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Stem tip detoxification tissue culture medium and stem tip detoxification tissue culture method applicable to heat treatment of dendranthema morifolium

A technology for stem tip detoxification and cut chrysanthemum, which is applied in the field of plant cultivation and can solve the problems of affecting the quality of cut flowers, the degradation of cut chrysanthemum varieties, and the inability of large-scale production of seedlings.

Active Publication Date: 2020-02-04
苏州胜景泰园林环境建设有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the current deficiencies in the degeneration of cut chrysanthemums and purple wild chrysanthemums, and the transplanting and planting technology of tissue cultured seedlings, the purpose of this invention is to provide a method for improving the efficient proliferation of detoxified tissue cultured seedlings suitable for heat treatment of cut chrysanthemums, and to solve the problem of Long-term asexual reproduction leads to a large accumulation of viruses in the plant, causing the degeneration of cut chrysanthemum varieties and weakening of growth potential, affecting the quality of cut flowers, and the problem that seedlings cannot be efficiently mass-produced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1:

[0048] The first object of the present invention is to form a method for efficient breeding of cut-flower chrysanthemum heat-treated stem tip detoxified tissue-cultured seedlings. The implementation site is the garden flower genetics and breeding laboratory of the Institute of Horticulture, Zhejiang Academy of Agricultural Sciences and the cut-flower chrysanthemum production base of Jiaxing Jiade Horticultural Co., Ltd., the above-mentioned The method includes the following steps:

[0049] (1) The preparation of the culture medium: including basic medium and the components of the culture medium at each stage of tissue culture, and the weight of each component per liter is:

[0050] a) Basic medium: where,

[0051] Shoot tip induction medium Ⅰ is 20g / L sucrose, 4.4 g / L agar powder, 1 / 2 B5+Ca(NO3) at pH 5.8 2 500mg / L medium;

[0052] Ordinary medium (explant primary induction, strong seedling medium and shoot tip induction medium II) is MS medium with 30 ...

Embodiment 2

[0071] The first object of the present invention is to form a method for efficient breeding of cut-flower chrysanthemum heat-treated stem tip detoxified tissue-cultured seedlings. The implementation site is the garden flower genetics and breeding laboratory of the Institute of Horticulture, Zhejiang Academy of Agricultural Sciences and the cut-flower chrysanthemum production base of Jiaxing Jiade Horticultural Co., Ltd., the above-mentioned The method includes the following steps:

[0072] (1) The preparation of the culture medium: including basic medium and the components of the culture medium at each stage of tissue culture, and the weight of each component per liter is:

[0073] a) Basic medium: where,

[0074] Shoot tip induction medium Ⅰ is 20g / L sucrose, 4.4 g / L agar powder, 1 / 2 B5+Ca(NO3) at pH 5.8 2 500mg / L medium;

[0075] Ordinary medium (explant primary induction, strong seedling medium and shoot tip induction medium II) is MS medium with 35 g / L sucrose, 4.4 g / L ...

Embodiment 3

[0094] In this example: the domesticated varieties of purple wild chrysanthemum were collected in Chun'an, Zhejiang Province and carried out tissue culture in Hangzhou. In mid-April 2017, 200 bottles of rooted tissue culture seedlings with a height of 7-10 cm were selected, with an average of 10 plants per bottle, and were carried out in Hangzhou Greenhouse. Transplanting test.

[0095] Specific operation steps: Wash the tissue-cultured seedlings with clean water, drain the water, and air-dry naturally. Use medicaments (50% carbendazim WP 600 times, 97% hymexazol 1700-2000 times, 80% mancozeb WP 400-600 times, 40% azoxystrobin 800-1000 times) disinfection configuration good substrate. The dried tissue-cultured seedlings were inserted into the treated peat substrate for cutting culture. After 1-2 months of growth, cut off the 7-10cm shoots and dip them in rooting powder. Mix the rooting coconut peat matrix with disinfectants (50% carbendazim WP 600 times, 80% mancozeb WP 400...

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Abstract

The invention discloses a stem tip detoxification tissue culture medium and a stem tip detoxification tissue culture method applicable to heat treatment of dendranthema morifolium, and belongs to thetechnical field of plant cultivation. The tissue culture medium comprises an induction medium combination, a stem tip detoxification induction medium combination, a seedling strengthening and multiplying medium combination and a rooting medium combination which are used sequentially in stages. The induction medium combination comprises an explant induction medium, an explant primary induction medium and an explant induction medium which are used in sequence. The stem tip detoxification induction medium combination comprises an explant induction medium, a stem tip induction medium I and a stemtip induction medium II which are used in sequence. The seedling strengthening and multiplying medium combination comprises a seedling strengthening medium and a multiplying medium which are used in sequence. The problems that a large amount of bacteria are accumulated in plant bodies due to long-term vegetative propagation, the variety degeneration and poor growth vigor of chrysanthemums are caused, the quality of the dendranthema morifolium is affected and seedlings cannot be produced efficiency and massively are solved.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, in particular to a detoxification tissue culture substrate and method suitable for heat treatment of cut chrysanthemum stem tips. Background technique [0002] At present, the cut chrysanthemums cultivated in our province are mainly summer chrysanthemums and autumn chrysanthemums. The harvest period of summer chrysanthemums is from June to July. The growth characteristics are in a warm environment, with a night temperature of 10°C-23°C and a day temperature of 23°C-30°C. Under the environment, it can show good growth characteristics of the variety, is not sensitive to photoperiod response, and can be cultivated under natural light, but it is not resistant to high temperature. When cultivated under high temperature in summer, it is easy to have thickened calyx, smaller flower diameter, curled and uneven petals, and even The phenomenon of inability to develop and bloom after budding, repr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 郭方其吴超袁峰黑银秀柴金甫刘君丁晓瑜徐智豪黎侠
Owner 苏州胜景泰园林环境建设有限公司
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