The invention provides a method of manufacturing portunid crab bait. The portunid crab bait is composed of the following raw materials, by weight: 3 to 10% of squid paste, 3 to 10% of seaweed powder, , 1 to 5% of soy lecithin, 1 to 5% of fish oil , 3 to 10% of fish meal, 10 ~ 15% of wheat middling, 30 ~ 50% of soybean meal, 10 ~ 15% of high gluten flour, 0.05 to 0.15% of sodium alginate, 0.005 to 0.015% of amino acids, 0.005-0.015% of attractant small peptides, 1 ~ 5%of monocalcium phosphate, 0.005 to 0.015% of dextran, 0.005 to 0.015% of trimethylamine oxide, 0.005 to 0.015% of sulfobetaine, and 0.5 to 1% of potassium sorbate. According to the invention, raw materials such as the squid paste are employed for preparing compound bait for portunid crabs which are small in yield in Zhoushan but have a high commercial value; and the bait is easy to use and has natural attractant effect, ensuring adequate and stable bait supply for crab catching industry.