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Microbial agent suitable for corn planting, and application thereof

A technology of microbial inoculum and corn, applied in the field of microorganisms, can solve the problems of PGPR production stagnation and non-industrial application, etc.

Active Publication Date: 2021-07-30
GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(Salas-González I, Reyt G, Flis P, et al. Coordination between microbiota and root endodermis supports plant mineral nutrient homeostasis [J]. Science, 2021, 371 (6525)), although the research institutions have made great achievements in the research of PGPR Fruitful results, but at present, the production of PGPR is still in the laboratory and has not been applied industrially. There is no relevant report on the effect of PGPR on plant growth promotion in experimental fields.

Method used

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  • Microbial agent suitable for corn planting, and application thereof
  • Microbial agent suitable for corn planting, and application thereof
  • Microbial agent suitable for corn planting, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1, activation and rejuvenation single strain and the seed liquid of cultivating microbial inoculum

[0073] The purchased Acinetobacter johnsonii, Acinetobacter lwoffii, Brevundimonas nasdae, Burkholderia cepacia, para Microbacterium paraoxydans, Pseudomonas aeruginosa, Pseudomonas guguanensis, Pseudomonas moraviensis, Pseudomonas nitroreduction ( Pseudomonas nitroreducens), Pseudomonas taiwanensis, Rhodococcus ruber, Serratia marcescens, Novosphingobium resinovorum, a total of 13 strains The lyophilized material was recovered for culture.

[0074] The specific operation is: use a sterile pipette to draw 0.3mL of the corresponding culture medium and drop it into the ampoule bottle, shake it to dissolve the freeze-dried product; draw up all the bacterial liquid in the ampoule tube, inoculate it in the corresponding culture medium test tube and let it stand for culture. Activation of individual strains was completed one day later.

[0075] Add the activate...

Embodiment 2

[0076] Embodiment 2, co-culture microbial bacterial agent

[0077]Microbial agents include Microbiota A, Microbiota B and Microbiota C, Microbiota A is composed of Acinetobacter ruckeri and Pseudomonas Guguana, and Microbiota B is composed of Acinetobacter johnsonii and Pseudomonas nitroreduction Microbiome C was composed of Microbacterium paraoxidans and Pseudomonas guguana.

[0078] In this embodiment, microbiome A is composed of 10% Acinetobacter ruckeri and 90% Pseudomonas guiseki; microbiome B is 10% Acinetobacter yoshii Bacillus and Pseudomonas nitroreductors with a quantity ratio of 90%; Microbiome C consisted of Microbacterium paraoxidans with a quantity ratio of 10% and Pseudomonas guguan with a quantity ratio of 90%.

[0079] Microbiome A, microbiome B, and microbiome C were added to the corresponding co-culture medium, placed in a constant temperature shaking shaker for cultivation, and the parameters of the shaker were set to 28°C, 150r / min, and after 24-48h, th...

Embodiment 3

[0085] Embodiment 3, the experiment that corn absorbs beneficial mineral elements in soil under the influence of single strain

[0086] 1) For the detoxified Zhengdan 958 corn seeds, the experimental group soaked the seeds with 13 kinds of single-plant strains diluted in Example 1, and the control group soaked the seeds with the culture solution of the corresponding dilution multiple, totally 14 groups. Dilution standard for seed soaking: evaluate the cell concentration of the cell solution according to the OD value of the cell solution and the growth curve, so that the cell concentration after dilution is 10 4 ~10 7 CFU / mL. After soaking the seeds for 30 minutes, sow them in a cultivation pot filled with humus soil, the sowing depth is 3-5cm. Before sowing, ensure that the soil can be agglomerated by hand, and discard the pieces; after sowing, put them in a constant temperature incubator for cultivation. The parameters are 16 hours of light and 8 hours of darkness; illumi...

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Abstract

The invention relates to the technical field of microorganisms, and particularly discloses a microbial agent suitable for corn planting, and application thereof. The microbial agent disclosed by the invention comprises at least one of the following strains: acinetobacter johnsonii, acinetobacter lwoffii, brevundimonas natans, burkholderia cepacia, microbacterium paraoxidans, pseudomonas aeruginosa, pseudomonas oryzae, pseudomonas ferrawiae, pseudomonas nitroreducens, pseudomonas formosana, rhodococcus ruber. serratia marcescens and neosphingosine resinophila. The microbial agent provided by the invention can promote absorption of beneficial mineral elements by corn, improves the problem of enrichment of heavy metals in soil by corn in an existing planting system, and reduces the heavy metal content of corn; moreover, compared with a conventional corn planting method, a planting method in the invention has the advantages that the corn germination speed and germination rate can be better increased, and the development condition of the root system of the corn in the seedling stage and the leaf surface development condition of the corn in the seedling stage can be improved.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a microbial agent suitable for planting corn and its application. Background technique [0002] Corn is an annual herbaceous plant of Poaceae. At the beginning of the 21st century in my country, the perennial corn planting area was as high as 2.453*107hm 2 , the total output value is as high as 1.2317*108t. Corn has become an important raw material for food, feed, fermentation industry and thousands of fine chemical products, and plays a pivotal role in global food safety and national economic development. Under the existing planting system, it is basically completed in the order of soaking seeds, planting, base fertilizer, seedling lifting, top dressing, foliar fertilization, weeding, and harvesting. [0003] In 1978, American botanists first proposed the concept of plant growth-promoting rhizobacter (PGPR). When inoculated in plant seeds, roots, tubers, tubers or soil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01C1/06A01G7/06A01G22/20A01N63/20A01N63/27A01P21/00C12R1/01C12R1/385C12R1/38C12R1/425
CPCC12N1/20A01C1/06A01G7/06A01G22/20A01N63/20A01N63/27
Inventor 沈家葆李华一
Owner GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
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