The invention relates to a novel cultivation method for butterfly orchid tissue culture seedlings, and belongs to the technical field of flower culture, particularly orchid cultivation. The novel cultivation method comprises the following steps: (1), preparing a cultivation medium: mixing coconut fiber and volcanic rock after being processed to prepare a cultivation medium, or mixing the coconut fiber and float grass imported from Chile, after being processed, to prepare the cultivation medium, or mixing the volcanic rock and the float grass imported from Chile, after being processed, to prepare the cultivation medium, or mixing the coconut fiber, the volcanic rock and the float grass imported from Chile, after being processed, to prepared the cultivation medium, or preparing the coconut fiber after being processed into the cultivation medium; (2), getting a culture flask for loading butterfly orchid tissue culture seedlings, taking the butterfly orchid tissue culture seedlings out of the culture flask, cutting off dead roots and yellow leaves, washing root systems to be clean, and obtaining butterfly orchid tissue culture seedlings to be transplanted; the cultivation medium adopted in the invention has the advantages that degradation products are free from contamination, and good water holding, ventilating, moisture-holding, fertilizer retaining functions are achieved, and the requirements of the growth of the butterfly orchid are met; moreover, the denudation of microbes can be resisted, the probability of rottenness is low, and the cost is low.