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Herba Dendrobii tissue cultured seedling medium and preparation method thereof

A technology for Dendrobium officinale and tissue culture seedlings is applied in the directions of botanical equipment and methods, gardening methods, gardening tools/equipment, etc., to achieve the effects of improving reliability, industrialized production efficiency, simple operation, and reducing production costs.

Inactive Publication Date: 2017-02-15
许焕明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The invention provides a culture medium of Dendrobium candidum tissue culture seedlings and a preparation method thereof, in order to solve the problems of how to increase the content of alkaloids, polysaccharides and amino acids in the main active components of Dendrobium candidum in the existing tissue culture medium, and improve economic benefits, etc.

Method used

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  • Herba Dendrobii tissue cultured seedling medium and preparation method thereof
  • Herba Dendrobii tissue cultured seedling medium and preparation method thereof

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preparation example Construction

[0071] The preparation method of described Dendrobium officinale tissue culture medium comprises the following steps:

[0072] S1: After peeling the watermelon, crush the watermelon with a grinder, and pass the obtained juice through a 3000-5000 mesh sieve to obtain watermelon juice;

[0073] S2: Watermelon juice, malic acid, o-benzoylsulfonimide, tea polyphenols, potassium salt, phosphorus salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt prepared in step S1 , zinc salts, manganese salts, copper salts, iron salts, vitamins, amino acids, indole butyric acid, gibberellins, 6-benzyl adenine, naphthalene acetic acid, kinetin, aspartate, cosolvents, dispersants , compatibilizer, and water are mixed, and stirred at a microwave power of 130-170W, a temperature of 56-61°C, and a rotation speed of 200-400r / min for 26-54min to obtain a mixed solution A;

[0074] S3: Add conditioner, stabilizer and antibacterial agent to the mixed solution A pr...

Embodiment 1

[0077] A culture medium for tissue culture seedlings of Dendrobium officinale, which comprises the following raw materials in parts by weight: 260 parts of watermelon juice, 3 parts of malic acid, 0.05 part of o-benzoylsulfonimide, 2.2 parts of tea polyphenols, and 3.5 parts of potassium salt 2.6 parts of phosphorus salt, 2.2 parts of nitrogen salt, 1 part of calcium salt, 1 part of magnesium salt, 0.5 part of iodine salt, 0.3 part of boron salt, 0.25 part of molybdenum salt, 0.35 part of zinc salt, 0.2 part of manganese salt, 0.15 part of copper salt 0.16 parts of iron salts, 0.2 parts of vitamins, 0.16 parts of amino acids, 0.1 parts of indolebutyric acid, 0.1 parts of gibberellin, 0.08 parts of 6-benzyl adenine, 0.12 parts of naphthaleneacetic acid, 0.08 parts of kinetin, asparagus 02 parts of Mensu, 0.22 parts of conditioner, 2.5 parts of co-solvent, 2 parts of dispersant, 1.6 parts of compatibilizer, 1.3 parts of stabilizer, 2 parts of antibacterial agent, 3500 parts of wa...

Embodiment 2

[0092]A culture medium for Dendrobium candidum tissue culture seedlings, in parts by weight, comprising the following raw materials: 150 parts of watermelon juice, 2.5 parts of malic acid, 0.03 parts of o-benzoylsulfonimide, 1.5 parts of tea polyphenols, and 2.6 parts of potassium salt 1.5 parts of phosphorus salts, 1.2 parts of nitrogen salts, 0.8 parts of calcium salts, 0.7 parts of magnesium salts, 0.4 parts of iodized salts, 0.2 parts of boron salts, 0.2 parts of molybdenum salts, 0.3 parts of zinc salts, 0.1 parts of manganese salts, and 0.1 parts of copper salts 0.1 part of iron salt, 0.12 part of vitamin, 0.1 part of amino acid, 0.08 part of indolebutyric acid, 0.08 part of gibberellin, 0.07 part of 6-benzyl adenine, 0.08 part of naphthaleneacetic acid, 0.05 part of kinetin, asparagus 0.14 parts of Mensu, 0.19 parts of conditioner, 1.6 parts of co-solvent, 1.4 parts of dispersant, 1.3 parts of compatibilizer, 1 part of stabilizer, 1.4 parts of antibacterial agent, 2800 p...

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Abstract

The invention discloses a Herba Dendrobii tissue cultured seedling medium and a preparation method thereof. The Herba Dendrobii tissue cultured seedling medium comprises the following raw materials: watermelon juice, malic acid, o-benzoic sulfimide, tea polyphenols, sylvite, microcosmic salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, molysite, vitamin, amino acid, indolebutyric acid, gibberellin, 6-benzyl adenine, naphthylacetic acid, kinetin, asparagine, a conditioner, a cosolvent, a dispersant, a compatilizer, a stabilizer, an antibacterial agent and water. Seedlings obtained by tissue culturing of Herba Dendrobii by the Herba Dendrobii tissue cultured seedling medium are good in quality and high in survival rate, and contents of alkaloid, polysaccharide and amino acid can be increased effectively.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of dendrobium candidum planting, and in particular relates to a medium for tissue culture seedlings of dendrobium candidum and a preparation method thereof. [0003] 【Background technique】 [0004] Dendrobium officinale is a plant of the orchid family. Dendrobium can be divided into dozens of species such as yellow grass, nobile, and horsewhip. The main active ingredients are alkaloids, polysaccharides, and amino acids. Dendrobium candidum is the best product of Dendrobium. It is named for its iron-green skin. It grows in the shade of cliffs, and its roots do not go into the soil. Fat, lower blood sugar, inhibit tumor, brighten eyes, nourish skin and prolong life. "Chinese Pharmacy Dictionary" states that Dendrobium candidum "specially treats the gas and liquid of the lung and stomach, the gas and liquid are prosperous, and the kidney water is self-generated." It is good at nourishing yin and promo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 许焕明
Owner 许焕明
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