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Method for evaluating microbial powder promoting degradation of garden waste

A technology of garden waste and microbial bacteria, which is applied in the field of application evaluation of microbial powder to promote the degradation of landscaping waste, can solve the problems that the key role is not clear, and the biological effectiveness of liquid bacteria has not been studied.

Pending Publication Date: 2019-06-28
XIAN BOTANICAL GARDEN SHAANXI PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the bacterial strain is still an equal proportion combination of various bacterial liquids. It is not clear which strain plays a key role and how to participate in the degradation process, and the bioavailability of the liquid bacterial strain has not been studied

Method used

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  • Method for evaluating microbial powder promoting degradation of garden waste
  • Method for evaluating microbial powder promoting degradation of garden waste
  • Method for evaluating microbial powder promoting degradation of garden waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] This embodiment provides a microbial powder capable of degrading garden cellulose, the effective viable bacteria of which are Cellulomonas flavigena which has a strong ability to degrade cellulose. The Cellulomonas lutea used in this example is a model strain of the genus Cellulomonas, which was purchased from the General Microbiology Center of China Committee for the Collection of Microbial Cultures. In order to prepare a solid microbial powder that is durable and convenient to use, in this example, the liquid fermented cells of Cellulomonas lutea were mixed with a specific ratio of suitable adsorptive carrier, wetting agent and protective agent. Specifically, the adsorptive carrier is selected from one or more of dextrin, diatomaceous earth, talcum powder or calcium carbonate, and in terms of mass ratio, the ratio of the flavin-producing cellulomonas to the adsorptive carrier is 1: (0.5-1.5); Wetting agent selects one or more in Tween 80, polyethylene glycol, soluble ...

Embodiment 2

[0046] This embodiment provides a preparation method of microbial bacterial powder that can degrade garden cellulose, including steps: 1) bacterial liquid fermentation, liquid fermentation of Cellulomonas xanthogenes, and production of Cellulomonas xanthomonas in the fermentation liquid when the fermentation is stopped The number of viable bacteria is not less than 2.0×10 8 pcs / mL; 2) Separation of cells, removal of water in the fermentation liquid to obtain Cellulomonas flavinogenes cells; 3) Preparation of bacteria powder, adding adsorption carrier, wetting agent and protective agent according to the mass ratio, stirring Mix evenly and dry in vacuum to obtain the microbial powder.

[0047] 1) Bacterial liquid fermentation

[0048] The number of effective viable bacteria reaches 2.0×10 8 Cellulomonas flavigena (Cellulomonas flavigena) culture fermentation broth of more than individual / mL is used as seed liquid, inoculates in the fermenter that liquid fermentation is used wi...

Embodiment 3

[0065] This embodiment provides the evaluation method of the microbial bacterium powder that embodiment 1 and 2 prepare. This embodiment uses the degradation weight loss rate method to evaluate the degradation efficiency of microbial bacterial powder to landscaping waste, and the microbial bacterial powder of different storage periods (30d, 90d, 150d, 210d, 270d, 330d, 390d and 450d) are respectively configured into 5 For ‰ bacterial powder suspension, calculate its degradation rate of landscaping waste on the 5th day, 10th day and 15th day of incubation at a constant temperature (30±1°C), and the greater the weight loss rate, the higher the degradation efficiency.

[0066] The landscaping waste described in this embodiment refers to one or more withered stems of reed (Phragmites australis), tulip (Tulipa gesneriana), silvergrass (Chloranthus japonicus), and iris (Iris tectorum). In order to avoid the pollution and interference of other bacteria on the selected materials, the ...

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Abstract

The invention discloses a method for evaluating microbial powder promoting degradation of garden waste. In the method, the microbial powder is inoculated to the landscaping waste, culture is performedat the constant temperature of 30+ / -1 DEG C, the mass change of degradation residuums of the landscaping waste is periodically measured, and the degradation efficiency of the microbial powder to thelandscaping waste is evaluated based on the degradation weight loss rate; when culture is performed for 15 days, the degradation weight loss rate of the microbial powder to the landscaping waste is not lower than 30%; the microbial powder is formed by mixing cellulomonas flavigena thalli, an adsorptive carrier and a protective agent in the mass ratio of 1:(0.5-1.5):(0.5-1.5). The adsorptive carrier is selected from dextrin, diatomaceous earth, talcum powder and calcium carbonate; a wetting agent is selected from Tween 80, polyethylene glycol, soluble starch and sodium lauryl sulfate; the protective agent is selected from turf, kaolin and sodium alginate or sodium carboxymethyl cellulose. The microbial powder is used for treating the landscaping waste and can meet the requirement for large-scale composting disposal of the landscaping waste.

Description

technical field [0001] The invention belongs to the technical field of high-efficiency utilization of agricultural and forestry organic resources, and relates to a microbial bacterial agent used for the degradation and disposal of landscaping waste, in particular to an evaluation method for the application of microbial powder for promoting the degradation of landscaping waste. Background technique [0002] In recent years, with the deepening of the concept of a beautiful China and the expansion of the scale of ecological city construction, the level of urban greening and the overall amount of greenery in my country have been continuously improved, and a large amount of landscaping waste has also been generated. Urban garden waste mainly refers to the lawn, litter and tree trimmings generated during the natural or maintenance process of green plants in urban green spaces, street trees and suburban woodlands. These urban garden wastes contain a large amount of starch, cellulos...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10C12N11/14G01G17/00C12R1/01
Inventor 路强强赵宁陈智坤任英英孙晓宇
Owner XIAN BOTANICAL GARDEN SHAANXI PROV
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