Herba Dendrobii tissue cultured seedling medium and preparation method thereof
A technology for Dendrobium officinale and tissue culture seedlings is applied in the directions of botanical equipment and methods, gardening methods, gardening tools/equipment, etc., to achieve the effects of improving reliability, industrialized production efficiency, simple operation, and reducing production costs.
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[0071] The preparation method of described Dendrobium officinale tissue culture medium comprises the following steps:
[0072] S1: After peeling the watermelon, crush the watermelon with a grinder, and pass the obtained juice through a 3000-5000 mesh sieve to obtain watermelon juice;
[0073] S2: Watermelon juice, malic acid, o-benzoylsulfonimide, tea polyphenols, potassium salt, phosphorus salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt prepared in step S1 , zinc salts, manganese salts, copper salts, iron salts, vitamins, amino acids, indole butyric acid, gibberellins, 6-benzyl adenine, naphthalene acetic acid, kinetin, aspartate, cosolvents, dispersants , compatibilizer, and water are mixed, and stirred at a microwave power of 130-170W, a temperature of 56-61°C, and a rotation speed of 200-400r / min for 26-54min to obtain a mixed solution A;
[0074] S3: Add conditioner, stabilizer and antibacterial agent to the mixed solution A pr...
Embodiment 1
[0077] A culture medium for tissue culture seedlings of Dendrobium officinale, which comprises the following raw materials in parts by weight: 260 parts of watermelon juice, 3 parts of malic acid, 0.05 part of o-benzoylsulfonimide, 2.2 parts of tea polyphenols, and 3.5 parts of potassium salt 2.6 parts of phosphorus salt, 2.2 parts of nitrogen salt, 1 part of calcium salt, 1 part of magnesium salt, 0.5 part of iodine salt, 0.3 part of boron salt, 0.25 part of molybdenum salt, 0.35 part of zinc salt, 0.2 part of manganese salt, 0.15 part of copper salt 0.16 parts of iron salts, 0.2 parts of vitamins, 0.16 parts of amino acids, 0.1 parts of indolebutyric acid, 0.1 parts of gibberellin, 0.08 parts of 6-benzyl adenine, 0.12 parts of naphthaleneacetic acid, 0.08 parts of kinetin, asparagus 02 parts of Mensu, 0.22 parts of conditioner, 2.5 parts of co-solvent, 2 parts of dispersant, 1.6 parts of compatibilizer, 1.3 parts of stabilizer, 2 parts of antibacterial agent, 3500 parts of wa...
Embodiment 2
[0092]A culture medium for Dendrobium candidum tissue culture seedlings, in parts by weight, comprising the following raw materials: 150 parts of watermelon juice, 2.5 parts of malic acid, 0.03 parts of o-benzoylsulfonimide, 1.5 parts of tea polyphenols, and 2.6 parts of potassium salt 1.5 parts of phosphorus salts, 1.2 parts of nitrogen salts, 0.8 parts of calcium salts, 0.7 parts of magnesium salts, 0.4 parts of iodized salts, 0.2 parts of boron salts, 0.2 parts of molybdenum salts, 0.3 parts of zinc salts, 0.1 parts of manganese salts, and 0.1 parts of copper salts 0.1 part of iron salt, 0.12 part of vitamin, 0.1 part of amino acid, 0.08 part of indolebutyric acid, 0.08 part of gibberellin, 0.07 part of 6-benzyl adenine, 0.08 part of naphthaleneacetic acid, 0.05 part of kinetin, asparagus 0.14 parts of Mensu, 0.19 parts of conditioner, 1.6 parts of co-solvent, 1.4 parts of dispersant, 1.3 parts of compatibilizer, 1 part of stabilizer, 1.4 parts of antibacterial agent, 2800 p...
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