Culture medium for morchella esculenta cultivated species as well as preparation method and application of culture medium
A technique for cultivation of Morchella, culture medium, applied in directions such as botanical equipment and methods, application, cultivation, etc., can solve problems such as yield impact, and achieve the effects of low cost, thick mycelium, and fast microbial growth
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Embodiment 1
[0028] (1) Morchella cultivar medium formula
[0029] Substrate: 50 parts of sorghum grains, 35 parts of corn stalks, 13 parts of enoki mushroom chaff, 2 parts of gypsum powder.
[0030] Composite bacterial species: by weight ratio, Cellulomonas flavinogenes: Actinomyces roseosa=1:1, and the addition amount is 1% of the matrix weight.
[0031] Water: add water to adjust the water content to 63%.
[0032] (2) preparation method of hickory chick cultivar substratum
[0033] 1) Take sorghum grains, corn stalks, enoki mushroom chaff and gypsum powder according to the above parts by weight;
[0034] 2) Pre-treatment of sorghum grains: the sorghum grains are required to be dry and free from mildew, and the sorghum grains are pre-boiled in water until half-cooked, then removed and dried;
[0035] 3) Pre-treatment of corn stalks: the stalks are required to be dry without mildew, crush the stalks to a particle size of less than 1 cm, add water to moisten them, first adjust the conte...
Embodiment 2
[0045] (1) Morchella cultivar medium formula
[0046]Substrate: 40 parts of sorghum grains, 43 parts of corn stalks, 15 parts of enoki mushroom chaff, 2 parts of gypsum powder.
[0047] Composite bacterial species: by weight ratio, Cellulomonas flavinogenes: Actinomyces roseosa=1:1, and the addition amount is 1% of the matrix weight.
[0048] Water: add water to adjust the water content to 63%.
[0049] (2) preparation method of hickory chick cultivar medium: same as embodiment 1
[0050] (3) Application
[0051] 1) Steam sterilize the hickory chick cultivar culture medium, keep the temperature at 90°C-100°C for 2h, then lower the temperature to 55°C-60°C and keep it for 40-60min; cool to room temperature.
[0052] 2) Put the sterilized culture medium into a polyethylene plastic bag of 17cm×33cm×0.04cm. The culture medium should be tightly packed, and a hole should be punched in the middle with a hole diameter of 1.5cm;
[0053] 4) Insert 8ml of Morchella liquid strain und...
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