIV +1

Preparation process of carbon method lime sludge fermenting bacteria soil for improving acid soil

The invention belongs to the utilization of lime sludge in sugar manufacturing by a carbon method. The process comprises the following steps of: casting molasses alcohol waste mash to an excrement alcaligene bacteria liquid according to the inoculum size which is 0.5% of waste mash liquid, introducing air to ferment, wherein the PH value of the excrement alcaligene fermenting waste mash reaches about 5; further, mixing Cellulomonas flavigena bacteria liquid to fresh bagasse according to the inoculum size which is 0.5% of the fresh bagasse, and uniformly mixing; then, mixing the waste mash after fermentation of excrement alcaligene bacteria according to 10% of sludge amount, the inoculated bagasse according to 30% of sludge amount with lime sludge; uniformly mixing, composting to ferment, controlling the fermenting temperature at about 40 DEG C; and when the pH value of the mixed bacteria soil reaches about 8, obtaining the carbon method lime sludge fermenting bacteria soil. The bacteria soil is applied to red soil, so that red soil is loosened, and rhizospheric microorganisms of crops increase actively so as to accelerate growth of root systems of the crops and stem leaves. The bacteria soil solves the problem that red soil is hardened due to the lime sludge generated by sugar manufacturing by the carbon method applied to the acidic red soil, and the production of crops is increased.
Owner:YUNNAN KANGTAI ENVIRONMENT SCI & TECH

Culture medium for morchella esculenta cultivated species as well as preparation method and application of culture medium

The invention relates to a culture medium for morchella esculenta cultivated species as well as a preparation method and an application of the culture medium. The culture medium of the formula comprises components in parts by weight as follows: 40-60 parts of sorghum grains, 30-50 parts of straw, 10-15 parts of mushroom bran and 1-2 parts of gypsum powder; the raw materials are mixed uniformly, isometric cellulomonas flavigena and actinomyces roseus strains accounting for 1% of dry weight of the culture medium are added, water is added, water content is regulated to be 58%-65%, and stocking fermentation is executed; compost is subjected to steam sterilization and bottled, liquid strains or solid strains of morchella esculenta are inoculated for culturing, temperature of a culture room is 20-25 DEG C, the culture room is away from light, air humidity is 60%-65%, the culture room is ventilated twice every day, each ventilation lasts for 30 min, and culturing time is 10-25 d. The culturemedium has the characteristics of comprehensive nutrition, low cost, rapid fungus growth and strong hyphae, integrated development of production technology of the morchella esculenta is promoted, large-scale popularization can be realized, and the benefit is remarkable.
Owner:LIAONING ACAD OF AGRI SCI

Preparation and use method of thermophilic microbial agent for drying leather sludge

The invention relates to the technical field of fermentation biology, in particular to a preparation and use method of a thermophilic microbial agent for drying leather sludge. According to the invention, scientific and reasonable mixing and compounding are carried out according to the functions of thermophilic microorganisms such as streptococcus thermophilus, bacillus coagulans, lactobacillus acidophilus, cellulomonas flavigena, candida utilis, rhizopus oryzae and trichoderma viride, so that the thermophilic microorganism mixed flora is in an optimal growth state in the biological drying process of leather sludge, and the strains are mutually coordinated and not mutually antagonized. The microbial agent effectively inhibits the growth of harmful microorganisms, greatly improves the degradation efficiency and the deodorization effect of the leather sludge, greatly shortens the sludge drying time, remarkably reduces the water content of the leather sludge, has good sludge decrement effect, and has the advantages of high removal rate of sulfur and ammonia nitrogen, simple drying process, no toxicity and corrosion, convenient operation, small production investment, low production cost, no secondary pollution and stable sludge decrement.
Owner:湖南洁源生物能源科技有限公司

Efficient composite organic matter decomposing inoculant

InactiveCN102757916ABreak the drawbacks of traditional fermentation that takes more than 3 monthsBreaking the drawbacks that take more than 3 monthsBio-organic fraction processingBacteriaBiotechnologyDecomposition
The invention discloses an efficient composite organic matter decomposing inoculant capable of accelerating decomposition of organic matters, belonging to the technical field of microbes. The efficient composite organic matter decomposing inoculant is prepared by mixing the following components in percentage by volume: 30% of Cellulomonas flavigena zymophyte solution, 30% of Bacillus stearothermophilus zymophyte solution and 40% of Bacillus subtilis zymophyte solution. The total viable count of the three strains is 1 billion / ml, and the microbial inoculant type is liquid. The strains are respectively cultured in a fermentation tank without foreign microbes, and then compounded to obtain the efficient composite organic matter decomposing inoculant. By matching the three strains, various organic matters can be quickly degraded and converted into available organic fertilizers containing multifunctional probiotics within 15 days or so, and thus, the invention overcomes the defect that the traditional degradation needs more than 3 months.
Owner:XUZHOU QIYUAN ECOLOGY AGRI DEV

Method for extracting tea saponin from tea seed meal with microbial fermentation method

The invention relates to a method for extracting tea saponin from tea seed meal with a microbial fermentation method. The method is characterized by comprising steps as follows: taking crushed dry tea seed meal as a raw material, taking water as a solvent, adding a proper amount of growth factors (NH4)2SO4, KH2PO4 and K2HPO4, taking Aspergillus niger, Cellulomonas flavigena, saccharomycetes and bacillus subtilis which are sequentially added as fermentation media, preparing a tea saponin coarse extract through multiple times of fermentation treatment, and finally, purifying the extract with a macroporous resin method to obtain the tea saponin with the purity higher than 85%.
Owner:SHANGHAI OCEAN UNIV

Method for evaluating microbial powder promoting degradation of garden waste

The invention discloses a method for evaluating microbial powder promoting degradation of garden waste. In the method, the microbial powder is inoculated to the landscaping waste, culture is performedat the constant temperature of 30+ / -1 DEG C, the mass change of degradation residuums of the landscaping waste is periodically measured, and the degradation efficiency of the microbial powder to thelandscaping waste is evaluated based on the degradation weight loss rate; when culture is performed for 15 days, the degradation weight loss rate of the microbial powder to the landscaping waste is not lower than 30%; the microbial powder is formed by mixing cellulomonas flavigena thalli, an adsorptive carrier and a protective agent in the mass ratio of 1:(0.5-1.5):(0.5-1.5). The adsorptive carrier is selected from dextrin, diatomaceous earth, talcum powder and calcium carbonate; a wetting agent is selected from Tween 80, polyethylene glycol, soluble starch and sodium lauryl sulfate; the protective agent is selected from turf, kaolin and sodium alginate or sodium carboxymethyl cellulose. The microbial powder is used for treating the landscaping waste and can meet the requirement for large-scale composting disposal of the landscaping waste.
Owner:XIAN BOTANICAL GARDEN SHAANXI PROV

A method for removing fluoroquinolone antibiotics in poultry feces

The invention discloses a method for removing fluoroquinolones antibiotics in poultry and livestock excrement. The method includes the steps of 1, preparing compost raw materials, wherein chicken excrement, sawdust, sterile distilled water and high-temperature-resistant fungicide are prepared into the compost raw materials, the mass ratio of the chicken excrement to the sawdust is 5-10 to 1, the water content of the compost raw materials ranges from 55% to 60%, and the high-temperature-resistant fungicide is cellulomonas flavigena; 2, carrying out high-temperature composting, wherein the pile overturning frequency is controlled to enable the compost temperature to range from 45 DEG C to 65 DEG C, and high-temperature fermentation is achieved; 3, carrying out intermediate-temperature composting, wherein after high-temperature fermentation, the manual pile overturning frequency is controlled to enable the temperature of a compost product to range from 30 DEG C to 45 DEG C, and intermediate-temperature fermentation is achieved. According to the method, the fluoroquinolones antibiotics in the poultry and livestock excrement are removed sufficiently through the degradation effect of high-temperature-resistant strains and relative microorganisms, and therefore the fluoroquinolones antibiotics in organic fertilizers are reduced to the safe level.
Owner:CHINESE RES ACAD OF ENVIRONMENTAL SCI

A kind of fertilizer making agent and its preparation method and application

The invention provides a preparation method of a fertilizer making agent. The preparation method includes the following steps that firstly, Cytophaga fermentans ATCC 19072, Cellulomonas flavigena ATCC 482, Butyrivibrio fibrisolvens ATCC 19171 and Clostridium papyrosolvens ATCC 3541 are inoculated to a culture medium with cellulose to be cultured according to the thallus number ratio of 3-17:4-25:5-10:15-28; secondly, bacillus megatherium ACCC 01742, candida tropicalis ACCC 20199 and phanerochaete chrysosporium ACCC 30530 are inoculated to a PDA culture medium to be cultured in a mixed mode according to the thallus number ratio of 5-14:15-20:8-20; thirdly, culture obtained from the first step and the second step is evenly mixed according to the volume ratio of 1:1 and centrifuged, sediment is removed, supernate is taken, and then the fertilizer making agent is obtained. The invention further discloses the fertilizer making agent prepared through the method and application of the fertilizer making agent in preparing edible fungus compost. The fertilizer efficiency of the compost can be remarkably improved, the fertilizer making agent is economical and environmentally friendly, and no influence is generated on growth of crops and soil.
Owner:BIOGAS SCI RES INST MIN OF AGRI

Manifold microbe mixed culture fermentation agent and method for producing high energy protein biology feedstuff

The invention relates to the biological fermentation technology, in particular to a compound microbial starter, and a method which uses the compound microbial starter to produce high-energy protein biological feed. The compound microbial starter selects cellulomonas sp dinitrogen, cellulomonas flavigena, condensate bacillus, lactobacillus acidophilus, bacillus subtilis, lactobacillus starch, compound enzyme bacteria, propionic acid bacteria, candida yeast, saccharomyces diastaticus, saccharomyces cerevisiae, as well as aroma-producing yeast as the bacteria sources, and makes use of the modern biotechnology to prepare various microbial agents; the microbial agents are then treated with anaerobic fermentation with the discarded sweet corn cobs, corn stalks, pea skins, abandoned vegetables and other discarded crops, so as to obtain the high-energy protein biological feed required in livestock and poultry breeding industry. The invention solves the problem of low utilization rate of crop resources; and the high-energy protein biological feed can improve the palatability of feed, increase the enzyme content of the feed, provide an effective way for developing the animal husbandry and solving the food conflicts between people and livestock, as well as reduce the pollution to the environment caused by the traditional crop waste burning.
Owner:TANGSHAN SENA BIOLOGY TECH SERVICING
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