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Method for culturing and producing micro-bulbodium of crocus sativus L. through superficial layer

A technology of shallow cultivation and saffron, applied in the biological field, can solve the problems of seedling production remaining in the experimental stage, high production cost, lack of germplasm resources, etc., and achieve short cultivation period, low cost, and transplant survival rate. high effect

Active Publication Date: 2015-08-26
江苏丰收大地种业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because saffron cannot reproduce sexually, it can only reproduce asexually through bulbs, and its bulbs degenerate seriously under cultivation conditions, resulting in a serious shortage of germplasm resources.
Since the introduction of the species in my country, the yield of cultivated saffron has always been low, which is far from meeting people's needs.
In recent years, tissue culture technology has been applied to the production of saffron seedlings, which provides a solution to the shortage and degradation of saffron bulbs. However, plant tissue culture is a labor-intensive industry, and the production cost remains high. The production of seedlings is also difficult. only in the experimental stage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for producing saffron microbulbs by shallow culture comprises the following steps:

[0030] (1) Sterilization of the culture container: put the culture medium into the culture container, and wait for use after high temperature and high pressure damp heat sterilization;

[0031] (2) Obtaining and sterilization of explants: put the saffron corm into water containing disinfectant and cultivate it until it grows leaves, remove the leaves, and rinse with water for 30 minutes; carry out surface disinfection on the above-mentioned saffron leaves in an ultra-clean bench Sterilization treatment; specific steps: soak in 75% alcohol for 15 seconds, wash with sterile water twice, soak in 0.1% mercury chloride for 3 minutes, wash with sterile water for 5 times;

[0032](3) The preparation of the explant of superficial culture: the blade that has been sterilized in step (2) is cut into the segment of 0.5cm under aseptic environment, and inoculates into the sterilized induc...

Embodiment 2

[0037] The method for producing saffron microbulbs by shallow culture comprises the following steps:

[0038] (1) Sterilization of the culture container: put the culture medium into the culture container, and wait for use after high temperature and high pressure damp heat sterilization;

[0039] (2) Acquisition and sterilization of explants: put the saffron corm into water containing disinfectant and cultivate until it grows leaves, remove the leaves, and rinse with water for 35 minutes; carry out surface disinfection of the above-mentioned saffron leaves in an ultra-clean bench Sterilization treatment; specific steps: soak in 75% alcohol for 15 seconds, wash with sterile water twice, soak in 0.1% mercury chloride for 4 minutes, wash with sterile water for 6 times;

[0040] (3) The preparation of the explant of superficial culture: the blade that has been sterilized in step (2) is cut into the segment of 0.7cm under aseptic environment, and is inoculated into the induced saffr...

Embodiment 3

[0045] The method for producing saffron microbulbs by shallow culture comprises the following steps:

[0046] (1) Sterilization of the culture container: put the culture medium into the culture container, and wait for use after high temperature and high pressure damp heat sterilization;

[0047] (2) Obtaining and sterilizing treatment of explants: put the saffron corm into water containing disinfectant and cultivate until it grows leaves, remove the leaves, and rinse with water for 40 minutes; carry out surface disinfection of the above-mentioned saffron leaves in an ultra-clean bench Sterilization treatment; specific steps: soak in 75% alcohol for 15 seconds, wash with sterile water for 4 times, soak in 0.1% mercury chloride for 5 minutes, wash with sterile water for 8 times;

[0048] (3) Preparation of explants of shallow culture: the sterilized leaves in step (2) are cut into 1.0cm segments under aseptic environment, and inoculated into the sterilized induced saffron callus...

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PUM

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Abstract

The invention discloses a method for culturing and producing micro-bulbodium of crocus sativus L. through a superficial layer. The method comprises steps: (1), sterilization of a culture container; (2), acquisition of an explant, and sterilization treatment; (3), preparation of the explant cultured on the superficial layer; (4), superficial layer cultivation, wherein the superficial layer cultivation stage comprises two processes of (A) cluster bud induction and (B) micro-bulbodium formation. A liquid culture mode is adopted for superficial layer cultivation, a culture body can more easily absorb nutritional ingredients in a culture medium, accordingly, seedlings can grow fast, and the production time of micro-bulbodium is shortened; meanwhile, the cultivation operation of the superficial layer is simple, the cost is low, and large-scale and industrial production is easier to realize.

Description

technical field [0001] The invention relates to a method for producing plant seedlings, in particular to a method for producing saffron microbulbs through shallow layer cultivation, and belongs to the field of biotechnology. Background technique [0002] Saffron (Crocus sativus L.), also known as saffron and saffron, is a perennial flower of the genus Crocus in the family Iridaceae, and is also a common spice. It is a native species in Southwest Asia and was first cultivated by the Greeks. It is mainly distributed in Europe, the Mediterranean, and Central Asia. It was introduced into China during the Ming Dynasty. The "Compendium of Materia Medica" listed it as a medicine, and it is planted in Zhejiang and other places in China. It is a precious Chinese medicinal material with strong physiological activity. Its stigma is used as medicine in Asia and Europe. It has sedative, expectorant and antispasmodic effects. It is used for stomach problems, menstruation regulation, meas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 周志疆陈明亮张丽丽韩婷
Owner 江苏丰收大地种业发展有限公司
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