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Mutation detection kit for Parkinson disease and leukoaraiosis and detection method thereof

A technology for Parkinson's disease and mutation detection, which is applied in biochemical equipment and methods, and the determination/inspection of microorganisms. Simple, time-consuming results

Inactive Publication Date: 2014-06-18
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Generally, agarose gel electrophoresis is commonly used in laboratories to display the results of RFLP, but due to its low resolution and cumbersome preparation, it is not conducive to the operation of large batches of samples

Method used

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  • Mutation detection kit for Parkinson disease and leukoaraiosis and detection method thereof
  • Mutation detection kit for Parkinson disease and leukoaraiosis and detection method thereof
  • Mutation detection kit for Parkinson disease and leukoaraiosis and detection method thereof

Examples

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Effect test

Embodiment 1

[0058] Example 1 Using the present invention to detect 200 cases of sporadic Parkinson's patients and 200 cases of sporadic leukoaraiosis patients.

[0059] 1. Test samples

[0060] All patients were independently diagnosed by two neurologists. The diagnostic criteria of Parkinson's disease were based on the UK Parkinson's Disease Bank (UKPBB), and the severity was based on the United Parkinson's Disease Rating Scale (UPDRS). CT findings of leukoaraiosis There are patchy or diffuse low densities in the white matter of the bilateral brain, with fuzzy edges showing a halo shape, CT value is lower than that of the white matter, and no enhancement. MRI shows iso- or hypointensity on T1-weighted images, and iso- or hypointensity on T2-weighted images. high signal.

[0061] 2. Genomic DNA Extraction

[0062] 2ml of peripheral blood was extracted and anticoagulated with ethylenediaminetetraacetate (EDTA). It is recommended to operate according to the instructions of the DNA extrac...

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Abstract

The invention discloses a mutation detection lit for Parkinson disease and leukoaraiosis and a detection method thereof, which belong to the field of molecular diagnosis. The kit is provided with a packaging box, two pairs of primer sequences, a PCR (polymerase chain reaction) amplification reaction reagent, an enzyme digestion reaction reagent, a positive standard substance and a negative standard substance. The kit is applied in detection for the related mutations rs2066842 and rs75932628 of Parkinson disease and leukoaraiosis. The detection method comprises the following steps: using software Primer 5.0 and software Oligo 7.0 for obtaining specific PCR amplification products containing rs2066842 and rs75932628 according to the sequences of gene CARD15 and gene TREM2 of human respectively; digesting the specific PCR amplification products by restriction enzymes BamHI and HhaI; and detecting whether the two sites are mutated and the mutation types according to the quantity and size of digestion segments and in combination of the capillary electrophoresis.

Description

technical field [0001] The invention belongs to the field of molecular diagnosis, and relates to a kit for detecting mutations related to Parkinson's disease and leukoaraiosis: rs2066842, rs75932628, and also relates to a kit for detecting mutations related to Parkinson's disease and leukoaraiosis: rs2066842, rs75932628 The method, and the application of the above method and the above kit in the detection of mutations related to Parkinson's disease and leukoaraiosis: rs2066842, rs75932628. Background technique [0002] With the aggravation of aging, the prevalence of senile diseases is increasing, and Parkinson's disease and leukoaraiosis are two common senile diseases. Among elderly patients over 65 years old, the incidence rate of men is 1.6%, and the incidence rate of women is 1.7%. Among the 4 million patients in the world, 1.7 million people are in China, and the prevalence rate is close to that of European and American countries. This report was published in The Lance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/686C12Q1/6883C12Q2600/156C12Q2535/138C12Q2565/125
Inventor 曾骥孟李志明顾龙安兴凯林青
Owner XIAMEN UNIV
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