Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Microbial powder and preparation method thereof

A technology of microbial bacteria and bacteria cells, which is applied in the field of efficient utilization of agricultural and forestry organic resources, can solve the problems of unclear key roles and no research on the biological effectiveness of liquid bacteria systems.

Pending Publication Date: 2019-07-05
XIAN BOTANICAL GARDEN SHAANXI PROV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the bacterial strain is still an equal proportion combination of various bacterial liquids. It is not clear which strain plays a key role and how to participate in the degradation process, and the bioavailability of the liquid bacterial strain has not been studied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial powder and preparation method thereof
  • Microbial powder and preparation method thereof
  • Microbial powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This embodiment provides a microbial powder capable of degrading garden cellulose, the effective viable bacteria of which are Cellulomonas flavigena which has a strong ability to degrade cellulose. The Cellulomonas lutea used in this example is a model strain of the genus Cellulomonas, which was purchased from the General Microbiology Center of China Committee for the Collection of Microbial Cultures. In order to prepare a solid microbial powder that is durable and convenient to use, in this example, the liquid fermented cells of Cellulomonas lutea were mixed with a specific ratio of suitable adsorptive carrier, wetting agent and protective agent. Specifically, the adsorptive carrier is selected from one or more of dextrin, diatomaceous earth, talcum powder or calcium carbonate, and in terms of mass ratio, the ratio of the flavin-producing cellulomonas to the adsorptive carrier is 1: (0.5-1.5); Wetting agent selects one or more in Tween 80, polyethylene glycol, soluble ...

Embodiment 2

[0047] This embodiment provides a preparation method of microbial bacterial powder that can degrade garden cellulose, including steps: 1) bacterial liquid fermentation, liquid fermentation of Cellulomonas xanthogenes, and production of Cellulomonas xanthomonas in the fermentation liquid when the fermentation is stopped The number of viable bacteria is not less than 2.0×10 8 pcs / mL; 2) Separation of cells, removal of water in the fermentation liquid to obtain Cellulomonas flavinogenes cells; 3) Preparation of bacteria powder, adding adsorption carrier, wetting agent and protective agent according to the mass ratio, stirring Mix evenly and dry in vacuum to obtain the microbial powder.

[0048] 1) Bacterial liquid fermentation

[0049] The number of effective viable bacteria reaches 2.0×10 8 Cellulomonas flavigena (Cellulomonas flavigena) culture fermentation broth of more than individual / mL is used as seed liquid, inoculates in the fermenter that liquid fermentation is used wi...

Embodiment 3

[0066] This embodiment provides a preferred preparation method of microbial bacterial powder that can degrade garden cellulose, specifically comprising the following steps:

[0067] 1) Bacterial liquid fermentation

[0068] Using the culture medium, prepare 2.5L of fermentation medium, pack in 500mL Erlenmeyer flasks, 200mL per bottle, and sterilize with moist heat (121°C, 103MPa, 30min), inoculate each bottle to produce yellow Cellulomonas bacteria cake (diameter 1.5cm) 2 pieces, constant temperature fermentation on a shaker. The cultivation and fermentation conditions are: temperature 30°C, shaking speed 160rpm, continuous cultivation for 36-48h, until the effective number of viable bacteria in the bacterial liquid reaches 2.0×10 8 Fermentation was stopped when cells / mL was higher.

[0069] 2) Cell isolation

[0070] Under the ambient temperature condition of 25°C, the fermented bacterial liquid was placed in a butterfly separator for 60 minutes, and then subjected to hig...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses microbial powder which comprises cellulomonas flavigena thallus, adsorptive carrier, wetting agent and a protection agent. A mass ratio of the thallus to the adsorptive carrieris 1:(0.5-1.5), a mass ratio of the thallus to the wetting agent is 1:(0.5-1.5), and a mass ratio of the thallus to the protection agent is 1:(0.5-1.5). The adsorptive carrier is selected from dextrin, diatomite, talcum powder or calcium carbonate, the wetting agent is selected from Tween 80, polyethylene glycol, soluble starch or lauryl sodium sulfate, and the protection agent is selected from turf, kaolin, sodium alginate or carboxymethyl cellulose sodium. The microbial powder is prepared through microbial liquid fermentation, thallus separation and microbial powder preparation; in a storage period of 390d, living bacteria count of cellulomonas flavigena is not less than 0.5x108 / g, and activity of cellulase is not lower than 30U / g. The microbial powder can efficiently promote garden cellulose degradation. The preparation process is simple and convenient, and social needs on modular compost treatment of garden greening waste can be met.

Description

technical field [0001] The invention belongs to the technical field of high-efficiency utilization of agricultural and forestry organic resources, and relates to a microbial bacterial agent used for degradation and disposal of garden waste, in particular to a microbial bacterial powder and a preparation method thereof. Background technique [0002] In recent years, with the deepening of the concept of a beautiful China and the expansion of the scale of ecological city construction, the level of urban greening and the overall amount of greenery in my country have been continuously improved, and a large amount of landscaping waste has also been generated. Urban garden waste mainly refers to the lawn, litter and tree trimmings generated during the natural or maintenance process of green plants in urban green spaces, street trees and suburban woodlands. These urban garden wastes contain a large amount of starch, cellulose, lignin and nutrients, which are a reusable organic resou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N1/04C05F17/00C05G3/04C12R1/01C05G3/80
CPCC12N1/20C12N1/04C05F11/00C05F17/00C05G3/80C05F11/02C05F11/08Y02W30/40
Inventor 路强强赵叶子陈智坤任英英孙晓宇
Owner XIAN BOTANICAL GARDEN SHAANXI PROV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com