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Preparation and use method of thermophilic microbial agent for drying leather sludge

A technology of thermophilic microorganisms and sludge drying, which is applied in the field of preparation of thermophilic microbial bacterial agents, can solve problems such as environmental safety hazards and social impacts, and achieve good effects, eliminate odors, and reduce water content.

Inactive Publication Date: 2019-12-20
湖南洁源生物能源科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional disposal methods (landfill, composting, digestion and incineration and other technologies) all have environmental safety hazards and social impacts

Method used

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  • Preparation and use method of thermophilic microbial agent for drying leather sludge

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preparation example Construction

[0021] A preparation method for a thermophilic microbial bacterial agent for drying leather sludge, the preparation method comprising the following steps:

[0022] Streptococcus thermophilus ( Streptococcus thermophilus ), Bacillus coagulans ( Bacillus coagulans ), Bacillus subtilis ( Bacillus subtilis subsp. subtilis ), Cellulomonas flavogenes ( Cellulomonas flavigena ), Candida utilis ( Candida utilis ), Rhizopus oryzae ( Rhizopus oryzae ) and Trichoderma viride ( Trichoderma viride ) strains were respectively activated and liquid expanded culture to culture medium OD 600 It is 1.0-2.8, and the culture liquid of each bacterial strain is obtained;

[0023] Wherein, the cultivation steps of Streptococcus thermophilus, Bacillus coagulans, Bacillus subtilis and Cellulomonas xanthogenetica strains are as follows: Afterwards, inoculate in liquid medium for expansion culture, shake rate 100-400 rpm, temperature 35-38ºC, pH 6.5-8.0, culture to logarithmic phase of ...

Embodiment 1

[0034] A thermophilic microbial agent for efficient biological drying of leather sludge, the thermophilic microbial agent is composed of Streptococcus thermophilus, Bacillus coagulans, Bacillus subtilis, Cellulomonas chrysogenum, Candida utilis Yeast, Rhizopus oryzae and Trichoderma viride are mixed and formulated. Among them, Streptococcus thermophilus, Bacillus coagulans, Bacillus subtilis and Cellulomonas xanthogenes were activated and cultured on the inclined plane of TSB medium, under the condition of shaking rate 200 rpm, 36ºC, pH 7.0, the liquid expanded culture until the culture Liquid OD 600 was 2.5, and then respectively obtained the bacterium liquid of Streptococcus thermophilus, Bacillus coagulans, Bacillus subtilis, and Cellulomonas lutea; , under the condition of pH 6.5, the liquid expansion culture was cultivated until the culture medium OD 600 2.6, to obtain the bacterium liquid of Candida utilis; Rhizopus oryzae and Trichoderma viride were activated and cult...

Embodiment 2

[0039] A thermophilic microbial agent for efficient biological drying of leather sludge, the thermophilic microbial agent is composed of Streptococcus thermophilus, Bacillus coagulans, Bacillus subtilis, Cellulomonas chrysogenum, Candida utilis Yeast, Rhizopus oryzae and Trichoderma viride are mixed and formulated. Among them, Streptococcus thermophilus, Bacillus coagulans, Bacillus subtilis and Cellulomonas xanthogenes were activated and cultured on the inclined plane of TSB medium, under the condition of shaking rate of 180 rpm, 38ºC, pH 7.5, the liquid expanded culture until the culture Liquid OD 600 was 2.8, and then respectively obtained the bacterial liquids of Streptococcus thermophilus, Bacillus coagulans, Bacillus subtilis, and Cellulomonas lutea; after Candida utilis was activated and cultured on the slant of LB medium, it was shaken at a shaking rate of 190 rpm at 27ºC. , under the condition of pH 6.0, the liquid expansion culture was cultivated until the culture m...

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Abstract

The invention relates to the technical field of fermentation biology, in particular to a preparation and use method of a thermophilic microbial agent for drying leather sludge. According to the invention, scientific and reasonable mixing and compounding are carried out according to the functions of thermophilic microorganisms such as streptococcus thermophilus, bacillus coagulans, lactobacillus acidophilus, cellulomonas flavigena, candida utilis, rhizopus oryzae and trichoderma viride, so that the thermophilic microorganism mixed flora is in an optimal growth state in the biological drying process of leather sludge, and the strains are mutually coordinated and not mutually antagonized. The microbial agent effectively inhibits the growth of harmful microorganisms, greatly improves the degradation efficiency and the deodorization effect of the leather sludge, greatly shortens the sludge drying time, remarkably reduces the water content of the leather sludge, has good sludge decrement effect, and has the advantages of high removal rate of sulfur and ammonia nitrogen, simple drying process, no toxicity and corrosion, convenient operation, small production investment, low production cost, no secondary pollution and stable sludge decrement.

Description

technical field [0001] The invention relates to the technical field of fermentation biology, in particular to a method for preparing and using a thermophilic microbial agent for drying leather sludge. Background technique [0002] While obtaining good economic and social benefits, the leather industry also produces a large amount of leather sewage. Every 10,000 tons of leather sewage can produce 10-20 tons of dry-based sludge. The sludge contains bacterial micelles formed by various microorganisms, organic debris, Inorganic particles, colloids and other extremely complex heterogeneous bodies contain salts and a small amount of pathogenic microorganisms and parasite eggs. The water content can be as high as 90% or more, and the content of refractory and corrupt organic matter is high, giving off a pungent stench. Therefore, leather sludge must be dried before it is discharged and utilized to reduce the water content of the sludge. Traditional disposal methods (landfill, comp...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14C02F11/12C02F11/02C12R1/46C12R1/07C12R1/125C12R1/72C12R1/845C12R1/885C12R1/01
CPCC12N1/20C12N1/16C12N1/14C02F11/12C02F11/02
Inventor 曾虹燕张栋张卫平翁凯敏徐圣
Owner 湖南洁源生物能源科技有限公司
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