The invention discloses a
Pseudomonas fluorescens Y13, and a preparation method and applications thereof. The preparation method comprises the following steps: 1, weighing a soil sample, putting in disinfected normal
saline, oscillating on a constant temperature shaking table, and allowing an obtained solution to stand; 2, taking an obtained supernatant to a Mengjinna organophosphorus
liquid medium; 3, culturing on the constant temperature shaking table, inoculating to a fresh organophosphorus
liquid medium, and continuously culturing; 4,
coating an organophosphorus
solid flat with different
dilution ratios of a
dilution, inverting in a constant temperature culture box, and culturing; and 5, purifying single colonies obtained on the flat, and preserving a finally obtained
pure culture on an LB inclined plane. An above obtained strain is identified as
Pseudomonas fluorescens Y13, is preserved and has a preservation number of CCTCC NO:M2012121. The
Pseudomonas fluorescens Y13 has the advantages of easy preparation obtaining, low cost, increase of the content of soluble
phosphorus in soil, substantial promotion of the
phosphorus element absorption and utilization of plants, high yield of crops, reduction of the planting cost and the agricultural investment, and alleviation of the environmental
pollution caused by the excess application of an agricultural
phosphorus fertilizer.