A method for preparing colloidal gold-labeled immunochromatographic test strips using goat anti-mouse secondary antibody and indirectly linking mouse anti-labeling

Abstract

The invention discloses a preparation method of a gold immunochromatography assay test strip indirectly connecting colloidal gold using a goat-anti-mouse secondary antibody with an anti-mouse marker.The preparation method comprises the following steps: (1) preparing a colloidal gold solution by using trisodium citrate to reduce chloroauric acid; (2) using the colloidal gold solution to mark the goat-anti-mouse secondary antibody; (3) adding the to-be-marked mouse antibody into the colloidal gold solution marking the goat-anti-mouse secondary antibody; (4) processing the indirectly marked mouse antibody colloidal gold solution onto a conjugate pad; (5) processing a corresponding antigen onto a nitric acid cellulose membrane; (6) adhering the nitric acid cellulose membrane, water absorptionpaper, the conjugate pad and a sample pad to a PVC plastic bottom plate; and (7) identifying the quality of the prepared gold immunochromatogrpahy assay test strip according to the product quality standard. The preparation method has the advantages that the detection sensitivity of the gold immunochromatogrpahy assay test strip can be improved, the consumption of antibodies used by the marker canbe reduced, the cost is reduced, the performance of the test paper is improved, and the detection result is stable and reliable.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents in the field of biomedicine, more specifically, it relates to a preparation method of a gold-labeled immunochromatographic test strip in which colloidal gold is used to label a goat anti-mouse secondary antibody and then indirectly connected to a mouse anti-label. Background technique [0002] In 1971, Faulk and Taytor introduced colloidal gold into immunochemistry, and since then immunocolloidal gold technology has been used as a new immunological method. Colloidal gold labeling is essentially a coating process in which polymers such as proteins are adsorbed to the surface of colloidal gold particles. The adsorption mechanism may be that the negative charge on the surface of the colloidal gold particles forms a firm bond with the positively charged groups of the protein due to electrostatic adsorption. Various colloidal gold particles with different particle sizes, that is, ...

AI Technical Summary

Problems solved by technology

[0004] Colloidal gold immunochromatography technology has been widely used in the field of in vitro diagnostic technology, but most of the traditional colloidal gold labeling technology is to directly label the antibody to be labeled on the colloidal gold solution, which consumes too much antibody and the labeling method is complicated, thus affecting the colloidal gold. Development and popularization of gold immunochromatography technology