54 results about "Alpha amylase inhibitor" patented technology

The invention discloses a method for industrially extracting an alpha-amylase inhibitor in white kidney beans, belonging to the field of preparation of plant effective components. The method comprises the following steps: firstly adding water into soaked and peeled white kidney beans, grinding into slurry, sieving to remove residues, performing standing, centrifuging, enzyme deactivation, centrifuging and ethanol precipitation on an obtained filtrate to obtain protein, dissolving the obtained protein by using a phosphate buffer solution, performing moderate enzyme hydrolysis by using alkaline protease to change isoelectric points of impurity protein, then performing acid precipitation, collecting supernatant liquid, and performing ethanol precipitation and freeze drying to obtain an alpha-amylase inhibitor product. The prepared alpha-amylase inhibitor disclosed by the invention is high in purity and strong in activity; and moreover, the alpha-amylase inhibitor needs simple equipment, is low in cost, is pollution-free, has relatively good economic and environmental benefits, and can be applied to industrial large-scale production.

The present invention provides a plant-derived carbohydrase inhibitor, wherein the inhibitor is effective for preventing or alleviating diabetes, or preventing obesity, and foods, drinks, and medicines containing the same. The present invention is accomplished by use of an alpha-amylase inhibitor or an alpha-glucosidase inhibitor as a carbohydrase inhibitor that is extracted from astringent skins of a chestnut using ethanol or aqueous ethanol solution. The present invention can also be accomplished by adding the carbohydrase inhibitor to a food or medical composition as an active ingredient for delaying saccharide digestion or absorption, suppressing a rise in postprandial blood glucose levels or blood insulin levels, or preventing obesity.

The invention discloses a method for preparing an alpha-amylase inhibitor by an enzymatic method. In the method, white kidney beans are used as raw materials and are subjected to soaking, grinding and centrifugating; supernatant fluid is processed by steps by complex enzyme and is subjected to ion-exchange chromatography and hyperfiltration to prepare the alpha-amylase inhibitor. In the method, the white kidney beans are used as raw materials and the alpha-amylase inhibitor is prepared by the enzymatic method, therefore, the alpha-amylase inhibitor contained in the white kidney beans is effectively released to conduce to extraction and purification, thus reducing extraction cost. The method has mild reaction conditions, high purity of obtained products, good effect on inhibiting the amylase and inhibition activity as high as 70-95%, and can be widely applied in such fields as medicines, health-care food and the like.

This invention relates to the combination of α-amylase inhibitors prepared from Phaseolus vulgaris with α-glucosidase inhibitors obtained from Salacia oblonga and other species. The α-amylase inhibitor is accompanied by a quantity of lectins that reduces the amount of glucose originating from the starches present in the diet, and considerably reduces the appetite after repeated administration. The combination with α-glucosidase inhibitors, such as extracts of Salacia or the thiosugars present in it, further reduce the blood glucose, acting synergically, and consequently reduce the synthesis of fats from carbohydrates.

A nutritional supplement composition contains inhibitors of alpha-glucosidase and alpha-amylase substantially in the absence of lipase inhibitors. The composition can include touchi extract and phaseolamin as the alpha-glucosidase and alpha-amylase inhibitor, respectively. A method of limiting the absorption of carbohydrates contained in a foodstuff includes administering the nutritional supplement composition prior to consumption of the carbohydrates, and a method of promoting weight loss in an individual includes administering the nutritional supplement composition to the individual over a period of days.

The invention provides a method for preparing deproteinized rice and a product and for jointly preparing an alpha-amylase inhibitor and a protein peptide. The preparation method specifically comprisesthe following steps: washed rice or rice flour is used as a raw material; after sterilization, first enzymolysis is carried out by using an enzymatic hydrolysate to prepare alpha-amylase inhibitory peptide; after a solid material obtained by the first enzymolysis is sterilized, second enzymolysis is conducted and a second enzymatic hydrolysate is used to prepare protein peptide; a solid materialobtained by the second enzymolysis is directly steamed, dried and dispersed to obtain deproteinized rice or rice flour containing resistant starch, or is used to prepare box lunch; specific protease is added during the first enzymolysis, or zymophyte and specific protease are simultaneously added to perform enzymolysis and fermentation; and non-specific protease and triglyceride lipase are added during the second enzymolysis. According to the invention, contents of protein, sodium, potassium and phosphorus in rice are reduced, the alpha-amylase inhibitor and the antioxidant and ACEI-activity protein peptide are jointly prepared, and the content of resistant starch in deproteinized rice is increased.

The invention belongs to the technical field of medicine. The invention relates to application of tetrahydro isoquinoline alkaloid which is shown in a formula (I) or a formula (II) or a pharmaceutically acceptable salt of the tetrahydro isoquinoline alkaloid in an aspect of preparing alpha-amylase inhibitors. Activity screening experiments prove that the compound disclosed by the invention has an effect of inhibiting activity of two kinds of in-vitro digestive enzyme of alpha-amylase and pancreatic lipase and inhibiting proliferation and differentiation activity of 3T3-L1 preadipocyte at the same time and has an application prospect in an aspect of clinical research on diabetes, hyperlipemia, obesity or metabolic syndrome.

The invention discloses a method for screening an alpha-amylase inhibitor. The method comprises: preparing an enzymatic reaction buffer solution, a substrate solution, an alpha-amylase solution, a color-developing reagent solution, and a to-be-tested product solution for later use; performing sample application of the to-be-tested product solution on a thin layer plate; allowing a solvent to be volatilized or using a proper developing solvent for developing, performing air-drying, and soaking the obtained product in the substrate solution; taking the obtained product out, allowing the solvent to be volatilized, soaking the obtained product in the alpha-amylase solution, and carrying out an enzymatic reaction at 5-50 DEG C for 10-60 min; after the reaction is completed, taking the obtained thin layer plate out, allowing the solvent to be volatilized, soaking the obtained product in the color-developing reagent solution for a color-developing reaction; and viewing the obtained thin layer plate in visible light. Blue spots which appear in the white or yellow or brown background are the alpha-amylase inhibitor. The invention provides the method which enables the alpha-amylase inhibitor to be conveniently fast screened and provides an ideal approach for screening active ingredients containing the alpha-amylase inhibitor from natural substances.

The invention discloses the application of an alpha-amylase inhibitor in preparing a medicine for preventing and curing hyperlipidemia and atherosclerosis, where the medicine can prevent and cure hyperlipidemia and atherosclerosis, safe, nontoxic, having good endurance and especially suitable to be applied long term.