Method for extracting tea saponin from tea seed meal with microbial fermentation method

Abstract

The invention relates to a method for extracting tea saponin from tea seed meal with a microbial fermentation method. The method is characterized by comprising steps as follows: taking crushed dry tea seed meal as a raw material, taking water as a solvent, adding a proper amount of growth factors (NH4)2SO4, KH2PO4 and K2HPO4, taking Aspergillus niger, Cellulomonas flavigena, saccharomycetes and bacillus subtilis which are sequentially added as fermentation media, preparing a tea saponin coarse extract through multiple times of fermentation treatment, and finally, purifying the extract with a macroporous resin method to obtain the tea saponin with the purity higher than 85%.

Description

technical field [0001] The invention relates to the deep processing technology of tea seed meal, in particular to a method for extracting tea saponin from tea seed meal by microbial fermentation. Background technique [0002] my country is a big tea-producing country. The planting area of ​​camellia oleifera and the output of camellia oleifera seeds account for about 95% of the world. When the picked camellia oleifera fruit is processed into oil, a large amount of by-products of camellia seed meal will be produced. The tea saponin in tea seed meal is a non-ionic natural surfactant with excellent performance. Its content varies from 4% to 14% in different tea seed meal. It can be widely used in light industry, chemical industry, Pesticides, feed, breeding, textiles, oil extraction, mining, and building materials. Tea saponin is a triterpenoid saponin, which is composed of sugar body, aglycone and organic acid. One end of the sugar body is a hydrophilic group, which is conne...

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Problems solved by technology

[0005] According to the inventor's investigation on the process of obtaining tea saponin from tea seed dregs, the disadvantages of using the organic solvent method to extract poor saponin are that the solvent consumption is large, the production cost is high, and it cannot be used for large-scale production. With the continuous improvement of environmental protection awareness, this method of solvent extraction not only has the recovery and treatment of solvents in the later stage, but also has the problem of solvent pollution to the environment, so it should be gradually withdrawn.

[0006] Using biological enzymatic hydrolysis to extract tea soap from shelled camellia oleifera seed powder is quite advanced in terms of the method itself, but due to the high price of biological enzymes, it is unavoidable to use the cost of extracting tea soap from tea seed powder too high problem

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