The invention relates to a preparation method of a multilayer metal grating. The preparation method comprises the following steps of: preparing or selecting an imprinting template; spin coating a layer of imprinting glue on an upper surface of a substrate; plating a metal layer on a surface of the imprinting glue; coating a layer of protection film on an upper surface of a metal layer; placing the imprinting template on an upper surface of the protection film, and carrying out nano-imprinting; and after curing the imprinting glue, removing the imprinting template to obtain a double-layer metal grating or the multilayer metal grating. According to the preparation method of the multilayer metal grating, a step of pattern transfer is omitted, the process is simpler, the cost is lower, a metal film obtained through the method cannot be coated on a side wall of a pattern to affect the transmission efficiency, the shape of a cross section of the metal film is strictly matched with the shape of a cross section of the imprinting template, and compared with adoption of a traditional method, the fidelity of the pattern and the TM transmission rate and the polarization rejection ratio of the grating are higher.