Method for efficiently propagating Chinese ash by tissue culture

Abstract

The invention discloses a method for propagating Chinese ash by tissue culture, which comprises the steps of: with an excellent coppice shoot at the root of a single Chinese ash as an explant, inoculating in a culture bottle containing a starting culture medium after disinfected; with a common fluorescent lamp as a light source, illuminating for 16h every day, culturing for 45 days at a temperature of 22-25 DEG C and humidity of 50-65 percent, differentiating and growing into a test tube bud; shearing, transplanting into a culture bottle containing a multiplication culture medium for multiplication culture, carrying out continuous multiplication; shearing and then inoculating in a culture bottle containing a strong stock culture medium for strong stock culture; removing root callus and part of leaves after 25 days, remaining 3-4 leaves, inoculating in a culture bottle containing a root anlage culture medium generated through root processing for rooting culture for 6-10 days; and transplanting into a matrix with volume ratio of peat to yellow subsoil of 3:2 after cleaning, and rooting after 20 days and surviving. The rooting rate of the Chinese ash reaches above 89 percent, and the sprout quality is good.

Description

technical field [0001] The invention relates to a plant tissue culture propagation method, in particular to a tissue culture propagation method of maple poplar. Background technique [0002] Maple poplar is an important native tree species widely distributed in the middle and lower reaches of the Yangtze River in my country. It is a deciduous tree of the Juglandaceae family. The branches have flaky pith, and the bare buds have brown hairs on the outside. Leaves, the edges of the leaves have fine serrations, the fruit is nearly spherical, with two oblong or oblong-lanceolate fruit wings, 2 cm to 3 cm long, the flowering period is from April to May, and the fruit ripening period is from August to In September, Maple Yang likes light, likes a warm and humid climate, and is also relatively cold-resistant. It is not strict on the soil, has deep roots, obvious taproots, well-developed lateral roots, and strong germination. Its wood is light in color, light in weight, less warped, an...

AI Technical Summary

Problems solved by technology

[0003] Traditional maple poplar is propagated by seed sowing, cutting, and layering, but the speed of seed sowing and propagation in the above method is very slow, and because maple poplar has "injury flow" during the growth period, the propagation method of cutting and layering makes the survival rate very low. Moreover, the quality of the seedlings obtained is uneven. Due to the lack of excellent germplasm resources for breeding, it is difficult to obtain a large number of high-quality seedlings in a short period of time by traditional methods.