30 results about "Acinetobacter johnsonii" patented technology

The invention provides a method for removing mechanical impurities in the dirty oil with microorganism, solving the problem of undesirable effect for removing the mechanical impurities in the dirty oil in the existing method for purifying the dirty oil. The method comprises the steps of: (1) inoculating acinetobacter johnsonii into a liquid LB culture medium to carry out accumulation culture, and controlling the cell concentration of culture solution OD=1.1-1.2; and (2) mixing the dirty oil with water according a certain volume ratio, heating up oil water mixture at 37 DEG C and keeping temperature, adding the culture solution obtained by the step (1) into the oil water mixture according to the inoculating quantity, the volume of which is 2-10% of that of the oil water mixture, simultaneously aerating, heating up at 60 DEG C to 90 DEG C after aerating and keeping temperature for 6-8 hours, and stopping heating up. The method can effectively reduce the content of the mechanical impurities in the dirty oil, thereby preventing the mechanical impurities from forming into deposition in a pipe line or equipment and being capable of guaranteeing the running stability of a joint station.

The invention discloses a fully inactivated vaccine for preventing and curing giant spiny frog canker. A preparation method of the fully inactivated vaccine comprises the following steps: (1) taking acinetobacter junii, acinetobacter lwoffii and acinetobacter johnsonii as pathogenic bacteria, and treating each pathogenic bacterium in such a way that a formaldehyde solution is added into a pathogenic bacterium suspension with the OD600 value of 0.2 to prepare a vaccine with the formaldehyde concentration of 0.2% and the vaccine is inactivated in an incubator at 37 DEG C for 96 h, thus obtaining the formaldehyde inactivated vaccines of the three pathogenic bacteria respectively; (2) washing and then centrifuging the formaldehyde inactivated vaccines of the three pathogenic bacteria so as to obtain the treated inactivated vaccines of the three pathogenic bacteria respectively; and (3) mixing the treated inactivated vaccines of the three pathogenic bacteria at the volume ratio of 1:1:1, and then concentrating the mixture to 1 / 5 of the original total volume to obtain the fully inactivated vaccine for preventing and curing giant spiny frog canker. The fully inactivated vaccine provided by the invention can be used for preventing and curing giant spiny frog canker and has high popularization and application values.

The invention relates to acinetobacter johnsonii LP28 and a method for preparing low-temperature alkali lipase by using the acinetobacter johnsonii. The strain is preserved in CGMCC, the preservation number is CGMCC 3069, and the homology of a 16S rDNA sequence of the strain and DQ257426 is as high as 100 percent. The method for preparing the low-temperature alkali lipase comprises the following steps: (1) screening bacterial strains; (2) seed cultivation; (3) fermentation and cultivation; and (4) separation and purification. The acinetobacter johnsonii LP28 is from the soil polluted by oil in a saline and alkaline land of Tianjin Bohai Bay, the enzyme production is stable, the raw material source is wide, and the production cost is low; and the obtained low-temperature alkali lipase has good stability for additive components of a washing agent such as surfactant, oxidant, protease and the like, and can be used as enzyme added into the washing agent to be applied in the industry of the washing agent.