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Method for quickly propagating germchit of giantleaf ardisia rhizome through bud plumular axis

A technology of trombone and hypocotyl, applied in the field of plant biology, can solve problems such as no reports on research, and achieve the effects of broad application prospects, strong practical operability, and large output

Inactive Publication Date: 2015-05-06
罗定市百草源种养专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the research on Zoumatai focuses on the fields of biology, ecological resources and effective component analysis, but the research on the tissue culture technology of Zoumatu seedlings has not been reported yet.

Method used

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  • Method for quickly propagating germchit of giantleaf ardisia rhizome through bud plumular axis
  • Method for quickly propagating germchit of giantleaf ardisia rhizome through bud plumular axis
  • Method for quickly propagating germchit of giantleaf ardisia rhizome through bud plumular axis

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Experimental program
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Effect test

preparation example Construction

[0045] Preparation of culture medium: Prepare the culture medium according to conventional methods, adjust the pH value to 5.8, sterilize at 124°C for 25 minutes after aliquoting, and set aside.

[0046] Seed collection, processing and disinfection: The mature fruits with deep red peel and no damage from diseases and insect pests were collected from Zoumatai Germplasm Garden of Guangxi Institute of Botany. The pulp and seed coat were removed, cleaned with tap water, and full-grown seeds were selected. On the ultra-clean workbench, first soak it in an alcohol solution with a volume fraction of 70-75% for 30 seconds, then soak it in a sodium hypochlorite solution with a mass fraction of 15% for 10-15 minutes, and then rinse it with sterile water 5 times. Blot the moisture on the surface with sterile filter paper for later use.

Embodiment 1

[0048] Utilize the method for rapidly propagating its seedling of Zouma embryo hypocotyl, comprises the following specific steps:

[0049] (1) Select well-grown Zoumatai seeds, add gibberellin aqueous solution with a concentration of 120 mg / L after surface disinfection and sterilization, the volume ratio of the solution to the seeds is 5:1, at a temperature of 35±2°C and a rotational speed of Soak for 24 hours on a shaker at 130~138 rpm, and rinse with sterile water.

[0050] (2) Inoculate the treated seeds in the germination medium, the germination medium is: MS+6-BA 0.5 mg / L+NAA 0.1 mg / L, at the same time add agar powder 5 g / L, sucrose 30 g / L , 0.2% activated carbon, pH 5.8; culture conditions are dark culture at a temperature of 28±2°C. After 5-7 days of culture, the radicle germinates and elongates, and the hypocotyl elongates 2-3cm after 40-50 days, and the cotyledons unfold to form young shoots. The germination rate reaches 97.5%, see figure 1 .

[0051] (3) After th...

Embodiment 2

[0056] Utilize the method for rapidly propagating its seedling of Zouma embryo hypocotyl, comprises the following specific steps:

[0057] (1) Select well-grown Zoumatai seeds, add gibberellin aqueous solution with a concentration of 150 mg / L after surface disinfection and sterilization, the volume ratio of the solution to the seeds is 5:1, at a temperature of 35±2°C and a rotational speed of Soak for 24 hours on a shaker at 130~138 rpm, and rinse with sterile water.

[0058] (2) Inoculate the treated seeds in the germination medium, the germination medium is: MS+6-BA 1.0 mg / L+NAA 0.2 mg / L, at the same time add agar powder 5 g / L, sucrose 30 g / L , 0.2% activated carbon, pH 5.8; culture conditions are dark culture at a temperature of 28±2°C. The germination rate was 85.2% after 40 days of cultivation.

[0059] (3) After the seeds germinate, cut the immature hypocotyls and insert them into the adventitious bud induction medium to induce adventitious buds. The hypocotyl adventit...

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Abstract

The invention discloses a method for quickly propagating germchit of giantleaf ardisia rhizome through bud plumular axis. The method comprises the following steps: selecting fully-growing seeds of giantleaf ardisia rhizome; disinfecting and sterilizing the surfaces; processing through gibberellins; inoculating to a sprouting culture medium to induce to develop buds; cutting the bud plumular axis; introducing into an adventitious bud inducting culture medium to form adventitious buds; cutting the adventitious buds into small sections with 1 to 2 axillary bud; introducing into a bud propagation culture medium to propagate; cutting single bud which strongly growing and is 3 to 4cm in length; introducing into a rooting culture medium to induce to root; performing hardening-seedling and transplanting. The method has the advantages that few giantleaf ardisia rhizome materials are utilized to fast propagate a lot of adventitious buds; the survival rate is up to 82.5%; an effective way is provided for industrial seedling of giantleaf ardisia rhizome.

Description

Technical field [0001] The present invention belongs to the field of plant biotechnology, and it involves a method of rapid breeding of its seedlings with the use of young buds of germination of horses. Background technique [0002] Tire for the horse ( Ardisia Gigantifoliastapf ) It is commonly used by the people's decline damage. The rhizomes and the whole plant have the effects of removing wind and bone, promoting blood circulation and stasis, swelling and pain, and hemostasis., Half -body inconsistency, ulcer ulcer, etc.Modern studies have proved that the abortion of the horse is rich in three -sulfidoside, cabbage derivatives, and volatile oils. Among them, the ingredients of Sanxia Saponin have a strong inhibitory effect on a variety of tumor cell activity.Such as: Extracting Sicratamin A as the mother nucleus from the tire and dry root stems from the horse tire as the mother nucleus AG4, the lung cancer cell A549, the liver cancer cell BEL-7402, the human gastric adenocarc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01C1/00A01C1/08
Inventor 唐凤鸾黄宁珍何金祥赵健毛世忠
Owner 罗定市百草源种养专业合作社
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