Method for removing fluoroquinolones antibiotics in poultry and livestock excrement

A technology of fluoroquinolones and antibiotics, which is applied in the field of removal of antibiotics in poultry and animal manure organic fertilizers, can solve the problems of less research on removal methods, large energy consumption, and large floor area, and achieves easy operation and promotion, and low economic cost Effect

Inactive Publication Date: 2016-01-20
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a small amount of research on the removal of antibiotics in water, such as ultrasonic degradation, artificial wetland, etc. Ultrasonic degradation is fast and has a wide range of applications, but its energy consumption is high, the cost is high, and the reaction conditions are difficult to control; the artificial wetland is low in cost. Easy to maintain but it occupies a large area and emits a foul smell
The research on fluoroquinolone antibiotics in poultry feces mainly stays in its detection and analysis methods, and there are few studies on their removal methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The present invention removes the method for fluoroquinolone antibiotics in poultry manure, and its steps are as follows:

[0026] Step 1: Activate the Cellulomonas flavogenes stored on the LB medium, inoculate 2% of the Cellulomonas flavogenes into the selective medium, and shake continuously for 72 hours at a constant temperature of 60°C.

[0027] LB medium: peptone: 10g; yeast extract: 5g; NaCI: 10g; distilled water 1000ml; PH: 7.0.

[0028] Selective medium: KH 2 PO 4 : 0.5g, NaCI: 0.5g, Peptone: 0.5g, MgCI 2 : 0.5g, (NH 4 ) SO 4 : 0.5g, NH 4 NO 3 : 1.0g, K 2 HPO 4 : 1.5g, distilled water 1000ml, pH 6.8~7.2.

[0029] Step 2: Mix fresh chicken manure with a moisture content of about 65.0% and an organic carbon content of about 20% with sawdust and distilled water to control the C / N ratio at 20:1 and the moisture content between 55% and 60%. , the amount of bacteria agent is about 5% of the volume of the compost material.

[0030] Step 3: The initial materi...

Embodiment 2

[0038] According to the proportioning of materials and the method of operation of Example 1, the experiment was carried out again. The difference between Example 1 and this Example 2 is: the initial concentration of fluoroquinolone antibiotics in the chicken manure is different. Through three parallel tests, the fluoroquinolone antibiotics The degradation effect is shown in Table 2.

[0039]

[0040] Show from above two examples, through adopting the present invention to process fluoroquinolone antibiotics in chicken manure, make the fluoroquinolone antibiotics of 61.09~82.82% in chicken manure be removed, reduce the harm of chicken manure as organic fertilizer, make wherein fluorine The content of quinolone antibiotics reached the safe limit.

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PUM

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Abstract

The invention discloses a method for removing fluoroquinolones antibiotics in poultry and livestock excrement. The method includes the steps of 1, preparing compost raw materials, wherein chicken excrement, sawdust, sterile distilled water and high-temperature-resistant fungicide are prepared into the compost raw materials, the mass ratio of the chicken excrement to the sawdust is 5-10 to 1, the water content of the compost raw materials ranges from 55% to 60%, and the high-temperature-resistant fungicide is cellulomonas flavigena; 2, carrying out high-temperature composting, wherein the pile overturning frequency is controlled to enable the compost temperature to range from 45 DEG C to 65 DEG C, and high-temperature fermentation is achieved; 3, carrying out intermediate-temperature composting, wherein after high-temperature fermentation, the manual pile overturning frequency is controlled to enable the temperature of a compost product to range from 30 DEG C to 45 DEG C, and intermediate-temperature fermentation is achieved. According to the method, the fluoroquinolones antibiotics in the poultry and livestock excrement are removed sufficiently through the degradation effect of high-temperature-resistant strains and relative microorganisms, and therefore the fluoroquinolones antibiotics in organic fertilizers are reduced to the safe level.

Description

technical field [0001] The invention relates to a method for removing antibiotics in organic manure of poultry manure, in particular to a method for removing six fluoroquinolone (FQs) antibiotics. Background technique [0002] Antibiotics are widely used in human medicine, livestock and poultry farming, aquaculture, etc. As animal growth promoters and therapeutic drugs, animal husbandry has become the industry that uses the largest amount of antibiotics. According to statistics, about 50% to 70% of antibiotics consumed each year are used in animal husbandry. After antibiotics enter the animal body, 40% to 90% enter the environment in the form of the mother or metabolites along with the manure of livestock and poultry. As early as 2007, some scholars investigated 143 livestock farms in 8 provinces of my country and found that the survey samples contained high levels of fluoroquinolone antibiotics, among which the concentration of enrofloxacin in pig manure and cow manure was...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F3/00C05F17/00
CPCY02A40/20Y02W30/40
Inventor 薛南冬孟磊张石磊杨兵王继勇
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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