The invention provides a preparation method of an immortalized
duck embryo hepatic
cell line. The preparation method comprises following steps: (1)
duck embryo liver is subjected to
digestion with trypsase, and an obtained digestive juice is subjected to culturing so as to obtain primary cultured
duck embryo hepatic cells; (2) lipidosome
transfection is adopted, and eukaryotic expression plasmids used for coding hTERT and Marker genes are introduced into the primary cultured duck
embryo hepatic cells; (3) obtained transfected duck
embryo hepatic cells are subjected to culturing, and hTERT positive clones are selected with G418; and (4) selected hTERT positive clones are subjected to culturing, and the immortalized duck
embryo hepatic
cell line is obtained after more than 50 generations of continuous culturing. The immortalized duck embryo hepatic
cell line is capable of maintaining relatively excellent activity after more than 50 generations of
in vitro continuous
cell culture; division and proliferation can be maintained; no aging or
apoptosis is caused; and immortalization of the immortalized duck embryo hepatic cell line is realized. The immortalized duck embryo hepatic cell line is regular in
cell morphology; culturing conditions are simple and are easy to control; using is convenient; and an excellent carrier is provided for research on duck
disease, especially on
duck hepatitis virus.