The invention discloses a micropterus salmoides fry micro-particle feed and a preparation method thereof. The feed comprises the following raw material components in 1000kg: 290kg-370kg of euphausia superba powder, 130kg-170kg of egg white powder, 110kg-130kg of whitefish powder, 20kg-40kg of tenebrio molitor powder, 140kg of alpha-starch, 100kg of soybean protein concentrate, 50kg of vital gluten, 30kg of fish oil, 20kg of phospholipid oil, 15kg of calcium dihydrogen phosphate, 5.0kg of fish microelements, 2.5kg of choline, 2.0kg of glycine betaine, 1.2kg of bentonite, 1.0kg of fish multi-vitamin, 1.0kg of a mildew preventive, 1.0kg of vitamin C, 0.5kg of carnitine, 0.5kg of an antioxidant and 0.3kg of inositol. The feed has the advantages of low cost, stable yield and quality, and high survival rate of fries and low fry malformation rate, and can completely replace natural biological bait.