The invention provides a traditional Chinese medicine composition for preventing and treating hyperuricemia, a medicine and a preparation method and application thereof, and relates to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition for preventing and treating hyperuricemia mainly comprises lily, cordyceps militaris, spina date seeds, endothelium corneum gigeriae galli, coix seeds, rhizoma imperatae, rhizoma phragmitis and pericarpium citri reticulatae. Through combination of the traditional Chinese medicines having effects of dredging collaterals and removing pyralysis, removing dampness and food retention, treating stranguria and removing stone, reinforcing kidney and tonifying spleen, and reducing fever and causing diuresis, the traditional Chinese medicine composition has effects of protecting, nourishing and dredging kidney, can significantly improve the immunity, can promote blood circulation, can reduce blood lipid, can invigorate the circulation of blood, can accelerate purine decomposition and discharge from the human urine, can effectively reduce blood uric acid content and has no toxic or side effect.