The invention discloses a method for detecting systemic
pesticide dimethoate residue. The method comprises the following steps: step a, crushing a sample to be detected to obtain a first sample; step b, adding a
sodium salt solution and
chloroform into the first sample, performing centrifugal treatment until the solution is obviously layered, and taking a
chloroform phase solution to obtain a first sample solution; step c, blow-
drying the first sample liquid to obtain a first substance to be detected; step d, adding
diethylamine into the first substance to be detected, redissolving, adding an
oxidizing agent, adjusting the temperature to 80 DEG C, standing to obtain a second sample solution, and blow-
drying to obtain a second substance to be detected; step e, adding an
ether organic solvent into the second substance to be detected, redissolving, slowly adding a
buffer solution, adding an acetylcholin
esterase reagent, and standing; step f, adding a substrate and a color developing agent, standing for 3 minutes, observing the color change of a lower layer after the solution is layered, shaking, standing, and observing the color change of the lower layer after the solution is layered; and step g, judging a result.