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Carya illinoensis tissue culture propagating method

A thin-shell hickory, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of no further multiplication, strong seedlings, rooting, etc., and achieve fast propagation speed, high survival rate, Good quality seedlings

Inactive Publication Date: 2012-03-28
JIANGSU ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, Fu Yulan and others have only made some progress in the disinfection of explants and obtaining sterile materials, and have not further carried out research on key technologies such as proliferation, seedling strengthening, and rooting.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Basic medium configuration:

[0047] Among them, the components of constant elements and their corresponding use concentrations are as follows:

[0048] Potassium nitrate (KNO3) 950mg / L, ammonium nitrate (NH4NO3) 825mg / L, potassium dihydrogen phosphate (KH2PO4) 85mg / L, magnesium sulfate (MgSO4 7H2O) 185mg / L, calcium chloride (CaCl2 2H2O) 165mg / L L;

[0049] The components of trace elements and their corresponding concentrations are as follows: potassium iodide (KI) 0.83mg / L, boric acid (H3BO3) 6.2mg / L, manganese sulfate (MnSO4 4H2O) 22.3mg / L, zinc sulfate (ZnSO4 7H2O) 8.6mg / L, sodium molybdate (Na2MoO4 2H2O) 0.25mg / L, copper sulfate (CuSO4 5H2O) 0.025mg / L, cobalt chloride (CoCl2 6H2O) 0.025mg / L;

[0050] The composition of the iron salt and its corresponding concentration are as follows: disodium ethylenediaminetetraacetic acid (Na2·EDTA) 37.3mg / L, ferrous sulfate (FeSO4.7H2O) 27.8mg / L.

[0051] The components of organic components and their corresponding concentration...

Embodiment 2

[0066] Basic medium configuration:

[0067] Among them, the components of constant elements and their corresponding use concentrations are as follows:

[0068] Potassium nitrate (KNO3) 1900mg / L, ammonium nitrate (NH4NO3) 1650mg / L, potassium dihydrogen phosphate (KH2PO4) 170mg / L, magnesium sulfate (MgSO4 7H2O) 370mg / L, calcium chloride (CaCl2 2H2O) 330mg / L L;

[0069] The components of trace elements and their corresponding concentrations are as follows: potassium iodide (KI) 0.83mg / L, boric acid (H3BO3) 6.2mg / L, manganese sulfate (MnSO4 4H2O) 22.3mg / L, zinc sulfate (ZnSO4 7H2O) 8.6mg / L, sodium molybdate (Na2MoO4 2H2O) 0.25mg / L, copper sulfate (CuSO4 5H2O) 0.025mg / L, cobalt chloride (CoCl2 6H2O) 0.025mg / L;

[0070] The composition of the iron salt and its corresponding concentration are as follows: disodium ethylenediaminetetraacetic acid (Na2·EDTA) 37.3mg / L, ferrous sulfate (FeSO4.7H2O) 27.8mg / L.

[0071] The components of organic components and their corresponding concentr...

Embodiment 3

[0086] Basic medium configuration:

[0087] Among them, the components of constant elements and their corresponding use concentrations are as follows:

[0088]Potassium nitrate (KNO3) 1500mg / L, ammonium nitrate (NH4NO3) 1200mg / L, potassium dihydrogen phosphate (KH2PO4) 130mg / L, magnesium sulfate (MgSO4 7H2O) 300mg / L, calcium chloride (CaCl2 2H2O) 240mg / L L;

[0089] The components of trace elements and their corresponding concentrations are as follows: potassium iodide (KI) 0.83mg / L, boric acid (H3BO3) 6.2mg / L, manganese sulfate (MnSO4 4H2O) 22.3mg / L, zinc sulfate (ZnSO4 7H2O) 8.6mg / L, sodium molybdate (Na2MoO4 2H2O) 0.25mg / L, copper sulfate (CuSO4 5H2O) 0.025mg / L, cobalt chloride (CoCl2 6H2O) 0.025mg / L;

[0090] The composition of the iron salt and its corresponding concentration are as follows: disodium ethylenediaminetetraacetic acid (Na2·EDTA) 37.3mg / L, ferrous sulfate (FeSO4.7H2O) 27.8mg / L.

[0091] The components of organic components and their corresponding concentra...

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PUM

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Abstract

The invention discloses a carya illinoensis tissue culture propagating method, which is characterized in that stems sections with buds of good young seedlings bred from carya illinoensis seeds are used as explants, the explants are inoculated into a glass culture bottle containing starting culture media after being sterilized, an ordinary fluorescent lamp is used as a light source, the light irradiation is 14hours every day, the temperature is 25 to 28 DEG C, the humidity is 50 to 65 percent, the culture lasts 25 days, test tube bud seedlings are grown through differentiation, then, the test tube bud seedlings are cut and are transferred into a glass culture bottle containing multiplication culture media for carrying out multiplication culture, the multiplication factor is 3.25 to 3.98, the multiplied stem sections are inoculated into a glass culture bottle containing seedling nourishing culture media for carrying out seedling nourishing culture, base part tissues and partial blades are removed after 25 days, 3 to 4 blades are left and are inoculated into a glass culture bottle containing rooting treatment generated root raw base culture media, the rooting culture is carried out for 8 to 15 days , the rooting rate is 90 percent to 95 percent, the plants are transplanted into a substrate with the volume ratio of peat to yellow soil being 1:1 after being cleaned when the root length is about 3cm, and the transplanting survival rate is 80 to 85 percent.

Description

technical field [0001] The invention relates to a method for plant tissue culture and propagation, in particular to a method for tissue culture and propagation of pecans with thin shells. Background technique [0002] Thin-shell hickory [Carya illinoinensis (Wangehn.) K. Koch] is native to eastern North America. It is a deciduous tree of the Juglandaceae Hickory family. It has a relatively wide range of adaptability to soil pH. Alluvial plains or valleys grow rapidly and grow well. It was introduced into my country at the beginning of the 20th century, and it has a history of 100 years. The range of introduction is wide, ranging from Beijing in the north, Hainan Island in the south, Fujian in the east, and Chengdu in the west; the pecans introduced to Jiangsu Province in the early years are growing well and have entered a stable production period . Thin-shell hickory tree is tall and majestic, with beautiful tree posture, and it is an excellent tree species for garden beaut...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 董筱昀蒋泽平黄利斌窦全琴张敏蒋春
Owner JIANGSU ACAD OF FORESTRY
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