38results about How to "Short detection process" patented technology

The invention belongs to an infrared dim-small target detection method based on shear wave conversion in the infrared image processing technology. The method comprises the following steps: processing an original infrared image by employing nonsubsampled Laplace pyramid conversion and a Shearlet filter in succession to obtain high frequency information graphs of various directions under different scales, inhibiting background and noise interference information, enhancing target information and extracting a dim-small target. According to the invention, the nonsubsampled Laplace pyramid conversion and a Shearlet filter are employed to process the original infrared image, through same scale and different scales fusion processing of the obtained the high frequency information graphs, the interference information is inhibited, the target information is enhanced, and the high frequency information graphs are subjected to segmentation to obtain a clear dim-small target graph; thereby the method has the characteristics of a short detection processing flow, small data processing amount, short processing time, capability of effectively raising performance of detecting the infrared dim-small target and obviously distinguishing a target and a complex background in the image, a good effect and the like.

The invention provides a method for inspecting the acceptability of amorphous alloy products by means of sound, which includes the following specific steps: an amorphous alloy product is impacted to vibrate and emit sound under the condition of no contact with other objects; the sound signals of the amorphous alloy product, which are generated by impact, are acquired; the sound signals are processed, and the attenuation time of the sound signals is counted; and according to the length of the attenuation time of the sound signals, the acceptability of the product is judged. The method can be used for accurately inspecting whether shrunk holes, cracks or other defects exist in the amorphous alloy product, inspection can be automatically carried out as long as the threshold of the attenuation time of the sound signals of an acceptable product is set in advance, human subjective judgement is not needed, so that misjudgement can be reduced, moreover, the inspection flow is short, and the method is suitable for the inspection of products in large batches.

The invention discloses a method for detecting the dispersibility of titanium white in plastics, and belongs to the field of chemical industry. In order to solve the technical problem, the invention provides a simple and reliable method for detecting the dispersibility of titanium white in plastics. The method comprises the following steps of: a) fully mixing the titanium white and a resin to obtain a material to be detected; and b) extruding the material to be detected in an extruder, burning the material in different extrusion sections at resin decomposing temperature, calculating the solid content of the material in different extrusion sections, and characterizing the dispersibility of the titanium white in the plastics by using a ratio of the solid content, wherein the solid content is a quotient obtained by the mass of the material before burning by the mass of the material after burning. The dispersibility of the titanium white in the plastics is characterized by the ratio of the solid content, and the method has the advantages of high operability, simplicity, practicability, short detection flow, and high detection accuracy and suitable to be promoted and applied in the field.

The invention relates to the field of molecular biology, in particular to a nucleic acid signal amplification sequence and a nucleic acid signal amplification method. The nucleic acid signal amplification sequence comprises a leader sequence and a repetitive sequence in a plurality of times; the leader sequence and an objective target drone sequence complement each other; and the repetitive sequence and a reporter probe complement each other. An objective target drone can be captured and hybridized by the leader sequence of the nucleic acid signal amplification sequence, and then by the repetitive sequence in a plurality of times and corresponding reporter probes, signals can be amplified, so that nucleic acid signals of the objective target drone are detected. Compared with a PCR technology which needs enzyme to maintain a hybrid system, the nucleic acid signal amplification sequence can detect nucleic acid substances on the premise of not increasing concentration of a detected object, and is good in stability, high in repeatability, low in cost and short in detection technological process.

The invention provides a kit for detecting mitochondrial DNA T4353C mutation linked to primary hypertension. The kit is composed of DNA extraction mixed liquor, PCR mixed liquor, outer primers and inner primers specifically designed for T4353C, restriction endonuclease, a positive control, a negative control and a kit body. According to the invention, genomic DNAs are rapidly extracted from samples like a small amount of a blood specimen, hair with hair follicles, a mouth mucosa scraping smear and saliva by using a protease K digestion cleavage process, so problems in extraction of DNAs from blood of a patient with primary hypertension in the prior art are overcome, pains of a detected person are alleviated, and trans-regional sample transmission is realized; different sites formed by the specific restriction endonuclease enable specificity of enzyme digestion to be improved; the phenomenon of false negative results is avoided, and stability of detection results is improved; and the kit has the advantages of low detection cost, simple and fast detection flow, visually interpretable results and good practicality.

The invention provides a kit for detecting mitochondria DNA A4263G related to hypertension. The kit consists of a primer sequence, a reagent for extracting a sample genome DNA, a polymer chain reaction (PCR) amplified reaction reagent, a positive sample contrast, a negative sample contrast and an operating manual. According to the kit, the genome DNA is extracted from a small amount of samples (such as a venous blood drop from blood capillary, hair with hair follicle, mouth mucosa blade scraping or spit) by a proteinase K digestion lysis method; segmental nested PCR amplification is performed by the design of primers; and the mitochondria mutation sites related to the hypertension are detected by specific restriction enzyme styI enzyme cutting identification. The kit implements non-invasive detection, is more fast, economic, simple and convenient than the separate detection for the mitochondria DNA A4263G mutation, has low requirement on the equipment and environment, and is favorable for popularization and application and suitable for the extensive screening and preventive inspection for the related population of hypertension.

The invention provides a gene detection method of Leber's hereditary optic neuropathy, (LHON), a gene chip and a kit. The kit comprises a plurality of PCR probes and primers for detecting SNP of optic nerve lesion genes. The chip is provided with detection probes aiming at 13 mitochondrial genes and 46 SNP loci. According to the gene detection method, the kit and the gene chip are utilized to perform detection, the gene detection method comprises the following steps: performing amplification and hybridization on marked samples; scanning hybridization signals; obtaining chip data, and processing the obtained chip data; judging SNP loci, and judging and reading SNP loci information of the visual lesion genes. The gene detection method disclosed by the invention overcomes the detects that by adopting the direct sequencing, the using sample volume is large and the blood sample quantity is large, the amplification time is long, the amplification frequency is high, and false positive or false negative results can be easily caused, and the like, so that the pain of detected persons can be relieved, and not only be the detection accuracy improved, but also the detection time is shortened. According to the invention, the chip and the kit which have the characteristics of being rapid and being high in flux are provided, so that the early diagnosis and treatment of diseases can be facilitated, and nationwide large-scale screening and preventive inspection are convenient to carry out.

The invention relates to a rapid simultaneous detection method for the content of copper, zinc and iron in gold mud. A sample is dissolved by utilizing nitric acid, aqua regia, sulfuric acid and nitric-sulfuric acid; after sulfuric acid is added for smoking, a copper-zinc-iron compound is decomposed to form a corresponding oxide, HAuCl4 is decomposed to form elemental gold, and H<n-1>[AgCln]<-n+1> is decomposed to form silver chloride; after sulfuric acid is added for dissolving salts, the copper iron zinc oxide is dissolved to form sulfate; after ammonia water is added, silver-copper-zinc is transformed into corresponding ammonia complex ions, iron is transformed into iron hydroxide precipitation, and gold and iron are filtered and then separated from filtrate; after the pH value of the filtrate is adjusted by adding sulfuric acid and ammonia water, sodium chloride is added for precipitation of silver, the silver is recovered by filtering, copper in the filtrate is detected by adopting iodometry, and zinc is detected by adopting an EDTA method; and iron is detected by using a potassium dichromate method. The sample consumption is less in a detection process, precious metals can be effectively classified and recovered, the detection process is simplified, the energy consumption is reduced, and the detection efficiency is improved.

The invention provides a kit for simultaneously detecting mutations in mitochondria DNA A1555G and C1494T related to maternally inherited drug-induced deafness and a using method thereof. The kit comprises a reagent for extracting sample genomic DNA, a PCR amplification reactive reagent, a primer mixed liquor, a positive control template and a negative control template. The using method of the kit mainly comprises the following steps: using blood, hair with follicle, oral mucosa doctor blade, saliva, and the like, as a sample; adopting a proteinase K digestion pyrolysis method to extract genomic DNA; and then simultaneously detecting mutations in A1555G and C1494T by multiple allele specific PCR. The kit is used for detecting the mutations in mitochondria DNA A1555G and C1494T related to maternally inherited drug-induced deafness and is more rapid, economical and simpler than the single detection for mutation in A1555G or C1494T, and the kit has low requirements for equipment and environment and is conducive to promotion and application.

The invention provides a kit for detecting deaf related mitochondrial DNA T7505C mutation. The kit is composed of a DNA extraction mixed liquid, a PCR mixed liquid for T7505C fragment amplification, a pair of outer primers designed against T7505C, a pair of inner primers designed against the T7505C, a restrictive endonuclease, a positive contrast, a negative contrast and a kit body. A protease K digestion cracking method is utilized in the invention to rapidly extract genome DNA from a small amount of specimens, so a problem of the traditional extraction of DNA from the blood of a deaf patient is solved, the pains of a detected person is mitigated, and a trans-regional sample transmission problem is also solved. The kit has the advantages of low application cost, simple and rapid detection flow, visual result interpretation, ensuring of the specificity and the stability of the detection result, and realization of the application in the detection of the deaf related mitochondrial tRNAGlnT7505C mutation.

The invention provides a mitochondrial T3866C detection kit of Leber disease, and an application thereof. The detection kit is composed of a DNA extraction mixed solution, a PCR mixed solution used for amplifying T3866C, outer primers and inner primers designed aiming at T3866C, a restriction endonuclease Bgl II, a positive control, and a negative control. According to the invention, genomic DNA is rapidly extracted from small amounts of samples such as blood samples, hair with follicle, oral mucosa smears, or saliva. Therefore, a problem of DNA extraction in primary Leber disease patient blood of traditional methods is solved, pain of patient to be detected is reduced, a problem of cross-region sample transferring is solved, enzyme digestion specificity is improved, false negative is avoided, and detection result stability is improved. The kit provided by the invention is rapid, simple, accurate, and economical. With the kit, primary-Leber-disease-related mtDNAT3866C mutation large-scale screening and preventive inspection can be implemented.

The invention provides a kit for detecting mitochondrial DNA T5655C mutation linked to primary hypertension. The kit is composed of DNA extraction mixed liquor, PCR mixed liquor, outer primers and inner primers specifically designed for T5655C, restriction endonuclease, a positive control, a negative control and a kit body. According to the invention, genomic DNAs are rapidly extracted from samples like a small amount of a blood specimen, hair with hair follicles, a mouth mucosa scraping smear and saliva by using a protease K digestion cleavage process, so problems in extraction of DNAs from blood of a patient with primary hypertension in the prior art are overcome, pains of a detected person are alleviated, and trans-regional sample transmission is realized; different sites formed by the specific restriction endonuclease enable specificity of enzyme digestion to be improved; molecular internal control indexes are set, so the phenomenon of false negative results is avoided and stability of detection results is improved; and the kit has the advantages of low detection cost, simple and fast detection flow, visually interpretable results and good practicality.

The invention relates to a single-particle-based LIBS and Raman spectrum aerosol online detection device, wherein the aerodynamic lens is used to focus the atmospheric aerosol particle sample into a collimated particle beam and screen the particle size, an oscilloscope, two continuous The laser and the corresponding photomultiplier tube together constitute a dual-beam caliper system. The first and second Nd:YAG lasers interact with particles to excite Raman spectroscopy and LIBS spectroscopy respectively. The element composition information in aerosol particles can be detected in real time through LIBS spectroscopy, and the characteristic spectral lines of each element belong to atomic spectral lines without overlapping interference. Raman spectroscopy studies the vibrational and rotational spectra of molecules to obtain structural information on aerosol particles and to obtain information on lattice vibrations of particle samples. The device of the invention is simple and compact in structure, has low requirements on instruments, only needs a low vacuum environment to eliminate background noise interference, and reduces the cost of the detection device. The detection process of the device of the invention is short, and the analysis result can be obtained after a single pulse laser.

The invention aims to provide a kit for detecting mutation of mitochondria T12201C related with epicophosis, and the kit consists of a DNA extraction mixed liquor, a PCR mixed liquor for amplifying T12201C segments, a pair of outer primers designed according to T12201C, a pair of inner primers designed according to T12201C, restriction enzyme, a positive control unit, a negative control unit and a kit body. By using a proteinase K digestion cracking method to extract the genomic DNA from a few samples, the kit disclosed by the invention not only solves the problem that the DNA is extracted from blood of an epicophosis patient traditionally, relieves the pain of the detected patient, but also solves the problem of sample transmission between different areas, not only improves the specificity of enzyme digestion but also ensures the specificity and stability of the results, not only lowers the detection cost but also realizes simplicity and quickness in operation, and can be applied to detection of mutation of mitochondria gene T12201C related with epicophosis.