Pseudomonas fluorescens Y13, and preparation method and applications thereof
A Pseudomonas fluorescens, deposit number technology, applied in the direction of biochemical equipment and methods, applications, microbial-based methods, etc., can solve the problems of inflow of clean water and surface water, water pollution, etc., to reduce planting costs and agriculture Input, easy access, and the effect of mitigating environmental pollution
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Embodiment 1
[0046] Embodiment 1: a kind of preparation method of Pseudomonas fluorescens Y13 and the detection of phosphate-dissolving ability
[0047] 1. Strain isolation:
[0048] A. Weigh 5g of soil sample into 90mL of sterile saline, shake at 180r / min on a constant temperature shaker at 28°C for 30min, and let stand for 1min.
[0049] B, get supernatant 5mL in Montina organophosphate liquid culture medium (glucose 10.00g, (NH 4 ) 2 SO 4 0.50g, MgSO 4 ·7H 2 O 0.30g, NaCl 0.30g, KCl 0.30g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, CaCO 3 5.00g, lecithin 2.00g, distilled water 1000ML, PH7.0-7.2).
[0050] C. Culture on a constant temperature shaker at 28°C at 180r / min for 3 days.
[0051] D. Inoculate into fresh organophosphorus liquid medium at a ratio of 1:100, continue to cultivate for 3 days, take 1 mL of bacterial liquid for 10-fold serial dilution.
[0052] E. Dilute 100 μL of different dilutions (10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 ) were coated on organic phos...
Embodiment 2
[0068] A kind of application of Pseudomonas fluorescens Y13 in dissolving insoluble soil phosphorus, increasing available phosphorus content, improving plant phosphorus nutrition, its application process is:
[0069] 1. Preparation of microbial inoculum:
[0070] 1) Inoculate the Y13 strain stored on the LB slant to a fresh LB slant and activate overnight at 28°C;
[0071] 2) Inoculate into LB liquid medium, culture overnight at 28°C and 180r / min.
[0072] 3) Then inoculate it into a fresh medium at a ratio of 1:100, and continue to cultivate until the cell concentration reaches 10 7 —10 8 up to cfu / mL.
[0073] 4) Take 100g of peat into the Erlenmeyer flask, sterilize at 120°C for 30min at high temperature.
[0074] 5) Add 10mL of bacterial solution to 100g of sterilized peat, and mix evenly. The water content of the fungicide reaches the point where it will form a ball when you hold it by hand, and it will disperse when you touch it.
[0075] 6) Place the mixed bacteri...
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