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Acinetobacter johnsonii AJ-3 strain and application thereof

A technology of AJ-3 and bacteria strains, applied in the field of environmental microorganisms, can solve the problems of high treatment costs, large dosage, large amount of chemical sludge, etc., and achieve strong environmental adaptability, high phosphorus removal efficiency, and low requirements for dissolved oxygen Effect

Active Publication Date: 2014-05-21
威海海能海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical phosphorus removal is mainly a chemical precipitation method, which has the advantages of simple operation, high removal rate, and stable operation; The final disposition of the

Method used

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  • Acinetobacter johnsonii AJ-3 strain and application thereof
  • Acinetobacter johnsonii AJ-3 strain and application thereof
  • Acinetobacter johnsonii AJ-3 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Isolation, screening and identification of high-efficiency phosphorus-accumulating bacterium Acinetobacter johnsonii AJ-3 strain

[0023] (1) Medium

[0024] Beef extract peptone solid medium: beef extract 3 g / L, peptone 10 g / L, NaCl 5 g / L, agar 20 g / L, pH 7.0-7.2 (liquid medium without agar);

[0025] Phosphorus-limited (phosphorus-rich) solid medium: Dissolve 8.372 g of 3-morpholine propanesulfonic acid and 0.717 g of N-tris(hydroxymethyl)methylglycine in 30 mL of deionized water, and adjust the pH with 10 mol / L KOH To 7.4, add solutions in the following order: 0.01 mL 1.830% FeSO 4 solution (prepared), 5 mL 1.9 mol / L NH 4 Cl, 1 mL 0.276 mol / L K 2 SO 4 , 0.025 mL 0.02 mol / L CaCl 2 2H 2 O, 0.42 mL of 1.25 mol / L MgCl 2 6H 2 O, 20 mL 2.5 mol / L NaCl, 0.02 mL trace element mixed solution (ammonium heptamolybdate 3×10 -6 mol / L, H 3 BO 3 4×10 -4 mol / L, CoCl 2 3×10 -5 mol / L, CuSO 4 10 -5 mol / L, MnCl 2 8×10 -5 mol / L, ZnSO 4 10 -5 mol / L)...

Embodiment 2

[0037] The growth curve determination of embodiment 2 Johnson's Acinetobacter AJ-3 bacterial strain

[0038] (1) Strain: AJ-3 strain.

[0039] (2) Medium

[0040] Beef extract peptone solid medium: the formula is the same as in Example 1

[0041] Synthetic sewage liquid medium: sodium acetate 0.925 g / L, peptone 0.1 g / L, yeast extract 0.01 g / L, NaCl 0.05 g / L, KH 2 PO 4 0.0655 g / L, MgCl 2 ·6H 2 O 0.1412 g / L, CaCl 2 0.025 g / L, pH7.0-7.2.

[0042] (3) Method

[0043] Take 20 μL of glycerol bacteria stored at -20°C and spread it on the plate of beef extract peptone solid medium, culture it at 28°C for 24 hours, pick a single colony and mix it with 0.3 mL sterile water, take 100 μL and spread it on beef extract On the plate of peptone solid medium, use a hole puncher (pore diameter 0.5 cm) to take 2 holes and connect it to 15 mL beef extract peptone liquid medium, and culture it in a shaker at 28 °C and 170 r / min for 24 h (OD 600 ≈0.9) was activated as seed solution; the...

Embodiment 3

[0046] Example 3 The effect of liquid volume on the biological phosphorus removal effect of Acinetobacter johnsonii strain AJ-3.

[0047] (1) Strain: AJ-3 strain.

[0048] (2) Medium

[0049] Beef extract peptone solid medium: the formula is the same as in Example 1;

[0050] Synthetic sewage liquid culture medium: formula is the same as embodiment 2.

[0051] (3) Method

[0052] Take 20 μL of glycerol bacteria stored at -20°C and spread it on the plate of beef extract peptone solid medium, culture it at 28°C for 24 hours, pick a single colony and mix it with 0.3 mL sterile water, take 100 μL and spread it on beef extract On the plate of peptone solid medium, use a hole puncher (pore diameter 0.5 cm) to take 2 holes and connect it to 15 mL beef extract peptone liquid medium, and culture it in a shaker at 28 °C and 170 r / min for 24 h (OD 600 ≈0.9) to be activated as seed solution; then 8% of the inoculum volume was inserted into liquid medium containing different amounts of...

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Abstract

The invention belongs to the field of environmental microorganisms and in particular relates to separation and culture of an Acinetobacter johnsonii AJ-3 strain and application thereof in phosphorus removal in sewage and biological phosphorus removal in urban sewage treatment plants. The Acinetobacter johnsonii AJ-3 strain has a collection number of CCTCCM2014023, the collection place is China Center for Type Culture Collection, and the collection date is January, 16th 2014. The strain is an Acinetobacter johnsonii AJ-3 strain, is rapid in growth, high in adaptive capacity to environment and low in dissolved oxygen demands and can rapidly grow in a phosphorus-containing synthetic sewage liquid culture medium, the phosphorus is removed, the phosphorus removal efficiency is high and stable and reaches 55-60.1%, and secondary pollution is avoided. Therefore, the AJ-3 strain can be applied to phosphorus-containing sewage treatment, is an important microbial resource in a water organism phosphorus removal process and has wide application prospect.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, in particular to a strain of Acinetobacter johnsonii ( Acinetobacter johnsonii ) The isolation and cultivation of AJ-3 strain and its application in sewage phosphorus removal, which is applied to the biological phosphorus removal of urban sewage treatment plants. Background technique [0002] From 2010 to 2012, the Bulletin on the State of the Environment in China showed that the eutrophication of 62 key lakes (reservoirs) controlled by the state was prominent, and the water quality in the coastal waters of the East China Sea was extremely poor and heavily polluted in the past three years, and the main pollution indicators included Phosphorus; and eutrophication will reduce the transparency of water bodies, produce harmful gases and biotoxins, affect the health of humans and animals, destroy biodiversity in water bodies, affect tourism and shipping, etc.; therefore, removing phosphorus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/10
Inventor 蒋冬花谢祥聪胡优李晓倩
Owner 威海海能海洋生物科技有限公司
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