The invention provides a traditional Chinese medicinal preparation for treating tenovaginitis of flexor digitorum, as well as a preparation and application thereof. The traditional Chinese medicinal preparation comprises the following raw materials: cactus, cassia twig, mulberry twig, perilla leaf, curcuma zedoary, corydalis tuber, pseudo-ginseng, cortex lycii radicis, radix clematidis, garlic, mirabilite, myrrh, frankincense, pawpaw, safflower, rheum officinale, eucommia bark, dipsacus asperoids, notopterygium root, asarum, red peony root, lycopodium clavatum, garden balsam stem and rhizoma drynariae. The traditional Chinese medicinal preparation can be used for tenosynovitis syndromes caused by tendon and muscle injury due to overuse and meridian invasion due to cold and damp, meridian blockage, unsmooth circulation of vital energy and blood, and qi-stagnation and blood stasis, has the medical effects of activating blood circulation to remove stasis, diminishing swelling and relieving pain, dredging channels, regulating qi and blood, smoothing circulation of qi and blood, improving local circulation, repairing injured tissues and the like, and can effectively prevent relapse of the disease.