The invention discloses an aquifoliaceae cuttage seedling method, which belongs to the field of plant propagation. The insect killing and the sterilization are carried out on nursery soil before the cuttage, then, organic fertilizers are applied, in addition, a layer of yellow soil is spread on the upmost position, and a sunshade net is installed; 5-6-year strong growing aquifoliaceae mother trees are adopted in April to May, branches being 7 to 9cm are adopted as stalk branches, inclined shearing and direct cuttage are adopted, and films are mulched after the sufficient watering; and the moisture preservation, the ventilation and the water and fertilizer management are enhanced in summer, the mulched films are gradually opened in autumn, the films are mulched in winter, in addition, straws are covered for realizing the anti-freezing effect, the mulched films are gradually opened in spring, the water and fertilizer management is enhanced, and the transplanting and fixed planting capability can be realized in spring of the next year. The aquifoliaceae cuttage seedling method has the advantages that the pest disease prevention and the growth reinforcement are emphasized in the early stage, the safe living through the summer and the winter of cuttage seedlings is realized, meanwhile, the water and fertilizer management is enhanced, and the transplanting and fixed planting capability can be realized in spring of the next year.