45results about How to "Short marking time" patented technology

The invention provides an RGD polypeptide coordination complex of a radioactive nuclide label. According to the structure of the coordination complex, the radioactive nuclide is selected from 64Cu, 68Ga, 111In, 62Cu, 67Cu, 67Ga, 86Y, 89Zr or 18F; the ligand is a ligand compound containing an RGD structure, which is shown in a formula (I). The coordination complex has stronger ligand stability and high target / non-target ratio, and the appetency of the ligand with integrin AlphavBeta3 is stronger. The invention further provides a preparation method of the coordination complex, and an application of the coordination complex in preparing tumor developers.

The invention discloses an in-vitro mark for fish and a mark gun of the in-vitro mark. The mark comprises a U-shaped body made of plastic material; a pin is arranged at the inner side of one end of the U-shaped body, and a pin hole corresponding to the pin is arranged at the other end. The mark gun utilizes the mark; the pin on the mark can pass through fin rays on the fish body and is fixed in the pin hole on the U-shaped body to form an annular mark, which is firmly fixed on the base part of each fin ray just as a ring, and an opening of membrane between the fin rays is not increased so as to solve the problem that the mark easily falls off. Only the fin rays are marked; muscle of the fish body is not damaged; the fish death can be reduced, and the survival rate of the discharged fish can be ensured. Meanwhile, the quantity of the marks is large, so that a method for marking by the mark gun is fast; the marking time can be reduced, and the work efficiency can be improved.

The invention discloses a scribing device which comprises a base, a first support frame and a second support frame, wherein the first support frame and the second support frame are fixedly arranged on the upper end face of the base; support tables for supporting scribed workpieces are respectively arranged at the upper ends of the first support frame and the second support frame; a sliding block which can slide on the upper end face of the base is arranged on the upper end face of the base; an upright post is fixedly arranged on the sliding block; a scribing fixed rod is fixedly arranged at the upper end of the upright post; a scribing needle which is fixedly arranged at one end of the scribing fixed rod is arranged at the upper end of the upright post; and a staff gauge is arranged on the base at one side of the sliding block. Workpieces are placed on the support tables at the upper ends of the first support frame and the second support frame, so that one end of each workpiece is aligned to a needle head of the scribing needle; the sliding block is moved within a stroke of a guide rail according to the required scribing length; the staff gauge can be used for accurately measuring the stroke of the scribing block; after accurate positioning, the scribing needle is pressed to scribe on the workpieces and further the workpieces can be accurately scribed. Therefore, the scribing device disclosed by the invention has the advantages of ensured scribing precision, short scribing time and high efficiency.

The invention discloses a targeted probe for nuclide labeling and a preparation method and an application of the targeted probe. A general structural formula of a complex is as shown in the specification, wherein R' is a nuclide chelating group; R is a targeted group; Dn is a molecular skeleton which is formed by repeated michael addition reaction and amidation reaction employing propargylamine as an initial reactant, the peripheral group is amino, n represents different algebras, and the numerical value of n is an integer greater than or equal to 0; and m is equal to 2n. A polymer in the targeted probe for nuclide labeling is beneficial to improvement of the specific activity; a high-quality developing result can be obtained by instrument scanning; the targeted probe can play a role in effectively monitoring tumors or inflammatory diseases; meanwhile, carrying of relatively many nuclides on a molecule is facilitated by the formed polymer; the nuclide concentration of focus location is improved; and the targeted probe plays a relatively good treatment role.

The invention discloses a preparation method of a gold immunochromatography assay test strip indirectly connecting colloidal gold using a goat-anti-mouse secondary antibody with an anti-mouse marker.The preparation method comprises the following steps: (1) preparing a colloidal gold solution by using trisodium citrate to reduce chloroauric acid; (2) using the colloidal gold solution to mark the goat-anti-mouse secondary antibody; (3) adding the to-be-marked mouse antibody into the colloidal gold solution marking the goat-anti-mouse secondary antibody; (4) processing the indirectly marked mouse antibody colloidal gold solution onto a conjugate pad; (5) processing a corresponding antigen onto a nitric acid cellulose membrane; (6) adhering the nitric acid cellulose membrane, water absorptionpaper, the conjugate pad and a sample pad to a PVC plastic bottom plate; and (7) identifying the quality of the prepared gold immunochromatogrpahy assay test strip according to the product quality standard. The preparation method has the advantages that the detection sensitivity of the gold immunochromatogrpahy assay test strip can be improved, the consumption of antibodies used by the marker canbe reduced, the cost is reduced, the performance of the test paper is improved, and the detection result is stable and reliable.

The invention relates to a <18>O on-line marked protein quantitative analysis platform, and an operation method thereof. The <18>O on-line marked protein quantitative analysis platform is a system integrating protein on-line enzymolysis, <18>O on-line marking and polypeptide separation and detection. The <18>O on-line marked protein quantitative analysis platform comprises an injection pump, a sampling needle, a column oven, an enzyme reactor, a switch valve, a peptide fragment capture column, a liquid phase chromatography pump, a polypeptide separation column and a mass spectrometry detector. A protein sample is firstly loaded to the enzyme reactor through the injection pump to carry out incubation; <18>O marking can be carried out at the same time with enzymolysis; and the <18>O marked peptide fragment after enzymolysis is enriched on-line and transferred into the polypeptide separation column for further separation, and finally is detected by the mass spectrometry detector. The <18>O on-line marked protein quantitative analysis platform integrates the protein on-line enzymolysis, <18>O on-line marking and polypeptide separation.

The invention discloses a scribing device, which comprises a transverse H-shaped pedestal, a sliding rod and a slide block, wherein a supporting plate is arranged in a square hole of the slide block; a scale plate is arranged in a groove; a circular penholder penetrates through a penholder penetration hole; an annular pad on the lower part of the circular penholder is arranged on a Z-shaped limiting block; a spring socket is sleeved on the lower part of the penholder to be arranged between the annular pad and the slide block; and a refill is connected with the circular penholder and is arranged on one plane with paper and a board. The transverse H-shaped pedestal is placed at an initial position where scribing is required, the sharp indicating head is adjusted to point at a corresponding scale, and a set screw is screwed up to fix the slide block on the supporting plate. A clamping head is pulled by one hand to draw one or more parallel lines with equal or unequal spaces on the paper, a wood board and the like. The scribing device has the advantages of simple structure, convenience and quickness in operation, stable and reliable work, capability of simultaneously drawing a plurality of parallel lines with equal or unequal spaces, short scribing time and capability of greatly improving the scribing accuracy and the working efficiency.

The invention provides a cis-platinum complex, and also relates to a synthetic method of the cis-platinum complex and an application of the cis-platinum complex in preparing nucleotide and protein probes. The cis-platinum complex can be connected with fluorescein, and can also be firmly connected with DNA, RNA and proteins, so that the cis-platinum complex can be used as a connection main body of the biological probe and is suitable for marking and detecting the target DNA, RNA and protein; the cis-platinum complex is simple in marking operation procedures, and is not limited to the size of a marking molecule and not limited to a pH value of a marking mixed system; the cis-platinum complex can be widely applied to the subdivisions such as nucleotide, protein (antibody) and protein micro arrays, hybridization of comparative genome and the like; in addition, the marking efficiency is significantly improved, the marking time is shortened, the fluorescent intensity stability is improved, and the marking effect is excellent; and therefore, the application prospect in the fields such as preparation of nucleotide and protein probes, research and development of biological medicine, diagnosis of diseases and the like is good.

{0><}0{>The invention discloses a radio-labeled tumor developing agent as well as a preparation method and application thereof. <0}{0><}0{>The radio-labeled tumor developing agent structurally contains an alpha-amino acid derivate structure capable of labeling nuclide and a tumor-targeted folic acid molecule, wherein radionuclide is 18F, and the alpha-amino acid derivate structure and the folic acid molecule are connected in a specific chemical mode. <0}{0><}0{>The invention further relates to a preparation method of the compound and application of the compound serving as a tracing developing agent in PET development in a human body or an animal body. The radio-labeled tumor developing agent has the advantages of simplicity in preparation, low price and strong targeting property especially when serving as a tumor developing agent.

The invention discloses a method for marking marine shellfishes by means of waterproof labels. The method comprises the following steps of 1 punching place selection and cleaning, 2 punching and recreation, 3 label printing and piercing and 4 marking. The method is high in label retention rate, obvious in marking, easy to operate, low in price and small in shell damage.

The invention provides a novel carbazole fluorescent thiol marking reagent as well as a synthesis method and application thereof. The florescent marking reagent adopts carbazole as a fluorescent basering and activated C=C double bonds as reaction activity groups, is simple and convenient in synthesis step, easy to operate and can be synthesized in a large scale through two steps of reactions as follows: (1) enabling carbazole to react with 4-bromobenzaldehyde so as to obtain an intermediate (4-(9H-carbazole-9-yl) benzaldehyde; (2) enabling the intermediate (4-(9H-carbazole-9-yl) benzaldehydeto react with malononitrile so as to obtain a target product (4-(9H-carbazole-9-yl) benzaldehyde malononitrile, and performing recrystallization for three times with acetonitrile, thereby obtaining ayellow needle-shaped crystal. The chemical purity of the fluorescent marking reagent provided by the invention is up to 99%, and the fluorescent marking reagent is stable in chemical property. The novel carbazole fluorescent thiol marking reagent provided by the invention is capable of rapidly and accurately marking degradation thiol products of organic thiol ester insecticides under a gentle condition, and quantitative analysis on contents of organic thiol ester insecticides in reagent samples can be achieved.

The invention discloses an EMSA method, a probe thereof and a preparation method of the probe. The EMSA probe comprises a first strip chain, and the first strip chain comprises a transcription factor combination sequence and joint sequences located at the 5' end and the 3' end of the transcription factor combination sequence. The preparation method of the EMSA probe comprises the following steps of synthesizing a template strand and a primer which is labeled through biotin and modified through LNA, wherein the template strand contains the transcription factor combination sequence and the joint sequences located at the 5' end and the 3' end of the transcription factor combination sequence, each joint sequence is a palindromic sequence, and the nucleotide sequence of the primer is the same as the nucleotide sequence of each joint sequence; adopting the primer for conducting PCR amplification with the template strand as the template and synthesizing the EMSA probe . The EMSA probe can be used for conducting EMSA. According to the EMSA method, the probe thereof and the preparation method of the probe, the double-stranded probe is prepared from a single-strained probe through PCR, false negativeness due to incomplete renaturation is avoided, and developing errors caused by the single-stranded free probe are further overcome.

The invention relates to an artificial intelligence technology, is applied to a smart city, and provides a method and device for acquiring a KPI abnormal data sample, computer equipment and a storagemedium. The method comprises the following steps: acquiring KPI data in a preset time period; carrying out anomaly detection on the KPI data to obtain a potential abnormal data point, and backtrackingand intercepting specified segment of KPI data as candidate KPI abnormal data according to a time sequence by taking the potential abnormal data point as an end point; adjusting the candidate KPI abnormal data and the known KPI abnormal data to enable the time lengths of the candidate KPI abnormal data and the known KPI abnormal data to be consistent, and performing similarity distance calculation on corresponding data points of the candidate KPI abnormal data and the known KPI abnormal data to obtain a plurality of regular path distances; judging whether the candidate KPI abnormal data are KPI abnormal data or not according to the regular path distances; and if so, marking the candidate KPI abnormal data as KPI abnormal data to serve as a sample for training the intelligent KPI abnormalrecognition model. A large number of KPI abnormal data samples are obtained through a small number of known KPI abnormal data samples, so that the operation and maintenance cost are greatly reduced.

The invention provides a dimeric amido benzimidazole compound. The structure of the dimeric amido benzimidazole compound is shown as a formula (I), wherein R0 is an integer of n from 0 to 5. The invention also provides a compound with anti-tumor activity and a radionuclide labeled compound targeted by interferon stimulating protein, wherein the compound is based on the dimeric amido benzimidazole compound. The dimeric amido benzimidazole compound has high affinity and high specificity to interferon stimulating protein, the compound with anti-tumor activity has the characteristic of activating an STING pathway in vitro, and the radionuclide labeled compound has the advantages that target uptake and non-target uptake are obviously compared, and the lipid solubility can be changed by introducing nuclides with different properties so that the application scene is greatly widened, and the compound can be combined with treatment nuclides for tumor treatment, and even can be used for immunotherapy.

The invention provides novel 18F labeled substituted benzimidazole compounds, which are characterized in that: one end of each compound contains an 18F substituted alkoxy structure, and the other end contains a 6-carboxyl / H benzimidazole structure; a substituent group R1, located on the site 2 of benzimidazole matrix, is hydrogen, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, benzyl, 2-methylthio ethyl and phenyl; a substituent group R2, located on the site 4 of benzimidazole matrix, is hydrogen, methyl and ethyl; and the structure of the compound is shown in formula A, wherein n is between 1 and 5, R2 is H, Me and Et, X is COOH and H. Experiments show that the compounds have high bioactivity such as fast serum removal, high serum stability and relatively low intake in tissues or organs such as liver and relatively high enrichment and slow removal rate in tumor cells, and therefore the compounds have relatively high tumor / background value and are favorable for PET tumor imaging. Meanwhile, the labeled precursor of the compounds is easy to synthesize and the labeling rate is extremely high; and such advantages indicate that the compounds have the tremendous potential to become PET tumor imaging agents.

The invention discloses a two-dimensional palladium-based probe with radioiodine labeling and a preparation method and application of the probe. The probe comprises a two-dimensional palladium-based nanomaterial, a PEG linking molecule, a targeting group R and radioiodine, a thiol group is arranged at one end of the PEG linking molecule, a modifiable group is arranged at the other end of the PEG linking molecule, one end of the PEG linking molecule is linked to the two-dimensional palladium-based nanomaterial through the above thiol group, the other end is linked to the targeting group R through the modifiable group, and radioiodine is directly labelled on the two-dimensional palladium-based nanomaterial. According to the present invention, a plurality of radioiodine can be carried on a unit of nanocarrier based on the strong binding force between the two-dimensional palladium-based nanomaterial and halogen ions, the probe has the characteristics of strong labeling ability, short labeling time, high labeling yield and no need of subsequent purification before application, and is more conducive to commercial application and clinical promotion of markers.

The invention belongs to the technical field of positron emission computed tomography (PET) imaging, and particularly relates to a method for rapidly marking Cys-Annexin V through <18>F and application of obtained <18>F-Al-NOTA-Cys-Annexin V in the aspect of detecting cell apoptosis. According to the method for rapidly marking Cys-Annexin V through <18>F, <18>F-Al-NOTA-Maleimide is adopted to mark Cys-Annexin V for the first time, the marking time used by the marking method is short, the marking rate is high, the fact of using <18>F for positioning and marking Cys-Annexin V is achieved, and <18>F-Al-NOTA-Cys-Annexin V maintains good affinity with PS, has a good imaging effect by serving as a PET imaging agent and has the more ideal imaging specificity.

The invention discloses 18F-E[c(RGDyk)2], a medicine box used for automatic production thereof, and a preparation method and use of the medicine box, relates to a polyethylene terephthalate (PET) developer and a preparation method and use thereof and relates to the technical field of radiative medicines and nuclear medicines. The medicine box contains a raw material reagent A and a raw material reagent B. A bottle A contains E[c(RGDyk)2]-NO2; and A bottle B contains acetonitrile solution of crown ether K222 / K2CO3. The 18F-E[c(RGDyk)2] prepared by the medicine box has high absorbing performance and excellent retaining performance in model mouse tumor, and has a very high target / non target ratio, excellent pharmacokinetic characteristic and high biological performance, and can completely meet the requirements of PET developer of tumour integrin alphavbeta3 receptor.

The invention relates to the field of machining, and discloses a limited angle torque motor mechanical zero position marking method which is easy to operate, accurate in marking and positioning and capable of greatly reducing marking time, and comprises the steps that S1, arranging a first shaft sleeve, a second shaft sleeve and a pointer at the output shaft end of a limited angle torque motor according to use requirements, arranging an eccentric small ball at the end part of an output shaft of the limited angle torque motor, wherein the eccentric small ball and the output shaft of the limited angle torque motor are integrated, and then carrying out calibration; and S2, starting the limited angle torque motor, firstly controlling the limited angle torque motor to rotate forwards, at the moment, driving a pointer to rotate to a limit angle position at one end by the limited angle torque motor, marking along one side by using a marking pen, then controlling the limited angle torque motor to rotate backwards, and marking along the other side by using the marking pen. And the marked motor finds an angular bisector on the milling machine according to the two lines, and a final marking line is carved, wherein the marking line is the mechanical zero position of the motor.

The invention provides a somatostatin analogue and application thereof. The structure of the somatostatin analogue is as shown in formula I), a Phe-D-Trp-Lys-Thr motif with high affinity to a somatostatin receptor is reserved in the structure of the somatostatin analogue, the probe structure is modified through different chelating agents and different nuclides, and a broad-spectrum radiolabeled somatostatin molecular probe targeting a somatostatin receptor is designed. All subtypes of somatostatin receptors are targeted, and the probe is used as an early diagnosis probe for broad-spectrum neuroendocrine tumors. Therefore, precise medical treatment of early diagnosis, early discovery and early treatment of neuroendocrine tumors is realized.

The invention discloses a novel protein biotin ligase and a proximity labeling system PhastID based on the novel protein biotin ligase. The invention specifically discloses a protein biotin ligase BPL, a modified mutant protein biotin ligase BPL * and an adjacent protein labeling system PhastID based on the biotin ligase BPL or BPL *. Compared with a traditional BioID proximity labeling system, the labeling system containing the protein biotin ligase BPL * has the advantages that the labeling intensity is higher, the required labeling time is shorter, and the application prospect is larger.

The invention provides a game role information processing method and device, equipment and a storage medium and relates to the technical field of games. The method comprises the following steps of in response to a selection operation for a target virtual character in a plurality of virtual characters displayed in a graphical user interface, providing an editing control corresponding to the target virtual character by the graphical user interface; in response to an editing operation through the editing control, determining the mark information of the target virtual character according to a touch position and / or a touch distance corresponding to a touch point of the editing operation; and recording the mark information. Compared with the prior art, a problem that the game experience of players and users is affected due to the fact that single recording time is long in a character input mode is solved.

The invention provides an RGD polypeptide coordination complex of a radioactive nuclide label. According to the structure of the coordination complex, the radioactive nuclide is selected from 64Cu, 68Ga, 111In, 62Cu, 67Cu, 67Ga, 86Y, 89Zr or 18F; the ligand is a ligand compound containing an RGD structure, which is shown in a formula (I). The coordination complex has stronger ligand stability and high target / non-target ratio, and the appetency of the ligand with integrin AlphavBeta3 is stronger. The invention further provides a preparation method of the coordination complex, and an application of the coordination complex in preparing tumor developers.

The invention provides a manufacturing process of a yin-yang board, and is characterized in that the manufacturing process comprises the following steps: 1) performing engineering evaluation of whether to design a yin-yang board and whether the yin-yang board has hidden quality danger if spliced; 2) drawing a yin-yang board splicing figure; 3) a PCB manufacturer confirming the jointed board, and requiring the PCB manufacturer to perform sample making, trial production and quality assessment; 4) performing batch production of yin-yang boards; 5) the processing technology of the yin-yang board including the steps of printing, surface mounting, reflow soldering, and AOI. The PCB can be obtained by trimming the yin-yang boards processed by the technology of the invention. Each time of production can reduce SMT line change time by 1H (change to the side B after the production of the side A is completed); surface mounting time of each side can be reduced by 4 seconds of Mark irradiation time(time for irradiation of 2 Marks); the side A and the side B are together, more elements can be mounted on the surface, so that it is more convenient to optimize the 8 elements of the mounting machine for one-time material collection (the mounting machine has 8 suction tubes in a group), and the number of material collection and the number of mounting round trips are reduced; therefore, production of each side can reduce mounting time by 4 seconds; and after changing to the yin-yang board, the overall production capacity of SMT is increased by about 30%.

The invention discloses a preparation method of a gold immunochromatography assay test strip indirectly connecting colloidal gold using a goat-anti-mouse secondary antibody with an anti-mouse marker.The preparation method comprises the following steps: (1) preparing a colloidal gold solution by using trisodium citrate to reduce chloroauric acid; (2) using the colloidal gold solution to mark the goat-anti-mouse secondary antibody; (3) adding the to-be-marked mouse antibody into the colloidal gold solution marking the goat-anti-mouse secondary antibody; (4) processing the indirectly marked mouse antibody colloidal gold solution onto a conjugate pad; (5) processing a corresponding antigen onto a nitric acid cellulose membrane; (6) adhering the nitric acid cellulose membrane, water absorptionpaper, the conjugate pad and a sample pad to a PVC plastic bottom plate; and (7) identifying the quality of the prepared gold immunochromatogrpahy assay test strip according to the product quality standard. The preparation method has the advantages that the detection sensitivity of the gold immunochromatogrpahy assay test strip can be improved, the consumption of antibodies used by the marker canbe reduced, the cost is reduced, the performance of the test paper is improved, and the detection result is stable and reliable.

The present invention relates to carbazole fluorescent thymine drug labeling reagent, synthesis and application. Carbazole is used as fluorescent mother ring, benzyl chloride is used as reactive group, and its chemical name is: 4-(9H-carbazole-9- base) benzyl chloride. The novel stable carbazole-based fluorescent thymine drug labeling reagent described in the present invention can accurately, sensitively and rapidly label thymine drugs under mild conditions, thereby realizing the separation of micro and trace amounts of thymine drugs in complex systems It can be used in research fields such as environmental analysis and food safety, and has broad application prospects.