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High-cadmium-adsorption filamentous fungus Paecilomyces lilacinus XLA, and preparation method and application thereof

A technology of Paecilomyces lilacinus and Paecilomyces lilacinus, which is applied to the preparation of the filamentous fungus Paecilomyces lilacinus XLA, and the field of the filamentous fungus Paecilomyces lilacinus XLA with high adsorption of cadmium, which can solve secondary pollution And deal with the problems of high cost and cumbersome technology, and achieve the effect of good adsorbent regeneration ability, simple treatment process and low cost

Inactive Publication Date: 2013-12-18
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The treatment methods for soil and water polluted by heavy metals include chemical methods, ion exchange methods, adsorption methods, electrolysis methods, chemical redox methods, electrochemical treatment methods, reverse osmosis methods, and membrane separation methods. Limiting factors such as secondary pollution and relatively high treatment costs cannot be put into practical production applications (Kratochvil D, Volesky B, 1998)

Method used

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  • High-cadmium-adsorption filamentous fungus Paecilomyces lilacinus XLA, and preparation method and application thereof
  • High-cadmium-adsorption filamentous fungus Paecilomyces lilacinus XLA, and preparation method and application thereof
  • High-cadmium-adsorption filamentous fungus Paecilomyces lilacinus XLA, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Embodiment 1: A preparation method of the filamentous fungus Paecilomyces lilacinus XLA, the steps of which are: separation, purification and re-screening.

[0046] 1) Isolation of cadmium-resistant strain XLA

[0047] A. Weigh 10g of the potted soil sample used for heavy metal restoration with a heavy metal cadmium content of 50mg / Kg, put it in 90ml of sterile water with 5 0.5mm glass beads, shake it at 200r / min on a constant temperature shaker at 28°C for 30min, statically After standing for 1 min, the supernatant was taken for 10-fold serial dilution;

[0048] B. Spread 100 μL of dilutions of different dilutions on a Martin plate containing 60 mM cadmium, rest for a while, and place it upside down in a constant temperature incubator at 28°C for 5 days;

[0049] C, the microbes grown on the plate are streaked, separated and purified, and then re-applied to the above-mentioned cadmium-containing Martin plate for cultivation;

[0050] D. After 10 times of separation a...

Embodiment 2

[0058] The application of a filamentous fungus Paecilomyces lilacinus XLA in the treatment of heavy metal cadmium pollution is as follows:

[0059] 1) Tolerance detection of filamentous fungus XLA to various heavy metals

[0060] Liquid culture conditions: before inoculation, add a certain amount of filter-sterilized heavy metal mother liquor to the required concentration in the mineral salt (MM) liquid medium, then inoculate the spore suspension of Paecilomyces lilacinus XLA according to 1% inoculum, cultivate for 7 days, and observe its growth status.

[0061] Solid culture conditions: Inoculate spores into mineral salt (MM) liquid medium and culture on a shaking table until mycelial balls just form. The mineral salt (MM) solid plate containing a certain concentration of heavy metals was divided into three equal areas, and mycelium balls with the same diameter were inoculated in each area by spot method, and cultured at 28°C for 7 days. The diameter of mycelial balls was r...

Embodiment 3

[0077] Example 3: The application of a filamentous fungus Paecilomyces lilacinus XLA in the treatment of electroplating wastewater containing heavy metals, its application process:

[0078] Collect live XLA bacteria and prepare dead bacteria as described in Example 2, both of which are used to remove cadmium ions in electroplating wastewater.

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Abstract

The invention relates to a high-cadmium-adsorption filamentous fungus Paecilomyces lilacinus XLA, and a preparation method and an application thereof. The preparation method comprises the following steps: 1, weighing a pot experiment soil sample used for heavy metal restoration and having a content of heavy metal cadmium of 50mg / Kg, adding to sterile water with glass beads, oscillating on a constant temperature shaking table, allowing an obtained solution to stand, taking an obtained supernatant, and carrying out serial dilution; 2, coating a Martin flat containing 60mM of cadmium with different dilution rates of dilutions, allowing the obtained flat to stand for a moment, inverting on a constant temperature culturing box, and culturing; 3, carrying out streaking purification of single colonies growing on the flat, coating the Martin flat with the obtained single colonies, and culturing; 4, carrying out separating screening to obtain the Paecilomyces lilacinus XLA stably resisting cadmium, and preserving on a PDA inclined plane for later use. The Paecilomyces lilacinus XLA is preserved and has a preservation number of CCTCC NO:M2012135. Viable and non-viable organism of the XLA can efficiently adsorb and remove the heavy metal cadmium in heavy metal sewage, so the XLA has the advantages of low cost and simple technology, and has an efficient restoration potential of heavy metal polluted soil and water.

Description

technical field [0001] The invention belongs to the field of water body environment treatment. More specifically, it relates to a filamentous fungus Paecilomyces lilacinus XLA with high adsorption of cadmium, and also relates to a preparation method of the filamentous fungus Paecilomyces lilacinus XLA with high adsorption of cadmium, and also relates to a silky fungus with high adsorption of cadmium. The use of the fungus Paecilomyces lilacinus XLA. Background technique [0002] Since the 20th century, the development of industrialization has greatly promoted the progress of human society, but it has also brought serious environmental pollution to water and soil, especially heavy metal pollution, which in turn affects human health (Hsu et al., 2006 ; Beyer, 1985), a series of heavy metal poisoning and excessive heavy metal events, such as Japan's "Itai disease" caused by cadmium pollution, Minamata disease caused by mercury pollution, etc. (Luigi C., et al, 2006; Ma Xiaofan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B01J20/28C02F1/28C02F1/62B09C1/10C12R1/79
Inventor 陈雯莉黄巧云夏璐朱伟徐兴健张哲轶
Owner HUAZHONG AGRI UNIV
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