The invention provides a high-quality, efficient and economical production method of strawberry plug seedlings. The method comprises the main technical measures that tissue culture virus-free seedlings are selected as mother plants, and soilless culture is adopted in mother plant cultivation, juvenile seedling breeding and cutting; hybrid and bad plants are removed in the mother plant fruiting period, and transparent and colorless film mulching is conducted after juvenile seedling cutting; reproductive growth of the mother plants is controlled, and nitrogen fertilizer supply is increased. Health, high quality and high production efficiency of the seedlings are guaranteed, and the dependence degree of the cutting link on facilities is decreased; the mother plants are subjected to field planting in autumn and subjected to breed seedlings in summer after fruiting in winter and spring, the mother plants can produce juvenile seedlings in summer, each mother plant can produce 60-70 juvenile seedlings in summer, the mother plants can produce fresh fruits in winter and spring, and 300-400 g of fresh fruits can be harvested from each mother plant; the production cost is effectively shared, the strawberry plug seedling production time is short, the cutting seedlings reach the field planting standard 40 days after cutting, the cutting survival rate is high and reaches 93% or above, high-quality, efficient and economical production of the strawberry plug seedlings is achieved, the practical value is high, and the method can be applied to practical production in a large scale.