A method for removing fluoroquinolone antibiotics in poultry feces
The technology of fluoroquinolones and antibiotics is applied in the field of removing antibiotics in animal manure organic fertilizer. Effect
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Embodiment 1
[0025] The present invention removes the method for fluoroquinolone antibiotics in poultry manure, and its steps are as follows:
[0026] Step 1: Activate the Cellulomonas flavogenes stored on the LB medium, inoculate 2% of the Cellulomonas flavogenes into the selective medium, and shake continuously for 72 hours at a constant temperature of 60°C.
[0027] LB medium: peptone: 10g; yeast extract: 5g; NaCI: 10g; distilled water 1000ml; PH: 7.0.
[0028] Selective medium: KH 2 PO 4 : 0.5g, NaCI: 0.5g, peptone: 0.5g, MgCI 2 : 0.5g, (NH 4 ) SO 4 : 0.5g, NH 4 NO 3 : 1.0g, K 2 HPO 4 : 1.5g, distilled water 1000ml, pH 6.8~7.2.
[0029] Step 2: Mix fresh chicken manure with a moisture content of about 65.0% and an organic carbon content of about 20% with sawdust and distilled water to control the C / N ratio at 20:1 and the moisture content between 55% and 60%. , the amount of bacteria agent is about 5% of the volume of the compost material.
[0030] Step 3: The initial mat...
Embodiment 2
[0038] According to the proportioning of materials and the method of operation of Example 1, the experiment was carried out again. The difference between Example 1 and this Example 2 is: the initial concentration of fluoroquinolone antibiotics in the chicken manure is different. Through three parallel tests, the fluoroquinolone antibiotics The degradation effect is shown in Table 2.
[0039]
[0040] Show from above two examples, through adopting the present invention to process fluoroquinolone antibiotics in chicken manure, make the fluoroquinolone antibiotics of 61.09~82.82% in chicken manure be removed, reduce the harm of chicken manure as organic fertilizer, make wherein fluorine The content of quinolone antibiotics reached the safe limit.
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